Role of Tripodal DNA Modified Gold Nanoparticles in Colorimetric Aptasensing

Kou, Xinyue; Liu, Tao; Yang, Xiaobo; Tang, Yuguo; Gong, Xiao

doi:10.1016/j.colcom.2017.11.001

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…Gold nanoparticles (AuNPs) are the most commonly applied nanomaterials for colorimetric biosensors with the unique property of localized surface plasmon resonance . In this contribution, we have developed two novel AuNPs-based logic gates for multiple miRNAs analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Gold nanoparticles (AuNPs) are the most commonly applied nanomaterials for colorimetric biosensors with the unique property of localized surface plasmon resonance. 23 which are demonstrated as the biomarkers of nonsmall cell lung cancer. 24−26 The key to realize AuNPs-based colorimetric assays is the control of nanoparticle dispersion and aggregation, which can be achieved by cross-linking aggregation (CA) and non-cross-linking aggregation (NCA).…”

Section: ■ Introductionmentioning

confidence: 99%

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Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs

Chen

Tang

et al. 2019

ACS Appl. Mater. Interfaces

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Abnormal miRNA expressions are closely related to the occurrence and development of cancers. It is of great significance to monitor miRNA expression levels for early diagnosis and therapy of the diseases. This study presents two independent colorimetric strategies for simultaneously monitoring multiple miRNAs based on cross-linking or non-cross-linking aggregations of gold nanoparticles (AuNPs). By introducing a Y shaped DNA structure and two types of DNA modified AuNPs, a triple-input DNA AND logic gate is facilely developed with the cross-linking aggregation of AuNPs as the signal output. To improve the sensitivity and shorten reaction time, the logic gate is modified by further employing a three DNA strands formed duplex and hybridization chain reaction. Non-cross-linking aggregation of AuNPs is used to evaluate the concentration of initial miRNA inputs. This strategy does not require DNA modification of AuNPs and ultrahigh sensitivity is achieved with the amplification of hybridization chain reaction. The present work may provide powerful tools for multiple miRNAs diagnostics and inspire further development of DNA based logic gates.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs

Chen

Tang

et al. 2019

ACS Appl. Mater. Interfaces

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“…Aptamers are single-stranded DNA or RNA oligonucleotides, which show high binding affinity and specificity to certain target molecules, including small molecules, proteins, and even cells. − A 15-mer single-stranded DNA (5′-GGTTGGTGTGGTTGG-3′) was previously selected to recognize thrombin with secondary structure transformation . Due to their high affinity, a number of aptasensors for thrombin assay have been developed. , For example, Gosaia et al fabricated a label-free detection system using an aptamer-functionalized nanoporous membrane and achieved a limit of detection (LOD) as low as 10 pM .…”

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confidence: 99%

Fabrication of Polymeric Ferrocene Nanoparticles for Electrochemical Aptasensing of Protein with Target-Catalyzed Hairpin Assembly

Chai

Cheng

et al. 2019

Anal. Chem.

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Herein we describe a novel isothermal and enzyme-free electrochemical aptasensor for protein detection via the employment of polymeric ferrocene nanoparticles (PFcNPs) and target-catalyzed hairpin assembly amplification. The synthesized PFcNPs not only load numerous Fc molecules for enhanced electrochemical output but also possess plenty of amino groups, which increase the water solubility and facilitate the conjugation with the aptamer toward the recognition of target protein. After the formation of an aptamer/protein complex, the conformation of the DNA probe changes, which further triggers hairpin assembly on top of DNA tetrahedral structures modified on the electrode interface. The process can be recycled, and multiple PFcNPs are localized on the electrode. Thus, an amplified electrochemical signal is able to be recorded, which is sufficient to achieve a demonstrated limit of detection as low as 67 fM. This developed aptasensor can also discriminate target protein from other interfering substances with a high selectivity. Furthermore, it has been successfully applied in diluted real blood serum samples. All of these features make the present methodology a promising candidate for ultratrace protein biosensors.

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Recent advances in optical aptasensor technology for amplification strategies in cancer diagnostics

et al. 2020

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Role of Tripodal DNA Modified Gold Nanoparticles in Colorimetric Aptasensing

Cited by 8 publications

References 22 publications

Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs

Triple-Input Molecular AND Logic Gates for Sensitive Detection of Multiple miRNAs

Fabrication of Polymeric Ferrocene Nanoparticles for Electrochemical Aptasensing of Protein with Target-Catalyzed Hairpin Assembly

Recent advances in optical aptasensor technology for amplification strategies in cancer diagnostics

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