Role of dietary lipids and butylated hydroxy anisol (BHA) administration on albino rats. Part II. Liver and heart unsaturated fatty acids

Abstract

Adult male albino rats were supplemented with edible plant oils, corn maize and cotton seed oils and their semihydrogenated forms (Heliopolis and Sultan cooking fat). In general, the supplementation of tested oils produced increasing effect on the levels of total unsaturated fatty acids (UFA), C18:1, C18:2 and C20:4 UFA of liver and heart of rats. Liver content of UFA showed very highly significant increases in the levels of C18:1 and C18:2 in all groups of rats fed with tested oils accompanied with non significant changes in total UFA and C20:4 levels. Heart content of UFA showed increases in the levels of C18:1 and C18:2 and insignificant changes observed in total UFA and C20:4, except, in rats fed with sultan cooking fat, showed a significant increase in the level of total UFA. The level of increase in C18:1 and C18:2 was very highly significant in case of rats fed corn maize oil and Sultan cooking fat, and significant in case of rats fed cotton seed oil and Heliopolis cooking fat. Also, liver and heart UFA contents were significantly increased after antioxidant (Butylated hydroxy anisol, BHA) administration. Addition of BHA to tested diets abolished or minimized fatty infiltration produced by dietary fatty diet in the liver cells.

Fatty Acid Composition of Serum, Adipose Tissue, and Liver in Rats Fed Diets Containing Corn Oil or Cottonseed Oil

“…In previous studies, such as that by Abu-el-Zahab et al [8], who compared the effect of CO versus that of CSO on hepatic fatty acid composition, data were presented for a limited number of fatty acids (C18:1n-9 + C18:2n-6 and C20:4n-6), whereas values for 11 fatty acids are reported here. In the present study, the use of CSO rather than CO was associated with changes in fatty acid composition of serum, adipose tissue, and liver, with changes being noted for SFAs, MUFAs, and n-3 PUFAs, but not n-6 PUFAs.…”

“…In one, in which the fatty acid composition of adipose tissue was determined in rats, the level of C18:1n-9 was found to be lower in animals fed CSO than in ones fed CO, but the level of C18:2 n-6 was found to be higher [5]. In the other, where the fatty acid composition of liver and heart was determined in rats, there was no effect of diet on hepatic or cardiac levels of either C18:1n-9 + C18:2n-6 or C20:4n-6 [8]. In all three studies on the effect of dietary CSO on fatty acid composition, no data were presented for either palmitic acid (C16:0), the third most abundant fatty acid (after C18:2 n-6 and C18:1n-9) in both oils, or the n-3 fatty acids (such as α-linolenic [C18:3n-3]), which is found at a much lower level in CSO than CO [7].…”

“…The effect of diet on serum levels of other fatty acids was not determined. There have been only two studies that have compared the effect of CSO with that of another vegetable oil on tissue fatty acid composition [5,8]. In both studies, the dietary oil of comparison was CO.…”