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Biodegradable synthetic scaffolds hold great promise for oral and craniofacial guided tissue regeneration and bone regeneration. To overcome the limitations of current scaffold materials in terms of osteogenic and antimicrobial properties, we have developed a novel silver-modified/ collagen-coated electrospun PLGA/PCL scaffold (PP-pDA-Ag-COL) with improved antimicrobial and osteogenic properties. Our novel scaffold was generated by electrospinning a basic PLGA/PCL matrix, followed by silver nanoparticle (AgNPs) impregnation via in situ reduction and polydopamine coating, and then coated by collagen I. The three intermediate materials involved in the fabrication of our scaffolds, PLGA/PCL (PP), PLGA/PCL-polydopamine (PP-pDA), and PLGA/PCL-polydopamine-Ag (PP-pDA-Ag) were used as control scaffolds. Scanning electron micrographs and mechanical testing indicated that the unique 3-dimensional structures with randomly-oriented nanofibrous electrospun-scaffold architectures, the elasticity modulus and the tensile strength were maintained after modifications. CCK-8 cell proliferation analysis demonstrated that the PP-pDA-Ag-COL scaffold was associated with higher MC3T3 proliferation rates than the three control scaffolds employed. Scanning electron and fluorescence light microscopy illustrated that PP-pDA-Ag-COL scaffolds significantly enhanced MC3T3 cell

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