Screening antioxidant capacity of Mexican maize (Zea mays L.) landraces with colored grain using ABTS, DPPH and FRAP methods

Ramírez-García, Orlando; Salinas-Moreno, Y.; Santillán-Fernández, Alberto; Sumaya-Martínez, M. T.

doi:10.1007/s42976-021-00221-6

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…ABTS and DPPH radical scavenging activities of the amino acids were analyzed as previously described [16]. The ABTS radical scavenging activity was expressed as the ascorbic acid equivalent antioxidant capacity (µg AEAC/mg) from a standard curve obtained using ascorbic acid.…”

Section: Analysis Of Radical Scavenging Activity Of Amino Acidsmentioning

confidence: 99%

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice

Kim,

Suh,

Hong

et al. 2023

Antioxidants

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Excessive alcohol consumption increases oxidative stress, leading to alcoholic liver disease. In this study, the protective effects of a mixture of cysteine and glutathione against ethanol-induced hangover and liver damage were evaluated in mice and HepG2 cells. Ethanol (2 mL/kg) was orally administered to the mice 30 min before receiving the test compounds (200 mg/kg), and the behavioral and oxidative stress-related biochemical parameters altered by ethanol were analyzed. Acute ethanol administration increased anxiety behavior and decreased balance coordination in mice (p < 0.001); however, a mixture of cysteine and glutathione (MIX) in a 3:1 ratio improved alcohol-induced behavior more effectively than the individual compounds (p < 0.001). The MIX group showed higher ethanol-metabolizing enzyme activity than the control group (p < 0.001) and significantly suppressed the elevation of serum alcohol (p < 0.01) and acetaldehyde (p < 0.001) levels after 1 h of ethanol administration. In HepG2 cells, 2.5 mM MIX accelerated ethanol metabolism and reduced cytochrome P450 2E1 mRNA expression (p < 0.001). MIX also increased the expression of antioxidant enzymes through the upregulation of nuclear erythroid 2-related factor 2 (Nrf2) signaling and consequently suppressed the overproduction of reactive oxygen species and malondialdehyde (p < 0.001). Collectively, MIX alleviates the hangover symptoms and attenuates the alcohol-induced oxidative stress by regulating the Nrf2 pathway.

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Section: Analysis Of Radical Scavenging Activity Of Amino Acidsmentioning

confidence: 99%

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice

Kim,

Suh,

Hong

et al. 2023

Antioxidants

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“…Este efecto se observó a partir de las concentraciones altas (1.0 y 1.5 %), ya que las tortillas elaboradas con las concentraciones bajas (0.5 y 0.7 %) mostraron igual CA; sin embargo, no existieron diferencias estadísticas (P ≥ 0.05) de la CA de las tortillas entre las diferentes concentraciones de álcali utilizadas (Cuadro 4), atribuido a la liberación de ácidos fenólicos que ocurre con la nixtamalización. Los valores de CA obtenidos con el método ABTS fueron más elevados que los obtenidos con el método FRAP, lo que coincide con lo informado para maíz por otros autores (Ramírez-García et al, 2022). En las tortillas AZM elaboradas con diferentes dosis de álcali, la CA estuvo estrechamente ligada con el CAT de las tortillas.…”

Section: Tipo De Maízunclassified

COLOR Y CARACTERÍSTICAS NUTRACÉUTICAS DE TORTILLAS DE MAÍCES (Zea mays L.) NATIVOS PREPARADAS CON DIFERENTES DOSIS DE ÁLCALI Y REFRIGERADAS

Gutiérrez-Llanos,

Alemán-de la Torre,

Salinas-Moreno

et al. 2023

RevFitotecMex

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La nixtamalización del grano de maíz con antocianinas causa pérdida considerable (50-75 %) de estos compuestos en su proceso de transformación hacia tortillas. Existe poca información del efecto del pH alcalino de la tortilla durante su almacenamiento en refrigeración. El objetivo del presente estudio fue determinar el efecto de diferentes concentraciones de álcali durante la nixtamalización sobre color, variables fenólicas y capacidad antioxidante (CA) de tortillas elaboradas con granos de maíces con antocianinas y almacenadas en refrigeración. Se utilizó una variedad de maíz de grano azul morado (AZM) y otra de grano rojo cereza (RC). Las concentraciones de álcali fueron 0.5, 0.7, 1.0 y 1.5 %, y los tiempos de almacenamiento de las tortillas fueron de 1, 6 y 12 días. Las variables fenólicas incluyeron contenido de antocianinas totales (CAT) y fenoles solubles totales (FST). La capacidad antioxidante (CA) se midió con los métodos ABTS [ácido 2,2′-azinobis (3-etilbenzotiazolina-6-sulfónico)] y FRAP (poder antioxidante reductor férrico). El efecto del álcali sobre el color de la tortilla fue diferente de acuerdo con el color del grano. La tortilla de maíz AZM intensificó su color azul al incrementarse el álcali a 1.0 %; en concentraciones mayores predominó un tono amarillento, mientras en la RC, el color rosado prevaleció hasta la concentración de 0.7 %, mayores concentraciones indujeron tono amarillento. Al final del almacenamiento, la tortilla AZM perdió entre 61 y 76.8 % de su CAT inicial, en la tortilla RC las pérdidas variaron entre 23 y 45.5 %, según la concentración de álcali empleada. La CA de las tortillas se conservó durante el almacenamiento cuando se emplearon concentraciones bajas de álcali. El efecto de la cantidad de álcali durante la nixtamalización sobre el color, CAT, FST y CA difiere según el color del grano, e influye en la estabilidad de las antocianinas durante el almacenamiento de las tortillas.

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“…Hydroxyl radicals are the most active free radicals and can react with most biomolecules including proteins, lipids and nucleic acids [33]. Fe 2+ chelating ability, ABTS radical scavenging activity and DPPH radical scavenging activity were selected to evaluate the antioxidant properties of gel films [34,35]. Analysis of the in vitro antioxidant activity results in Figure 3A revealed that the multi-ionic cross-linked gel films had some scavenging and reducing ability for all three, and the 3%COS+Z+S group had a significant scavenging effect on DPPH radicals, even higher than the positive control Vitamin C, while that of the 3%COS+C+Z+S group was relatively poor.…”

Section: Antioxidant Activity Of Cos/sa/nm 2+ Pdfmentioning

confidence: 99%

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

et al. 2022

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The development of an efficient and convenient material to improve skin tissue regeneration is a major challenge in healthcare. Inspired by the theory of moist wound healing, portable chitooligosaccharide (COS)/sodium alginate (SA) dual-net gel films containing multiple metal ions were prepared by a casting and in -situ spray method, which can be used to significantly promote wound healing without the use of therapeutic drugs. A variety of divalent cations was introduced in this experiment to improve the advantages of each metal ion by forming metal ion chelates with COS. Moreover, the physicochemical properties and antioxidant properties of nIon2+-COS/SA gel films were systematically characterized and evaluated by in vitro experiments. The gel films showed good antibacterial activity against Gram-negative and Gram-positive bacteria. In addition, the gel films showed good cytocompatibility in cellular experiments, and the gel films with Zn2+ and Sr2+ addition significantly accelerated wound healing in whole skin defect model experiments. Therefore, this nIon2+-COS/SA gel film is an ideal candidate material for wound dressing.

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Screening antioxidant capacity of Mexican maize (Zea mays L.) landraces with colored grain using ABTS, DPPH and FRAP methods

Cited by 6 publications

References 30 publications

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice

COLOR Y CARACTERÍSTICAS NUTRACÉUTICAS DE TORTILLAS DE MAÍCES (Zea mays L.) NATIVOS PREPARADAS CON DIFERENTES DOSIS DE ÁLCALI Y REFRIGERADAS

Multifunctional Gel Films of Marine Polysaccharides Cross-Linked with Poly-Metal Ions for Wound Healing

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