Screening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities

Asnani, Ari; Ryandini, Dini; Suwandri,

doi:10.1088/1757-899x/107/1/012056

Cited by 11 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on phenotypic and genotypic characteristic variations, isolate VMS-A10 was designated as Streptomyces parvulus strain sankarensis-A10 (NCIM-5601, GenBank-KT906299). Similarly, Streptomyces fradiae strain RSU15 has been reported to produce hydrolytic enzymes namely amylase, protease, lipase, and cellulose [31]. A terrestrial actinomycete Streptomyces corchorusii strain UCR3-16 was found to be positive for the production of chitinase, β-1,3-glucanase, β-1,4-glucanase, lipase and protease [32].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

The significance and frequency of marine microorganisms as producers of bioactive metabolites-a natural source of drug discovery had varied significantly during the last decades, making marine ecosystem a huge treasure trove of novel isolates and novel compounds. Among the twelve actinomycetes isolated from marine sediment sample (Lat. 17°41′962″N, Long. 83°19′633″E), amylase, protease, lipase and cellulase activities were exhibited by 8,7,4,3 isolates respectively. Five isolates exhibited l-asparaginase activity, while 5, 6, 2 isolates exhibited antibacterial, antifungal and antimicrobial activities respectively. One isolate VMS-A10 efficiently producing alpha-amylase (25.53 ± 0.50 U/mL), protease (19.26 ± 0.25 U/mL), lipase (36.25 ± 0.10 U/mL), cellulase (14.43 ± 0.513 U/mL), l-asparaginase (0.125 ± 0.004 U/mL), antimicrobial metabolites against B. subtilis (503.33 ± 5.77 U/mL), S. aureus (536.66 ± 5.77 U/mL), E. coli (533.33 ± 5.77 U/mL), P. aeruginosa (500.00 ± 10.0 U/mL), MRSA (538.33 ± 5.77 U/mL), C. albicans (353.33 ± 11.54 U/mL) and A. niger (443.33 ± 15.27 U/mL) was selected, identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rDNA sequence, designated as Streptomyces parvulus strain sankarensis-A10 and sequencing product (1490 bp) was deposited in the GenBank database under accession number KT906299, Culture Deposit No: NCIM-5601. Isolation and characterization of each potential actinobacteria having immense industrial and therapeutic value on an unprecedented scale from marine sediments of Visakhapatnam coast will have a burgeoning effect.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…The filtrate obtained was an extracellular pigment and pellets were extracted using methanol to obtain intracellular pigments. The filtrate was extracted with 70% methanol (1: 1) added and left for 12 hours to obtain pure pigment (Asnani et al 2016). In this study, the extracellular pigment was obtained.…”

Section: Fermentation Mediamentioning

confidence: 99%

The biological prospective of red-pigmented bacteria cultured from contaminated agar media

et al. 2021

View full text Add to dashboard Cite

Abstract. Arifiyanto A, Afriani H, Putri MH, Damayanti B, Riyanto CL. 2021. The biological prospective of red-pigmented bacteria cultured from contaminated agar media. Biodiversitas 22: 1152-1159. Contaminated agar media was often depleted due to the growth of microbes, which is undesirable for culture. However, the contaminating microbes usually have a distinctive morphology. This research aimed to identify the potential of red-pigmented bacteria originated from the contamination of Drosophila melanogaster larvae. Fruit fly larvae that grow on contaminated tryptic soy agar were accompanied by the appearance of the red-pigmented bacteria colony. The bacterial colonies were purified by the re-streaking method on tryptic soybean agar. This strain was characterized morphologically, biochemically, and molecularly. Results showed that the MBC1 strain was identified as Serratia marcescens. The various metal susceptibility tests at 25 ppm did not affect the growth of the MBC1 strain. Meanwhile, it was also able to inhibit the growth of certain pathogens such as Aspergillus niger, Candida sp., Fusarium sp., and Rigidoporous sp. Strain MBC1 was able to produce antioxidant compounds. The lipolytic and amylolytic activity can be developed for bioremediate agriculture waste and biosurfactant production.

show abstract

“…The secondary metabolites produced by actinomycetes include a wide range, i.e. polyene (Ambavane et al 2014;Vartak et al 2014), terpenoids (El-Sayed and Awad, 2013), phenolic (Belghit et al 2016), polyketide (Oja et al 2015;Asnani et al 2016;Abdelmohsen et al 2017), fenazin (Abdelmohsen et al 2014), piperazin (Abdelmohsen et al 2017), and nonpolyene (Augustine et al 2005). Many actinomycetes showing antimicrobial activities were found in marine habitats (Fenical and Jensen, 2006).…”

Section: Introductionmentioning

confidence: 99%

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

et al. 2021

View full text Add to dashboard Cite

Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of the Gram-positive bacteria which are widely distributed and produce many secondary metabolites including those known as antibiotics, antifungals, anticancer, and antimalarials agents. The secondary metabolites of actinomycetes are abundant, which include many active compounds that have been identified because of the large diversity in the actinomycetes phylum. This study aimed to identify and screen collected by Indonesian Culture Collection (InaCC) from Bojong Gede and Pramuka Island, Jakarta, Indonesia as antibacterial and antifungal agents. Primary screening was done on 16 actinomycetes isolates by well-agar diffusion method. Antimicrobial activity was tested by using micro broth dilution methods to determine minimum inhibitory concentration (MIC). Molecular identification into level genera and species was determined by 16S rRNA gene sequencing. Out of 16 actinomycetes isolates used, 4 isolates have activity against Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538, Bacillus subtilis BTCC B-612, and Escherichia coli BTCC B-614, specifically InaCC A758, InaCC A759, InaCC A760 and InaCC A765 isolates. InaCC A758 have highest antimicrobial activity against mentioned microbial with MIC value at 50 μg/mL, 6.25 μg/mL, 31.25 μg/mL and 3.125 μg/mL, respectively. Three genera were found from the samples: i.e. Streptomyces (80%), Microbispora (13%) and Nocardia (6%). Based on 16S rRNA gene identification, the active isolates of actinomycetes InaCC A758, InaCC A759, InaCC A760 and InaCC A765 were similar to Streptomyces badius, Streptomyces olivaceus, Streptomyces sanyensis, and Nocardia otitidiscaviarum, respectively. The secondary metabolites of actinomycetes extracts from Pramuka Island can be potentially developed as antifungal and antibacterial agents.

show abstract

Screening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities

Cited by 11 publications

References 4 publications

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

The biological prospective of red-pigmented bacteria cultured from contaminated agar media

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

Contact Info

Product

Resources

About