Ridge preservation using an in situ hardening biphasic calcium phosphate (β-TCP/HA) bone graft substitute—a clinical, radiological, and histological study

Kakar, Ashish; Rao, B H Sripathi; Hegde, Shashikanth; Deshpande, Nikhil; Lindner, Annette; Nagursky, Heiner; Patney, Aditya; Mahajan, Harsh

doi:10.1186/s40729-017-0086-2

Cited by 31 publications

(21 citation statements)

References 21 publications

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“…Bio‐safe and absorbable bioceramics, such as beta‐tricalcium phosphate (β‐TCP), have been clinically applied to bone graft materials (Kakar et al, 2017; Zijderveld, Zerbo, van den Bergh, Schulten, & ten Bruggenkate, 2005). β‐TCP grafts possess the properties of bioresorption in the body, unlike hydroxyapatite grafts, and continuous Ca and P ion supplying ability to stimulate bone formation (Oh, Ko, Jaiswal, & Whang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

et al. 2020

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Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl‐glycyl‐aspartic acid (RGD)‐rich motif to enhance cell behavior and is anticipated as a xeno‐free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta‐tricalcium phosphate fine particles (RCP/β‐TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β‐TCP. Recombinant peptide was thermal crosslinked and freeze‐dried to prepare RCP. An aqueous dispersion of β‐TCP fine particles was added to RCP to obtain RCP/β‐TCP. Subsequently, RCP/β‐TCP were characterized using scanning electron microscopy (SEM), energy dispersive X‐ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β‐TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β‐TCP, β‐TCP particles dose‐dependently covered the surface of RCP. Cell culture tests showed that RCP/β‐TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3‐E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β‐TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β‐TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.

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Section: Introductionmentioning

confidence: 99%

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

et al. 2020

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“…Only limited data exists in terms of alloplastic bone substitute materials as investigated in the present study (Valdivia-Gandur et al, 2016, Wildburger et al, 2017, Favero et al, 2016. Some volumetric data were published (Naenni et al, 2017), but only limited histologic data on alloplastic materials used for ridge preservation (Kakar et al, 2017). However, data on the preclinical use of non-resorbable (ePTFE)-membranes compared to a resorbable alloplastic membrane showed comparable results (Al Salamah et al, 2012).…”

Section: Introductionmentioning

confidence: 76%

Histologic analyses of flapless ridge preservation in sockets with buccal dehiscence defects using two alloplastic bone graft substitutes

et al. 2019

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OBJECTIVES To investigate whether one of two synthetic bone substitute materials used for ridge preservation in the extraction sockets with buccal dehiscence defects was superior regarding new bone formation and ridge preservation and to compare it to sites left for spontaneous healing. MATERIALS AND METHODS In sixteen dogs, P3 and P4 were hemi-sectioned and the respective distal roots were extracted. Following the preparation of a mucoperiosteal flap without vertical releasing incisions, 50% of the buccal bone was carefully removed. The extraction sites were randomly assigned either to a ridge preservation procedure (alloplastic bone substitute material (two test groups)) or to spontaneous healing (control group). Descriptive histology and histomorphometric analyses were performed at healing times of 4, 8, and 16 weeks. In case of homogeneous variances, the results were analyzed by one-way ANOVA, followed by Tukey's post-hoc test. If inhomogeneous, the data was analyzed using Welch-type ANOVA, followed by the Games-Howell post-hoc test. RESULTS The use of bone substitute material led to significantly greater horizontal dimensions amounting to 3.3 mm (SD = 0.67; test 1) and 3.5 mm (SD = 0.72; test 2) compared to spontaneous healing (1.7 mm, SD = 0.23) at 16 weeks of healing (p < 0.0001). A significant difference was observed between spontaneous healing and the test groups in terms of newly formed bone tissue at 4, 8, and 16 weeks (p = 0.001), with values reaching 7.9, 21.8, and 36.8% (test 1), 5.0, 10.4, and 29% (test 2), and 26.2, 43.5, and 56.4% (control), but there were no significant differences between the test groups (p > 0.05). The final ridge profile was more favorable after ridge preservation (p < 0.001) as demonstrated by a loss of 28.8% (spontaneous healing) and an increase in both test groups at 16 weeks (test 1 = 60.5% and test 2 = 31.2%). CONCLUSIONS The use of alloplastic materials rendered greater horizontal dimensions and a more favorable maintenance of the ridge profile. CLINICAL RELEVANCE Alloplastic bone substitute materials can successfully be used for ridge preservation procedures.

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“…The objective of another recent article was to evaluate an in situ hardening biphasic hydroxyapatite/b-TCP bone graft substitute for ridge preservation after tooth extraction without primary wound closure or a barrier membrane. Histomorphometric analysis of core biopsy samples showed that using this substitute results in an effective preservation of the ridge contour and sufficient new bone formation [18]. A systematic review analyzed the outcomes of randomized controlled trials of socket grafting procedures performed with flapless extraction of teeth after a minimum healing period of 12 week.…”

Section: Discussionmentioning

confidence: 99%

Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

et al. 2020

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Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

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Ridge preservation using an in situ hardening biphasic calcium phosphate (β-TCP/HA) bone graft substitute—a clinical, radiological, and histological study

Cited by 31 publications

References 21 publications

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

Bone forming ability of recombinant human collagen peptide granules applied with β‐tricalcium phosphate fine particles

Histologic analyses of flapless ridge preservation in sockets with buccal dehiscence defects using two alloplastic bone graft substitutes

Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

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