Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Akhtar, Khalid Pervaiz; Hussain, Muhammad Arshad; Mahmood-ul-Hassan, .; Sarwar, Muhammad; Sarwar, Nighat

doi:10.5423/ppj.oa.01.2015.0014

Cited by 9 publications

(9 citation statements)

References 21 publications

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“…Newly growing cotton plants were inoculated with the virus strain (CLCuV). Data were recorded on the ratio of effective grafts, rate of illness transmission, inactive sum (aver-age time needed for initial symptom look once grafting), and sort of indications created ninety days post vaccination (Akhtar et al, 2015).…”

Section: Virus Transmission and Identificationmentioning

confidence: 99%

Expression of Oxidative Enzymes in Cotton Plant under Biotic Stress

Tariq¹,

Rizwan²,

Rafique³

et al. 2020

IJSRP

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Cotton leaf curl disease caused by the Cotton Leaf Curl Gemini Virus (CLCuV) is limited to Asia and Africa. This study was conducted in green house to check the role of antioxidant enzymes under diseased conditions. Four cotton cultivars (Gossypium hirsutum) were planted with two replications to check the expression in healthy and disease infected plants. The estimations of total soluble protein contents and the activities of peroxidases (POX), catalases (CAT), proteases and superoxide dismutase (SOD) were studied in leaves with 8 genotypes of cotton, four susceptible and four are disease infected. Disease symptoms were shown less intense in disease infected genotypes but severe in highly susceptible genotypes. The results showed that protein, POX, CAT, proteases and SOD played major role in disease resistance against CLCuV. The SOD, protein contents and CAT activities showed lower values in disease infected genotypes while they decreased significantly in susceptible genotypes. The CAT was found to be increased in both genotypes with high percentage in infected genotypes as compared to healthy. The results showed that significantly higher concentrations of total phenols and higher activities of protease the SOD, CAT and TFC in disease infected genotypes after infection with CLCuV. Statistically the ANOVA was performed to check the levels of above mentioned enzymes against plant resistance. These findings could be considered as biochemical markers for studying plant-virus compatible and incompatible interactions.

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Section: Virus Transmission and Identificationmentioning

confidence: 99%

Expression of Oxidative Enzymes in Cotton Plant under Biotic Stress

Tariq¹,

Rizwan²,

Rafique³

et al. 2020

IJSRP

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“…The Bt-cotton hybrids were introduced in NW India during the year of 2005 and after that a large number of Btcotton hybrids are introduced for cultivation in this region. Mostly all of the Bt-cotton hybrids are recently observed to be highly susceptible to CLCuD (Akram et al 2013, Godara et al 2015, Akhtar et al 2015. Akram et al (2013) reported that Bt-cotton hybrids are more susceptible host for whiteflies than non-Bt cottons in Pakistan resulting in higher disease incidence in Bt-hybrid cotton.…”

Section: Disease Incidence and Boll Number Reduction In Clcud Infecte...mentioning

confidence: 99%

Recent status and distribution pattern of cotton leaf curl disease in Northwest India: an alarming situation in future cotton cultivation

et al. 2017

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A survey was made to study cotton leaf curl disease (CLCuD) incidence in cotton growing areas of Haryana, Punjab and Rajasthan in Northwest (NW) India during the years of 2013 and 2014. The present study revealed higher overall CLCuD incidence of 77.5% with higher overall boll number reduction (BNR) of 36.9% in 2013 compared to incidence of 49.6% with 7.6% BNR in 2014 in Haryana. In Rajasthan the disease incidence of 55.9% and 21.6% BNR in 2013 when compared to 10.8% of incidence and 2.9% BNR in 2014 was recorded. The overall CLCuD incidence and BNR in cotton growing areas of Punjab were more or less similar for both the years of 2013 and 2014, where disease incidence of 54.1% with BNR 14.6% in 2013 and disease incidence 57.8% with BNR of 15.9% in 2014 was recorded. All the 11 Bt-cotton hybrids from the farmerâ€™s fields of Sri Ganganagar and Sirsa districts surveyed were highly susceptible to CLCuD in both the years; showing 100% disease incidence with BNR of 32.3-82.3% in 2013 and 49.2-100% with BNR of 8.7-17.4% in 2014. Infectivity study through whitefly (Bemisia tabaci) and polymerase chain reaction (PCR) of ORF V1 (CP gene) determined that CLCuD in NW India is caused by whitefly transmitted CLCuD-begomoviruses. Sequence analysis of CP gene indicated that at least three CLCuD-begomoviruses variants appeared in this cotton growing region. The increased CLCuD incidence with huge yield loss and occurrence of CLCuD-begomovirus variants reported in the present is an alarming situation for the profitable cultivation of cotton in north India.

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“…The sick plot technique was used by planting one row of highly susceptible cultivar S-12 after every three rows of screening germplasm to maximize CLCuD inoculum(Iqbal et al 2014). The disease severity was scored as percent disease severity index (DSI) at 60, 90, and 120 days after sowing (DAS), as previously described(Akhtar et al 2015).CLCuD incidence (%) = (Sum of all disease) / (Total number of plants) × 100/6^* *= Rating of maximum disease severity Genomic DNA extraction and SSR analysis The youngest leaves of the parental population and F2 progeny plants were collected in ziplock plastic bags for total genomic DNA extraction. The standard CTAB DNA extraction protocol was followed (Paterson et al 1993).…”

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confidence: 99%

Mapping of quantitative trait loci (QTLs) controlling cotton leaf curl disease (CLCuD) resistance in Upland cotton

Sattar

Javed

Hussain

et al. 2022

Preprint

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Cotton leaf curl disease (CLCuD), caused by begomoviruses, is a major threat to cotton (Gossypium hirsutum L.) production, especially in Asia and Africa. The use of marker-assisted breeding to utilize host plant resistance can be the best sustainable approach to tackle CLCuD. Because most of the available cotton germplasm is susceptible to CLCuD, so it demands the identification of potentially new quantitative trait loci (QTLs) in the indigenous germplasm. The current study was designed to construct a broader genetic linkage map of bi-parental F2:F3 populations developed from a highly tolerant MNH 886 and a highly susceptible S-12 cotton cultivars. One hundred seven (107) CLCuD-associated simple sequence repeat (SSR) marker alleles were identified as polymorphic and eight new QTLs were found on chromosomes 11, 19 and 21. On homologous chromosomes 11 and 17, the QTLs qCL-11-120d2 and qCL-21-m2 were detected in both F2 and F3 populations. Another QTL on chromosome 11, qCL-11-m3, showed the highest phenotypic variance (PV%) of 16.66% and LOD score > 3. Other QTLs on chromosome 11 were qCL-11-m2, qCL-11-120d3. On chromosome 19, three QTLs (qCL-19-m2, qCL-19-90d2 and qCL-19-120d3) were detected with PV% >8 and LOD score > 3. The CLCuD-associated QTLs identified in this study can contribute to develop CLCuD-resistant cultivars using marker assisted selection and can help to fine-tune the molecular mapping of the QTLs on the cotton genome.

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Evaluation of Bt-cotton Genotypes for Resistance to Cotton Leaf Curl Disease under High Inoculum Pressure in the Field and Using Graft Inoculation in Glasshouse

Cited by 9 publications

References 21 publications

Expression of Oxidative Enzymes in Cotton Plant under Biotic Stress

Expression of Oxidative Enzymes in Cotton Plant under Biotic Stress

Recent status and distribution pattern of cotton leaf curl disease in Northwest India: an alarming situation in future cotton cultivation

Mapping of quantitative trait loci (QTLs) controlling cotton leaf curl disease (CLCuD) resistance in Upland cotton

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