Riset Sitotoksik Campuran Ekstrak Daun Sirsak (Annona muricata L) dan Kulit Buah Manggis (Garcinia mangostana L) pada Sel Vero dan AML12

Mardja, Tuty Erlina; Rahmi, Fitria; Rusmawati, Eka; Adriany, Rina; Murtiningsih, Murtiningsih; Setijanti, Herlina B.; Usia, Tepy

doi:10.25026/jtpc.v3i4.116

Cited by 4 publications

(5 citation statements)

References 2 publications

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“…Positively charged amino groups in chitosan that interact with negatively charged cell membranes will also form a layer that is capable of inhibiting ion channel transformation and inhibiting the work of enzymes in cells, so that cells will lack any nutrients and may contribute to cell death. 15 Haruan fish scale chitosan contains antioxidants that may reduce the activity of free radicals such as hydrogen peroxide, superoxide anions, and Cu2+ ions by binding to free radical ions. Antioxidants in chitosan have two types of mechanisms in inhibiting the oxidation process or stopping the reaction of free radicals.…”

Section: Discussionmentioning

confidence: 99%

“…17,18 The results of the research carried out by Saputra and Kroesnadi (2020) found that chitosan was not toxic to BHK-21 fibroblast cells. 15 Chitosan toxicity may be determined by identifying the degree of deacetylation, a degree of more than 35% will indicate low toxicity, whereas if the degree of deacetylation is below 35%, the toxicity depends on the dose of chitosan used. In regards to SNI, the value of the deacetylation degree of chitosan is 75%, while the chitosan in haruan fish scales has a degree of deacetylation of 85.25%, which indicates low toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…12, 13 The toxicity test parameter is referred to as the Inhibitory Concentration 50 (IC50) value, which is defined as the concentration value that inhibits growth in cells by 50% of the cell population and proves the potential toxicity of a compound to cells.. 14 Toxicity categories for natural materials proposed by Balantyne (1999) in Mardja et al (2016) can be seen in the following table. 15…”

Section: Introductionmentioning

confidence: 99%

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THE TOXICITY TEST OF Channa striata SCALE CHITOSAN ON BHK-21 FIBROBLAST CELLS IN VITRO

Fanshuri¹,

Putri²,

Azizah³

et al. 2022

Dentino

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Background:Regarding to the data generated by Basic Health Research in 2018, the prevalence of Indonesian people with dental and oral problems was amounted to 57.6%. Haruan fish scale chitosan has been shown to be capable of inhibiting growth and killing microorganisms that cause oral disease and has the potential to become a safe biomaterial. Toxicity testing was carried out to ensure safety of the chemical compounds before they can be used as drugs or during clinical trials. Purpose:This research was intended to analyze the toxicity of haruan fish scales (Channa striata) chitosan on BHK-21 fibroblast cells by utilizing the MTT assay method. Method:This research was classified as a true experimental research by adopting a post-test-only design with a control group design consisting of 6 groups. The treatment of haruan fish scale chitosan was given to 4 groups with concentrations of 25%, 50%, 75%, and 100%, with 2 control groups, specifically cell control and media control. The absorbance value was read by means of ELISA reader and the percentage of cell viability was subsequently calculated. The results of the average percentage of cell viability were then analyzed using SPSS to obtain the IC50 value. Result:The calculation of the average cell viability of each concentration was successfully obtained in this research. The average cell viability at 25% concentration was 2.106%, at 50% concentration was 12.01%, at 75% concentration was 77%, and at 100% concentration was 80.194%. The average percentage of cell viability data was further entered into the SPSS software and the value of IC50> 1000 µg/mL was successfully obtained. Conclusion: Haruan fish chitosan (Channa striata) is not toxic to BHK-21 fibroblas cell. Keywords : Channa striata , Fibroblast cell, Scale chitosan, Toxicity test.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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THE TOXICITY TEST OF Channa striata SCALE CHITOSAN ON BHK-21 FIBROBLAST CELLS IN VITRO

Fanshuri¹,

Putri²,

Azizah³

et al. 2022

Dentino

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“…With regard to the cytotoxicity of natural ingredients, in cases where IC 50 > 1000μg/mL, they are classified as non-toxic. 18 The final cytotoxicity test result provided information about the percentage of cells which had been able to survive. 15 This data proved that ethanol extract of Binjai leaves administered by MTT assay method was not cytotoxic to Vero cells.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity test of binjai leaf (Mangifera caesia) ethanol extract in relation to Vero cells

Dwidhanti¹,

Taufiqurrahman²,

Sukmana³

2018

Dent. J.

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Background: Binjai leaves (Mangifera caesia) constitute one part of a medicinal plant from South Borneo that contains potential anticancer and antioxidant flavonoids. Before using medicinal plants as adjuvant therapy material, a cytotoxicity test of a material extract needs to be conducted in order to establish the safety of natural ingredients that will be used in the production of medicinal products. Purpose: This research aimed to determine whether the ethanol extract of binjai leaves proved cytotoxic to Vero cells and determine the value of IC50 after the administering of ethanol extract of Binjai leaves by means of an MTT assay method. Methods: This research incorporated a true experimental method with posttest-only control design that consisted of ten groups. The Binjai leaf ethanol extract of varying concentrations was administered to eight groups, namely;1.25µg/mL, 62.5µg/mL, 125µg/mL, 250µg/mL, 500µg/mL, 1000µg/mL, 2000µg/mL and 4000µg/mL. The control groups consisted of two groups, one cell control group and one media control group. The cell viability percentage was calculated by an absorbent of ELISA reader. Results: The probit analysis result had an IC50 value of 2498.48µg/mL (IC50>1000µg/mL constituted a non-toxic category). Conclusion: Ethanol extract of Binjai leaves is not cytotoxic to Vero cells as shown by an assay MTT method which produced an IC50 value of 2498.48µg/mL.

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“…Moreover, these cells are highly stable, sensitive, and easy to culture. 14 The toxic effect of an extract is usually reported as the half maximal inhibitory concentration (IC50) which indicates the presence or absence of toxic potential of the extract. 15 The in vivo toxicity test is a procedure used to assess toxic effects of an extract on test laboratory animals by administering specific doses.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Nephrotoxicity Evaluation of the Methanol Extract of Ficus deltoidea Jack Leaf

2024

TJNPR

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Ficus deltoidea Jack leaves extract (FDLE) is rich in phytochemicals like alkaloids, phenolics, flavonoids, and steroids. These phytochemicals possess an excellent potential for medicinal applications. This study aimed to assess the kidney toxicity of FDLE through in vitro and in vivo methods. FDLE was obtained by maceration in methanol at room temperature for 24 h. The in vitro toxicity of FDLE at 10 -100 µg/mL was evaluated in Baby Hamster Kidney (BHK)-21fibroblast cells using the MTT assay method. The in vivo toxicity was assessed by histopathological examination of the kidneys of Wistar rats following oral acute administration of FDLE at 1.250 mg/kg, 2.500 mg/kg, and 3.750 mg/kg twice daily for 14 days. In vitro toxicity tests showed no toxic effect of FDLE on BHK-21 fibroblast cells. The percentage cell viability of BHK-21 fibroblast cells was higher than 60% at all the concentration of FDLE tested, and the IC50 value was 71.63 µg/mL. The histopathological analysis revealed no significant difference in the haemarrage and necrosis observed in the tissues of the kidneys of rats in the FDLE treated groups compared to the control group. Therefore, it could be concluded that FDLE demonstrated no toxic effects in vitro and in vivo, and may be relatively safe when administered to humans.

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Riset Sitotoksik Campuran Ekstrak Daun Sirsak (Annona muricata L) dan Kulit Buah Manggis (Garcinia mangostana L) pada Sel Vero dan AML12

Cited by 4 publications

References 2 publications

THE TOXICITY TEST OF Channa striata SCALE CHITOSAN ON BHK-21 FIBROBLAST CELLS IN VITRO

THE TOXICITY TEST OF Channa striata SCALE CHITOSAN ON BHK-21 FIBROBLAST CELLS IN VITRO

Cytotoxicity test of binjai leaf (Mangifera caesia) ethanol extract in relation to Vero cells

In Vitro and In Vivo Nephrotoxicity Evaluation of the Methanol Extract of Ficus deltoidea Jack Leaf

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