Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

Fuzi, Siti Fatimah Zaharah Mohamad; Mahadi, Nor Muhammad; Jahim, Jamaliah Md; Murad, Abdul Munir Abd.; Bakar, Farah Diba Abu; Jusoh, Mazura; Rahman, Roshanida A.; Illias, Rosli Md.

doi:10.1007/s13213-011-0258-x

Cited by 14 publications

(4 citation statements)

References 46 publications

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“…Xylanases are effectively applied in various industrial applications, including feed, food, textile, pulp, and paper. Xylanases have additionally been suggested to provide valuable possibilities for the bioconversion and biodegradation of diverse agro-industrial and processing wastes into numerous value-added products [ 104 ]. However, the presence of XIs can have a negative effect on several industrial processes in which adding microbial xylanases improves the quality of the final product [ 65 ].…”

Section: Strategies To Reduce the Effect Of Xis In Food Processing An...mentioning

confidence: 99%

Xylanase Inhibitors: Defense Players in Plant Immunity with Implications in Agro-Industrial Processing

Tundo

Mandalà

Sella

et al. 2022

IJMS

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Xylanase inhibitors (XIs) are plant cell wall proteins largely distributed in monocots that inhibit the hemicellulose degrading activity of microbial xylanases. XIs have been classified into three classes with different structures and inhibition specificities, namely Triticum aestivum xylanase inhibitors (TAXI), xylanase inhibitor proteins (XIP), and thaumatin-like xylanase inhibitors (TLXI). Their involvement in plant defense has been established by several reports. Additionally, these inhibitors have considerable economic relevance because they interfere with the activity of xylanases applied in several agro-industrial processes. Previous reviews highlighted the structural and biochemical properties of XIs and hypothesized their role in plant defense. Here, we aimed to update the information on the genomic organization of XI encoding genes, the inhibition properties of XIs against microbial xylanases, and the structural properties of xylanase-XI interaction. We also deepened the knowledge of XI regulation mechanisms in planta and their involvement in plant defense. Finally, we reported the recently studied strategies to reduce the negative impact of XIs in agro-industrial processes and mentioned their allergenicity potential.

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Section: Strategies To Reduce the Effect Of Xis In Food Processing An...mentioning

confidence: 99%

Xylanase Inhibitors: Defense Players in Plant Immunity with Implications in Agro-Industrial Processing

Tundo

Mandalà

Sella

et al. 2022

IJMS

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“…Xylanases (EC 3.2.1.8) have played an important role in many industrial processes and have been applied as additives to enhance the quality of baked goods, animal feeds as well as to bleach kraft pulp [1]. The xylanases are also the crucial enzymes for the depolymerization of xylan by randomly hydrolyzing the β-1,4-glycosidic bonds to produce xylooligosaccharides with different lengths, thus they have great potential commercial value.…”

Section: Introductionmentioning

confidence: 99%

A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization

Shi

Zhang

et al. 2013

Biotechnol Biofuels

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BackgroundXylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent attention as powerful enzyme as well as in the field of biomass utilization.ResultsA xylanase gene (xyn10A) with 3,474 bp was cloned from the extremely thermophilic bacterium Thermotoga thermarum that encodes a protein containing 1,158 amino acid residues. Based on amino acid sequence homology, hydrophobic cluster and three dimensional structure analyses, it was attested that the xylanase belongs to the glycoside hydrolase (GH) families 10 with five carbohydrate binding domains. When the xylanase gene was cloned and expressed in Escherichia coli BL21 (DE3), the specific enzyme activity of xylanase produced by the recombinant strain was up to 145.8 U mg-1. The xylanase was optimally active at 95°C, pH 7.0. In addition, it exhibited high thermostability over broad range of pH 4.0-8.5 and temperature 55-90°C upon the addition of 5 mM Ca2+. Confirmed by Ion Chromatography System (ICS) analysis, the end products of the hydrolysis of beechwood xylan were xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose.ConclusionsThe xylanase from T. thermarum is one of the hyperthermophilic xylanases that exhibits high thermostability, and thus, is a suitable candidate for generating XOs from cellulosic materials such as agricultural and forestry residues for the uses as prebiotics and precursors for further preparation of furfural and other chemicals.

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“…In control samples, peak xylanase activity was measured at 5545 U L −1 on the 2nd day of incubation. In this study, it is demonstrated that the optimal productivity of the xylanase enzyme was achieved at shorter incubation times (12 h), which, as per Fuzi et al (2011), renders the medium suitable for use when seeking efficient and time-saving production.…”

Section: Medium Optimizationmentioning

confidence: 88%

Bioengineered xylanase from Misiones Argentina rainforest: A bakery enhancement approach

Molina,

Cazzaniga,

Sgroppo

et al. 2024

Journal of Food Science

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In this study, we pursued the heterologous expression of the xylanase gene from Trichoderma atroviride, a native fungus in the province of Misiones, and used it to enhance the textural properties of baked goods through varying enzymatic concentrations. This marks the inaugural exploration into its functionality in the context of bread production. The recombinant xylanase exhibited improved activity, reaching 36,292 U L−1, achieved by supplementing the culture medium with dextrose. Following the optimization of recombinant xylanase concentration, promising results emerged, notably reducing hardness and chewiness parameters of bread significantly. Our findings underscore the potential of this native fungal enzyme for industrial processes, offering a sustainable and efficient means to enhance the quality of baked goods with broad implications for the food industry. No prior research has been documented on the heterologous expression of the xylanase gene derived from T. atroviride, from the Misiones rainforest, expressed in Kluyveromyces lactis.Practical ApplicationThis research, focusing on the isolation and cloning of xylanase enzyme from Trichoderma atroviride, a native fungus in the province of Misiones, offers a valuable tool for improving the texture of bakery products. By optimizing enzyme concentrations, our findings present a practical approach for the food industry, offering a viable solution to improve the overall quality and consumer satisfaction of bakery products.

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Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

Cited by 14 publications

References 46 publications

Xylanase Inhibitors: Defense Players in Plant Immunity with Implications in Agro-Industrial Processing

Xylanase Inhibitors: Defense Players in Plant Immunity with Implications in Agro-Industrial Processing

A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization

Bioengineered xylanase from Misiones Argentina rainforest: A bakery enhancement approach

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