The biological prospective of red-pigmented bacteria cultured from contaminated agar media

Abstract

Abstract. Arifiyanto A, Afriani H, Putri MH, Damayanti B, Riyanto CL. 2021. The biological prospective of red-pigmented bacteria cultured from contaminated agar media. Biodiversitas 22: 1152-1159. Contaminated agar media was often depleted due to the growth of microbes, which is undesirable for culture. However, the contaminating microbes usually have a distinctive morphology. This research aimed to identify the potential of red-pigmented bacteria originated from the contamination of Drosophila melanogaster larvae. Fruit fly larvae that grow on contaminated tryptic soy agar were accompanied by the appearance of the red-pigmented bacteria colony. The bacterial colonies were purified by the re-streaking method on tryptic soybean agar. This strain was characterized morphologically, biochemically, and molecularly. Results showed that the MBC1 strain was identified as Serratia marcescens. The various metal susceptibility tests at 25 ppm did not affect the growth of the MBC1 strain. Meanwhile, it was also able to inhibit the growth of certain pathogens such as Aspergillus niger, Candida sp., Fusarium sp., and Rigidoporous sp. Strain MBC1 was able to produce antioxidant compounds. The lipolytic and amylolytic activity can be developed for bioremediate agriculture waste and biosurfactant production.

Antioxidant Activity of Endophytic Bacteria isolated from (Pyrrosia piloselloides) (L) M.G. Price

“…After filtering using Whatman paper number 1, extracts are obtained using a rotary evaporator. The extracted samples were preserved for further analysis 13,14 .…”

Effect of Indole Acetic Acid (IAA) by Serratia marcescens strain MBC1 on Soybean (Glycine max L.) Germination

“…Bakteri Serratia marcescens strain MBC1 berasal dari cemaran larva Drosophila melanogaster yang tumbuh pada media TSA (Tryptic Soy Agar). Berdasarkan identifikasi Serratia marcescens strain MBC1 menunjukkan uji oksidase negatif dan uji katalase positif [3]. Strain MBC1 memiliki metabolit sekunder alkaloid dan saponin [4].…”

Analisis Senyawa Bioaktif Ekstrak Metabolit Sekunder Serratia marcescens strain MBC1

“…S. marcescens strain MBC1 mengandung pigmen berwarna merah yang disebabkan oleh aktivitas amilolitik dan lipolitik yang dapat dimanfaatkan dalam bidang pertanian maupun sebagai biosurfaktan. Selain itu, bakteri ini juga memiliki kemampuan sebagai antioksidan [4]. Hasil penelitian lain melaporkan bahwa bakteri ini memiliki kemampuan dalam mendegradasi enzim lipase, mannanase, selulase, amilase, dan protease [5].…”

“…Isolat S. marcescens strain MBC1 di sub-kultur pada media TSA (Tryptic Soy Agar) kemudian diinkubasi di inkubator pada suhu 37 o C selama 7 hari [4].…”