The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

Abstract

Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of the Gram-positive bacteria which are widely distributed and produce many secondary metabolites including those known as antibiotics, antifungals, anticancer, and antimalarials agents. The secondary metabolites of actinomycetes are abundant, which include many active compounds that have been identified because of the large diversity in the actinomycetes phylum. This study aimed to identify and screen collected by Indonesian Culture Collection (InaCC) from Bojong Gede and Pramuka Island, Jakarta, Indonesia as antibacterial and antifungal agents. Primary screening was done on 16 actinomycetes isolates by well-agar diffusion method. Antimicrobial activity was tested by using micro broth dilution methods to determine minimum inhibitory concentration (MIC). Molecular identification into level genera and species was determined by 16S rRNA gene sequencing. Out of 16 actinomycetes isolates used, 4 isolates have activity against Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538, Bacillus subtilis BTCC B-612, and Escherichia coli BTCC B-614, specifically InaCC A758, InaCC A759, InaCC A760 and InaCC A765 isolates. InaCC A758 have highest antimicrobial activity against mentioned microbial with MIC value at 50 μg/mL, 6.25 μg/mL, 31.25 μg/mL and 3.125 μg/mL, respectively. Three genera were found from the samples: i.e. Streptomyces (80%), Microbispora (13%) and Nocardia (6%). Based on 16S rRNA gene identification, the active isolates of actinomycetes InaCC A758, InaCC A759, InaCC A760 and InaCC A765 were similar to Streptomyces badius, Streptomyces olivaceus, Streptomyces sanyensis, and Nocardia otitidiscaviarum, respectively. The secondary metabolites of actinomycetes extracts from Pramuka Island can be potentially developed as antifungal and antibacterial agents.

Phylogenetic analysis of Streptomyces producing antimicrobial agent isolated from Kukup Beach Sand, Yogyakarta, Indonesia

“…Another study found one isolate (InaCC A765) of three Actinomycetes isolates from a sand beach in Pramuka Island, Kepulauan Seribu, Jakarta, Indonesia, had antimicrobial activity against S. aureus ATCC 6538, B. subtilis BTCC B-612, and E. coli BTCC B-614, with zones of inhibition measuring 20, 18 and 20 mm, respectively. InaCC A765 is closely related to Nocardia otitidiscaviarum Snijders 1924 strain NBRC 14405 (MN826189); it has a similarity of 96.06% (Setiawati et al 2021).…”

Exploring the potency of Streptomyces koyangensis strain SHP 9-3 isolated from the soil of Enggano Island (Indonesia) as an antibacterial source

“…Streptomyces koyangensis SHP 9-3 from the solid medium was inoculated into 100 mL of ISP-2 and incubated for 72 hours at 30°C and 130 rpm in a shaker incubator (Setiawati et al 2021). Afterward, 90 mL of production medium and 10 mL of pre-culture stock were added to a 500 mL baffled flask (Susanti et al 2019).…”

“…Antibiotic resistance is still a global concern in the health sector (Jakubiec-Krzesniak et al 2018;Atikana et al 2021;Setiawati et al 2021). Approximately 700,000 people die annually from infectious diseases and drug resistance (Ragheb et al 2019).…”