Review on the use of enzymes for the detection of organochlorine, organophosphate and carbamate pesticides in the environment

Dyk, J. Susan van; Pletschke, Brett I.

doi:10.1016/j.chemosphere.2010.10.033

Cited by 356 publications

(147 citation statements)

References 165 publications

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“…Strawberry fruit is particularly prone to negative effects of the fungicides, as C. nymphaeae usually infects mature fruits. An additional problem is the pathogenic resistance induced by the frequent use of specific fungicides [2]. Their use is also limited due to a lack of pesticides registered for this crop and restrictions imposed on their usage.…”

Section: Introductionmentioning

confidence: 99%

Alternative products against anthracnose affect selected primary and secondary metabolites in strawberry fruit

Weber¹,

Veberič²,

Reščič³

et al. 2016

Fruits

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-Introduction. Anthracnose disease causes severe economic losses in strawberry (Fragaria × ananassa) production. Plant response to the pathogen attack is closely linked to its content and composition of polyphenols. In order to reduce the damage caused by pathogens, organic and inorganic fungicides are applied. The aim was to determine the influence of selected fungicides on various metabolites in strawberry fruit. Materials and methods. The efficiency of potassium bicarbonate (PBC) and acetic acid (AA) was compared with the control and a fungicide treatment in the strawberry cv. Elsanta. To examine the possible effects of these fungicides, artificial inoculation was performed in all treatments except for the control. The percentage of infected fruits and the content of selected primary and secondary metabolites in non-infected and infected fruits were determined. Results and discussion. Both analyzed inorganic fungicides proved effective against anthracnose. AA treated fruits were the least infected (only 4%), followed by PBC, fungicide and control. The highest percentage (9.26%) of infected fruits was determined following artificial infection treatment. Total sugar content decreased for 32% in infected control and AA treated fruits. PBC treatment exhibited a positive effect on the organic acid content in comparison with other treatments. The content of phenolic compounds was higher in PBC treated fruits and did not change significantly after infection. Infection increased the total flavonol content of the fruit. Conclusion. The use of PBC and AA potentially present a prospective protection in the strawberry orchards especially for controlling anthracnose in organic fruit crops.Keywords: strawberry / Fragaria × ananassa / anthracnose (Colletotrichum spp.) / alternative fungicides / biological control / integrated disease management / phenolics Résumé -Des produits alternatifs contre l'anthracnose affectent certains métabolites primaires et secondaires de la fraise. Introduction. L'anthracnose provoque des pertes économiques importantes en production de fraises (Fragaria × ananassa). La réponse de la plante à l'attaque des agents pathogènes est étroitement liée à sa teneur et à sa composition en polyphénols. Pour réduire les dommages causés par les agents pathogènes, des fongicides organiques et inorganiques sont appliqués. Nous avons cherché à déterminer l'influence de ces fongicides sur certains métabolites présents dans la fraise. Matériel et méthodes. L'efficacité du bicarbonate de potassium (PBC) et de l'acide acétique (AA) a été comparée à celle du contrôle et d'un traitement fongicide sur le cultivar Elsanta. En vue d'examiner les effets potentiels de ces fongicides, une inoculation artificielle a été réalisée pour tous les traitements sauf pour le contrôle. Le pourcentage de fruits infectés, ainsi que le contenu en certains métabolites primaires et secondaires contenus dans les fruits non infectés et infectés ont été déterminés. Résultats et discussion. Les deux fongicides inorganiques étudiés s...

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Section: Introductionmentioning

confidence: 99%

Alternative products against anthracnose affect selected primary and secondary metabolites in strawberry fruit

Weber¹,

Veberič²,

Reščič³

et al. 2016

Fruits

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“…Exhaustive reviews on enzyme inhibition-based biosensors, including inhibition determination in organic phase are provided by Amine et al (2006) and López et al (2006). The application of various enzymes: acetylcholinesterase, acid phosphatase, alkaline phosphatise, organophosphorus hydrolase, and tyrosinase for the quantification of OP pesticides in the environment is extensively revised by Van Dyk et al (2011). Another group of electrochemical biosensors for OP pesticides analysis is that of the microbial sensors.…”

Section: Biosensors Based Methods For Op Pesticides Analysismentioning

confidence: 99%

Organophosphorus Pesticides Analysis

Stoytcheva¹,

Zlatev²

2011

Pesticides in the Modern World - Trends in Pesticides Analysis

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“…Firstly it was demonstrated that the detoxification of organophosphate pesticides by genetically engineered microorganisms and the genes encoding these hydrolases have been cloned and expressed in Streptomyces lividans, Yarrowia lipolytica P. pseudoalcaligenes, E. coli, and Pichia pastoris (Wu et al, 2004;Fu et al, 2004;Shen et al, 2009). The site-directed mutagenesis has been used to improve the substrate specificity and stereo selectivity of OPH (Casey et al, 2011;Van Dyk and Brett, 2011). To generate OPH variants with up to 25-fold improvements in hydrolysis of methyl parathion, directed evolution have recently been used (Cho et al, 2002).…”

Section: Fungi: Aspergillus Nigermentioning

confidence: 99%