Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering

Peközer, Görke Gürel; Ramazanoğlu, Mustafa; Schlegel, Karl Andreas; Kök, Fatma Neşe; Köse, Gamze Torun

doi:10.1080/21691401.2017.1332637

Cited by 18 publications

(19 citation statements)

References 31 publications

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“…The same CCK-8 kit was used to evaluate cell proliferation at 1, 4, 7 and 14 days [41]. The cell proliferative rate was determined via the absorbance at OD 450 nm using a microplate reader (SpectraMax M5, Molecular Devices, Sunnyvale, CA, USA).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

“…At 4, 7 and 14 days, the cells were lysed and assayed for ALP activity using a Wako ALP kit (Wako Pure Chemical, Osaka, Japan) [41]. ALP activity (n ¼ 6) was normalized by the total protein from each sample, which was quantified using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) [41].…”

Section: Alp Activity Assaymentioning

confidence: 99%

“…Alizarin red S (ARS) staining for mineralization by the cells After 7, 14 and 21 days [41], the cell-scaffold constructs were washed with PBS, fixed in 10% formaldehyde, and staining with 2% (mass/volume) ARS (Millipore, Billerica, MA, USA). Quantification of cell mineralization was performed by measuring the absorbance at 550 nm after eluting the ARS deposit with 10% (mass/volume) cetylpyridinium chloride (Sigma-Aldrich, St. Louis, MO, USA) [43].…”

Section: Quantitative Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%

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Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Xia

Chen

Zhang

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Literature search revealed no systematic report on iron oxide nanoparticle-incorporating calcium phosphate cement scaffolds (IONP-CPC). The objectives of this study were to: (1) use γFeO nanoparticles (γIONPs) and αFeO nanoparticles (αIONPs) to develop novel IONP-CPC scaffolds, and (2) investigate human dental pulp stem cells (hDPSCs) seeding on IONP-CPC for bone tissue engineering for the first time. IONP-CPC scaffolds were fabricated. Physiochemical properties of IONP-CPC scaffolds were characterized. hDPSC seeding on scaffolds, cell proliferation, osteogenic differentiation and bone matrix mineral synthesis by cells were measured. Our data demonstrated that the osteogenic differentiation of hDPSCs was markedly enhanced via IONP incorporation into CPC. Substantial increases (about three folds) in ALP activity and osteogenic gene expressions were achieved over those without IONPs. Bone matrix mineral synthesis by the cells was increased by two- to three folds over that without IONPs. The enhanced cellular osteogenesis was attributed to: (1) the surface nanotopography of IONP-CPC scaffold, and (2) the cell internalization of IONPs released from IONP-CPC scaffold. Our results demonstrate that the novel CPC functionalized with IONPs is promising to promote osteoinduction and bone regeneration. In conclusion, it is highly promising to incorporate γIONPs and αIONPs into CPC scaffold for bone tissue engineering, yielding substantially better stem cell attachment, spreading and osteogenic differentiation, and much greater bone mineral synthesis by the seeded cells. Therefore, novel CPC scaffolds containing γIONPs and αIONPs are promising for dental, craniofacial and orthopaedic applications to substantially enhance bone regeneration.

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Section: Cell Proliferation Assaymentioning

confidence: 99%

Section: Alp Activity Assaymentioning

confidence: 99%

Section: Quantitative Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%

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Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Xia

Chen

Zhang

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…Then tibiae were ashed at 800 °C for 4 h, weighed again and dissolved in 1 mL 6N HCl. Calcium content (mg/dl) was obtaining by colorimetric determination with Quantichrom calcium Assay kit [38] (BioAssay Systems, Hayward, CA).…”

Section: Anthropometric and Compositional Analysis Of The Tibia (Calcmentioning

confidence: 99%

Effects of C-peptide replacement therapy on bone microarchitecture parameters in streptozotocin-diabetic rats

Maurotti

Russo

Musolino

et al. 2020

Preprint

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Pathological pathways involved in the development of diabetic complications are still unclear.Several studies suggested a pathogenic role of C-peptide deficiency in vascular and neuropathic complications. However, to date, the role of C-peptide on diabetes-related bone loss has not been investigated. The objective of this study was to test the effects of a 6-week regimen of rat C-peptide infusion on bone by combining micro-CT imaging with histological testing.Twenty-three, four-month-old male Wistar rats were randomly divided into three groups: Normal control group; Sham diabetic control group; Diabetic plus C-peptide group. Diabetes was induced by injection of streptozotocin. Rat C-peptide was delivered via subcutaneously osmopumps. We assessed several trabecular microarchitectural parameters and cellular and matrix proteins in bone tissue sections.At the end of the study, both the normal control and diabetic plus C-peptide groups had a higher tibia weight than the diabetic control group (p = 0.05 and p = 0.02, respectively). C-peptide levels significantly and positively correlated with the trabecular thickness (r= 0.45, p=0.08) and negatively with structure model index (r= -0.40, p=0.09). Furthermore, it positively correlated with one of the histological assessments (i.e. PLIN1 score; p=0.02). Micro-CT evaluation showed significant intergroup differences in trabecular thickness (p=0.02), trabecular space (p = 0.05) and cross-sectional thickness (p=0.05). Diabetic plus C-peptide group showed a higher trabecular thickness (p<0.001), cross-sectional thickness (p=0.03) and a lower trabecular space (p=0.05) than the normal control group. Both the normal control and diabetic plus C-peptide groups had more Runx-2 and PLIN1 positive cells in comparison to the diabetic control group (p = 0.045 and p = 0.034).For the first time we demonstrated that the diabetic rats receiving rat C-peptide had higher quality of trabecular bone, than diabetic rats not receiving it. C-peptide could have a role in the prevention of diabetes-related bone loss.

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“…To date, stem cell research has meant a tremendous advance for cell therapy and tissue engineering (Alizadeh et al 2016; Bai et al 2016; Gao et al 2016; Guo et al 2017; Zhang et al 2016). Embryonic stem cells and adult type stem cells are current sources of stem cells(Gurel Pekozer et al 2018; Kariminekoo et al 2016; Mohammadian et al 2016; Momenzadeh et al 2017). However, given the ethical and technical problems, the use of embryonic stem cells may have obvious drawbacks, such as limited availability, complicated culture system and tumorigenicity (Blum and Benvenisty 2008; Gruen and Grabel 2006).…”

Section: Introductionmentioning

confidence: 99%

Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

Wang

et al. 2018

Cell Tissue Bank

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Amnion, which is usually discarded as medical waste, is considered as abundant sources for mesenchymal stem cells. In human and veterinary medicine, the multipotency of mesenchymal stem cells derived from amnion (AMSCs) together with their plasticity, self-renewal, low immunogenicity and nontumorigenicity characteristics make AMSCs a promising candidate cell for cell-based therapies and tissue engineering. However, up till now, the multipotential characteristics and therapeutic potential of AMSCs on preclinical studies remain uncertain. In this work, we successfully obtained AMSCs from Beijing duck embryos in vitro, and also attempted to detect their biological characteristics. The isolated AMSCs were phenotypically identified, the growth kinetics and karyotype were tested. Also, the cells were positive for MSCs-related markers (CD29, CD71, CD105, CD166, Vimentin and Fibronection), while the expression of CD34 and CD45 were undetectable. Additionally, AMSCs also expressed the pluripotent marker gene OCT4. Particularly, when appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. Together, these results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine.

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Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering

Cited by 18 publications

References 31 publications

Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Injectable calcium phosphate scaffold with iron oxide nanoparticles to enhance osteogenesis via dental pulp stem cells

Effects of C-peptide replacement therapy on bone microarchitecture parameters in streptozotocin-diabetic rats

Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

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