No abstract

Adult male albino rats were supplemented with edible plant oils, corn maize and cotton seed oils and their semihydrogenated forms (Heliopolis and Sultan cooking fat). In general, the supplementation of tested oils produced increasing effect on the levels of total unsaturated fatty acids (UFA), C18:1, C18:2 and C20:4 UFA of liver and heart of rats. Liver content of UFA showed very highly significant increases in the levels of C18:1 and C18:2 in all groups of rats fed with tested oils accompanied with non significant changes in total UFA and C20:4 levels. Heart content of UFA showed increases in the levels of C18:1 and C18:2 and insignificant changes observed in total UFA and C20:4, except, in rats fed with sultan cooking fat, showed a significant increase in the level of total UFA. The level of increase in C18:1 and C18:2 was very highly significant in case of rats fed corn maize oil and Sultan cooking fat, and significant in case of rats fed cotton seed oil and Heliopolis cooking fat. Also, liver and heart UFA contents were significantly increased after antioxidant (Butylated hydroxy anisol, BHA) administration. Addition of BHA to tested diets abolished or minimized fatty infiltration produced by dietary fatty diet in the liver cells.

SummaryAdult male albino rats were supplemented with edible plant oils, corn maize and cotton seed oils and their semihydrogenated forms (Heliopolis and Sultan cooking fats) for a period of 3 months. In general, the supplementation of tested oils produced increasing effect on the serum levels of total C18 : 1, C18 : 2 and C20 : 4 unsaturated fatty acids (UFA). The increases were significant in case of feeding corn maize oil and Sultan cooking fat for total and CIS : I and C18 : 2 UFA. However, the degree of significance was intensified by the feeding of cotton seed oil and Heliopolis cooking fat. Supplementation of Sultan cooking fat showed a significant increase in serum level of C20 : 4, while the other tested oils and Heliopolis cooking fat produced very highly significant increase.Butylated hydroxy anisol (BHA) produced no effect on the level of UFA when administered with dietary tested edible lipid but its role revealed by maintaining the higher level of UFA due to administration of oils and its semihydrogenated analogous.

Background: This study is used to compare the effects between three different INSUGEAST preparations (preps) (recombinant insulin from yeast cells) in management of insulin dependent diabetes mellitus (IDDM) and its complications. The preps were extracted from modified genetically saccharomyces cerviciae on culture pure camel milk (PC), partial pure camel milk (PPC) and specific media (SP) parallel with insulin stander (ST). Also the study extended to evaluate the difference in effect of the aforementioned INSUGEAST preps in three forms, regular, biphasic and recombinant in yeast cells against the stability and severity of hyperglycemia. Method: IDDM was induced in female albino rats by intraperitoneal injection (i.p) of a single dose 70mg/kg/B.wt of streptozotocin (STZ) in citrate buffer. The diabetic rats were classified into two groups; the 1 st group divided into 5 equal subgroups, four subgroups, each was injected subcutaneously (s.c) by PC, PPC, SP and St respectively, in the biphasic form, in a dose of 4.2 IU/kg/B.wt for 3weeks.The 5 th subgroup was considered as the diabetic control (STZ), in addition to 6 normal rats was used as free control group (C). The 2 nd group divided as the same as in 1 st group but the INSUGEAST preps used involve little protamine zinc or in the regular form in addition to other 2 subgroups, each of them treated orally either with an amount of yeast (Y) containing insulin equivalent to 4.2 IU/kg/B.wt or added to it specific additive (YA) and treated for the same period. Blood glucose level either fasting (FBG) or after 2h from glucose and insulin loading were measured weekly for all groups. Biochemical assays to ascertain diabetic complications resemble in hepatorenal dysfunction included: aminotransferase aspartate (AST) and alanate (ALT), alkaline phosphatase (ALP), creatinine (Creat) and blood urea nitrogen (BUN); hyperlipidemia indicators included: total cholesterol (T-Ch), low density lipoprotein cholesterol (LDL-Ch), high density lipoprotein cholesterol (HDL-Ch) and triglycerides (TG) were measured at the end of the experimental schedule. The result of this study showed that: 1-Blood glucose level in 1 st group was markedly decreased after insulin injection in all different preps and similar to the effect of insulin standard. 2-The hepato-renal dysfunction and hyperlipidemea induced by STZ injection, showed significant improvements after INSUGEAST preps (biphasic form), especially with insulin prepared on PPC and PC media. 3-INSUGEAST preps on the 2 nd group (regular form, in the yeast) decrease the level of FBG significantly only at 1 st week of treatment, their reduction effect decrease, the severity of hyperglycemia Bull. Egypt. Soc. Physiol. Sci. 31 (2) 2011 Elsisi & Elgamal 104 increase, animals could not survive and mortality rate increase 4-The diabetic complications (hepato-renal dysfunction, hyperlipidemia) increased in all groups of this part and not showed any improvements. 5-Histopathological study made on pancreas, liver and kidney tissues conform the present ...