Role of cultivation media in the development of yeast strains for large scale industrial use

Abstract

The composition of cultivation media in relation to strain development for industrial application is reviewed. Heterologous protein production and pentose utilization by Saccharomyces cerevisiae are used to illustrate the influence of media composition at different stages of strain construction and strain development. The effects of complex, defined and industrial media are compared. Auxotrophic strains and strain stability are discussed. Media for heterologous protein production and for bulk bio-commodity production are summarized.

Glucoamylase by recombinant Kluyveromyces lactis cells: production and modelling of a fed batch bioreactor

“…There are two reasons why K. lactis JA6 GAA was allowed to grow in a rich complex non-selective medium. First, microorganisms generally grow more vigorously in rich media than in synthetic media because rich media already contain biosynthetic precursors to be channelled into anabolic pathways with a significant effect on growth and production [21]. Second, no loss of plasmids was observed during 72 h of growth in YEP lactose, as it resulted by comparing the number of colony forming units (CFU) after incubation, at 30°C, of agar plates containing either selective or non-selective medium.…”

Cultivation of Methylotrophs

“…The principal interest of methylotrophic yeasts resides in the potential for exploiting large cultivation to yield 'biocommodities' from inexpensive substrates, including synthesis of heterologous protein, enzymes (e.g. α-amylase), and amino acids [40,119,120]. Typically 10-90 g methanol l À1 was used in laboratory-scale experiments, using bioreactors of 3-150 l capacity, such as the 3 litre Braun CT2-2 bioreactor (Note 8).…”

Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

“…Specifically, casamino acid allowed for complete use of the carbon source and, consequently, enhanced cell growth and heterologous protein production (Merico et al 2004). In addition, cultivation medium is composed of casamino acid, supplementing free amino acid requirements for heterologous protein production in recombinant yeast and relieving the metabolic burden of cells (Hahn-Hagerdal et al 2005).…”

“…In contrast, recombinant Xyn2 activity attained in the YPD medium was the lowest at 40.85 U/ml, but this medium yielded the highest biomass concentration at 5.19 g/l. In yeast extract-containing mediums, such as YPD, sugars and lactate serve as auxiliary carbon sources and strongly affect yeast cultivation rates (Hahn-Hagerdal et al 2005). Variable selections for the Plackett-Burman design were based on these media that produced the highest levels of recombinant Xyn2, regardless of biomass concentrations.…”

“…Despite the importance of the genetic background, the composition of cultivation media is crucial to the successful development of strains for large-scale industrial production of heterologous proteins (Hahn-Hagerdal et al 2005). A cultivation medium is designed to reflect the biosynthetic capacity of microorganisms, which consequently affects strain analyses and performance in industrial applications.…”