Screening of mungbean (<i>Vigna radiata</i>) germplasm for resistance to<i>Mungbean yellow mosaic virus</i>using agroinoculation

Manickam, S.; Karthikeyan, Adhimoolam; Nagarajan, P.; Raveendran, M.; Senthil, N.; Pandiyan, M.; Angappan, K.; Ramalingam, J R; Bharathi, M.; Rabindran, R.; Veluthambi, K.; Balasubramanian, P.

doi:10.1080/07060661.2013.827134

Cited by 31 publications

(26 citation statements)

References 9 publications

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“…The low level of disease exhibited by A-555 at field level may be due to the inability of whitefly to introduce a sufficient quantity of virus particles into the plants to cause a severe disease response as reported earlier in case of tomato against tomato leaf curl geminivirus and in case of non-Bt Gossypium specie against CLCuBuV (Akhtar et al, 2010; Tripathi and Varma 2002). Under field conditions, natural infection may not produce accurate results even if the studies are conducted in field with high vector populations (Sudha et al, 2013). An effective screening method is a critical component of an accurate disease assay for quantifying the levels of disease resistance among crop cultivars (Kim et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Akhtar

Hussain

Mahmood-ul-Hassan

et al. 2015

The Plant Pathology Journal

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Bt-cotton germplasm, consisting of 75 genotypes was evaluated against cotton leaf curl disease (CLCuD) under high inoculum pressure in the field and using graft inoculation in glasshouse by visual symptom scoring assessments. None of the tested genotype was found disease free under both evaluation tests. Under field conditions in 2011, 3 genotypes were found resistant, 4 moderately resistant, 3 tolerant, 2 moderately susceptible and one susceptible; in 2012, 3 genotypes were tolerant, 7 moderately susceptible, 5 susceptible and 38 highly susceptible; in 2013, one was moderately susceptible and 51 were highly susceptible with varying degree of percent disease index (PDI) and severity index (SI). However, through graft evaluation in glasshouse, none of the graft inoculated plant was symptomless. All tested genotypes showed disease symptoms with SI values ranging between 5.0 and 6.0, and latent period between 12 and 14 days. Of the 75 genotypes evaluated using graft inoculation, 11 were found susceptible with SI values of 5.0 to 5.4 while remaining 64 were highly susceptible with SI values of 5.5 to 6.0. Inoculated plants of all tested genotypes exhibited severe disease symptoms within 10 days after the appearance of initial symptoms. No reduction in SI value was observed until the end of the experiment i.e., 90 days after grafting. Information generated under the present study clearly demonstrates that no sources of resistance to CLCuD are available among the tested Bt-cotton genotypes. So, a breeding programme is needed to introgress the CLCuD-resistance from other resistant sources to agronomically suitable Bt-cotton genotypes.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Akhtar

Hussain

Mahmood-ul-Hassan

et al. 2015

The Plant Pathology Journal

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“…Because of constraints in natural screening and the chances of mixed infection, implementing an artificial screening technique is strongly needed to mitigate this issue. Several groups, including Jacob et al [147], Gupta et al [118], Sudha et al [15], and Sai et al [51], have used artificial screening techniques combined with agro-inoculation for the strict screening of breeding material against the specific YMD-causing viruses. In our opinion, agro-inoculation coupled with the spreader row and force-feeding techniques will give more precise information about the true resistant genotypes by confirming all the involved parameters, like the susceptible host, natural environment, and the presence of virus and vector.…”

Section: Durable Sources Of Lymv Resistancementioning

confidence: 99%

“…YMD was first reported in lima bean (Phaseolus lunatus) in 1940 [10] and was subsequently discovered in other plants, such as Dolichos bean (Dolichos lablab) [11,12] and mungbean [13]. It creates malformations in mungbean plants, which in turn inhibit photosynthetic efficiency and plant growth, ultimately leading to significant yield losses [7,14,15]. Khattak et al [16] reported that YMD might lead to up to 32%-78% reduction in grain yield of mungbean.…”

Section: Introductionmentioning

confidence: 99%

Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

Singh¹,

Singh

Pratap

et al. 2019

Agronomy

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Yellow mosaic disease (YMD) affects several types of leguminous crops, including the Vigna species, which comprises a number of commercially important pulse crops. YMD is characterized by the formation of a bright yellow mosaic pattern on the leaves; in severe forms, this pattern can also be seen on stems and pods. This disease leads to tremendous yield losses, even up to 100%, in addition to deterioration in seed quality. Symptoms of this disease are similar among affected plants; YMD is not limited to mungbean (Vigna radiata L. Wilczek) and also affects other collateral and alternate hosts. In the last decade, rapid advancements in molecular detection techniques have been made, leading to an improved understanding of YMD-causing viruses. Three distinct bipartite begomoviruses, namely, Mungbean Yellow Mosaic India Virus (MYMIV), Mungbean Yellow Mosaic Virus (MYMV), and Horsegram Yellow Mosaic Virus (HgYMV), are known to cause YMD in Vigna spp. Vigna crops serve as an excellent protein source for vegetarians worldwide; moreover, they aid in improving soil health by fixing atmospheric nitrogen through a symbiotic association with Rhizobium bacteria. The loss in the yield of these short-duration crops due to YMD, thus, needs to be checked. This review highlights the discoveries that have been made regarding various aspects of YMD affecting mungbean, including the determination of YMD-causing viruses and strategies used to develop high-yielding YMD-resistant mungbean varieties that harness the potential of related Vigna species through the use of different omics approaches.

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“…Screening the germplasms using different MYMV isolates through agroinoculation technique is the effective method for assessing the genetic diversity for MYMV resistance in mungbean (Karthikeyan et al 2011). Recently, Sudha et al (2013b) screened the mungbean germplasms using two different MYMV isolates. The results show that among the seventy eight mungbean genotypes screened, four genotypes exhibited resistance to the isolates of MYMV.…”

Section: Pathogen-derived Resistancementioning

confidence: 99%

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

Karthikeyan

Shobhana

Manickam

et al. 2014

International Journal of Pest Management

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Mungbean yellow mosaic virus (MYMV) disease is one of the most vicious diseases of green gram and has been renowned in India for more than five decades. It is caused by a group of geminiviruses belonging to the genus, begomovirus of the family, Geminiviridae. They are transmitted through whitefly in a persistent manner. The economic losses due to this virus account up to 85% in green gram which is spreading faster towards newer areas. The escalating economic importance of MYMV has resulted in the call for accurate detection and identification procedures that inspire rigorous research efforts focussing on the biology, diversity and epidemiology of the virus, so that viable management strategies could be designed. Breeding for resistance or tolerance appears to be the best approach to control this disease. However, the commercially offered genotypes are only partially resistant. Therefore, the hunt for newer sources of disease resistance needs to be intensified. This review updates all the accessible information on MYMV and outlines the areas in which advance research is indispensable.

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Screening of mungbean (Vigna radiata) germplasm for resistance toMungbean yellow mosaic virususing agroinoculation

Cited by 31 publications

References 9 publications

Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

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