Multilineage potential research of Beijing duck amniotic mesenchymal stem cells

Abstract

Amnion, which is usually discarded as medical waste, is considered as abundant sources for mesenchymal stem cells. In human and veterinary medicine, the multipotency of mesenchymal stem cells derived from amnion (AMSCs) together with their plasticity, self-renewal, low immunogenicity and nontumorigenicity characteristics make AMSCs a promising candidate cell for cell-based therapies and tissue engineering. However, up till now, the multipotential characteristics and therapeutic potential of AMSCs on preclinical studies remain uncertain. In this work, we successfully obtained AMSCs from Beijing duck embryos in vitro, and also attempted to detect their biological characteristics. The isolated AMSCs were phenotypically identified, the growth kinetics and karyotype were tested. Also, the cells were positive for MSCs-related markers (CD29, CD71, CD105, CD166, Vimentin and Fibronection), while the expression of CD34 and CD45 were undetectable. Additionally, AMSCs also expressed the pluripotent marker gene OCT4. Particularly, when appropriately induced, AMSCs could be induced to trans-differentiate into adipocytes, osteoblasts, chondrocytes and neurocytes in vitro. Together, these results demonstrated that the isolated AMSCs maintained their stemness and proliferation in vitro, which may be useful for future cell therapy in regenerative medicine.

Mesenchymal stromal cells isolated from chicken peripheral blood secrete bioactive factors with antimicrobial and regenerative properties

“…Far less is known about MSCs isolated from birds. Only a few groups have reported on the isolation of MSCs from turkeys and ducks (41)(42)(43), and although more research has been done studying the characteristics of MSCs derived from chickens (44), the effects of chicken MSCs, in particular the chicken MSC secretome, on wound healing has not been explored.…”

<p>Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells</p>

“…Mesenchymal stem cells (MSCs) are characterized by a multipotent cell lineage with a high capacity of differentiation into different cell types, which can execute trophic, paracrine, and immunomodulatory functions in other cells based on the microenvironment. 1,2 In regenerative medicine, MSCs derived from fetal annexes such as amniotic fluid, amniotic membrane, and umbilical cord blood and vessels have previously been isolated and well-characterized in humans 3 and other species, such as dogs, [4][5][6][7] cats, 8,9 bovines, 10 sheep, 11,12 horses, 13 rats, 14 rabbits, 15 and ducks, 16 suggesting the advantages of using it as raw material for the creation of cell banks. [17][18][19][20] Immunomodulatory activities occur through direct contact between the MSCs and tissues or through paracrine interaction mediated by interferon-gamma (IFN-γ), produced by the body's immune cells, which act on natural killer cells, monocytes, neutrophils, and macrophages.…”