Abstract-This study investigated the effect of different storage conditions of cold-pressed rice bran oil from 2 different regions in Thailand (Lopburi province, LRBO and Yasothorn province, YRBO). All samples were stored at different temperature as opened and sealed bottle for 12 months. Fatty acid composition by nuclear magnetic resonance, NMR spectroscopy, chemical properties such as acid value, iodine value and peroxide value were reported. Also, the antioxidant content and its activity were evaluated, including FRAP assay (Ferric reducing antioxidant potential) and DPPH radical scavenging assay (1, 1-diphenyl-2-picrylhydrazyl radical reducing power methods). Cold-pressed rice bran oils sealed bottles for 12 months and freshly opened bottles have shown no affect to acid value, iodine value and peroxide value (p<0.05). Fatty acid composition of oil by 1 H NMR technique obtained a good agreement with gas chromatography technique and observed mol% of unsaturated fatty acid was slightly decreased after year storage. After 12-month storage below 5°C temperature, LRBO and YRBO showed good radical scavenging activities compared to quercetin with DPPH IC 50 of 0.647±0.02 and 0.663±0.02 mg/mL and with FRAP assay of 0.42106± 0.002 and 0.37471± 0.002 g quercetin equivalents/g, respectively.