Leaf removal at véraison and Double Maturation Raisonnée improved the phenolic composition of wine, producing a full-bodied wine. On the other hand, early leaf removal significantly augmented the yield and titratable acidity, hydroxycinnamic acids and flavanols of wine, which might have led to a fresher but less-bodied wine. © 2016 Society of Chemical Industry.
-Introduction. Anthracnose disease causes severe economic losses in strawberry (Fragaria × ananassa) production. Plant response to the pathogen attack is closely linked to its content and composition of polyphenols. In order to reduce the damage caused by pathogens, organic and inorganic fungicides are applied. The aim was to determine the influence of selected fungicides on various metabolites in strawberry fruit. Materials and methods. The efficiency of potassium bicarbonate (PBC) and acetic acid (AA) was compared with the control and a fungicide treatment in the strawberry cv. Elsanta. To examine the possible effects of these fungicides, artificial inoculation was performed in all treatments except for the control. The percentage of infected fruits and the content of selected primary and secondary metabolites in non-infected and infected fruits were determined. Results and discussion. Both analyzed inorganic fungicides proved effective against anthracnose. AA treated fruits were the least infected (only 4%), followed by PBC, fungicide and control. The highest percentage (9.26%) of infected fruits was determined following artificial infection treatment. Total sugar content decreased for 32% in infected control and AA treated fruits. PBC treatment exhibited a positive effect on the organic acid content in comparison with other treatments. The content of phenolic compounds was higher in PBC treated fruits and did not change significantly after infection. Infection increased the total flavonol content of the fruit. Conclusion. The use of PBC and AA potentially present a prospective protection in the strawberry orchards especially for controlling anthracnose in organic fruit crops.Keywords: strawberry / Fragaria × ananassa / anthracnose (Colletotrichum spp.) / alternative fungicides / biological control / integrated disease management / phenolics Résumé -Des produits alternatifs contre l'anthracnose affectent certains métabolites primaires et secondaires de la fraise. Introduction. L'anthracnose provoque des pertes économiques importantes en production de fraises (Fragaria × ananassa). La réponse de la plante à l'attaque des agents pathogènes est étroitement liée à sa teneur et à sa composition en polyphénols. Pour réduire les dommages causés par les agents pathogènes, des fongicides organiques et inorganiques sont appliqués. Nous avons cherché à déterminer l'influence de ces fongicides sur certains métabolites présents dans la fraise. Matériel et méthodes. L'efficacité du bicarbonate de potassium (PBC) et de l'acide acétique (AA) a été comparée à celle du contrôle et d'un traitement fongicide sur le cultivar Elsanta. En vue d'examiner les effets potentiels de ces fongicides, une inoculation artificielle a été réalisée pour tous les traitements sauf pour le contrôle. Le pourcentage de fruits infectés, ainsi que le contenu en certains métabolites primaires et secondaires contenus dans les fruits non infectés et infectés ont été déterminés. Résultats et discussion. Les deux fongicides inorganiques étudiés s...
IntroductionGrape quality is reflected in a balanced composition of primary and secondary metabolites, which constitute different parts of the fresh berries during their development and ripening. The accumulation of these metabolites within the berries is affected by genetic characteristics, cultivation practices, and climate conditions (Jackson and Lombard, 1993).The phenolic compounds in the skins of red berry grapevine varieties are mostly anthocyanins, hydroxycinnamic acids, stilbenoids, flavanols, and flavonols, which are also the building blocks of the grapes' and wines' sensorial properties (Jackson and Lombard, 1993;Gonzalez-Alvarez et al., 2013). In red grapes, anthocyanins are responsible for the red color of the skins and consequently define the color and quality of the must and wine (Liang et al., 2008). Other polyphenols, such as flavonols and flavanols, mostly contribute to wine flavor and astringency (Li et al., 2009). Phenolic compounds are biosynthesized through the phenylpropanoid and flavonoid pathways and originate from the amino acid precursor, phenylalanine. These pathways are among the most studied and thoroughly described secondary metabolic pathways in plants (Yang et al., 2012). Polyphenolic compounds play an important role in the sensorial quality of grapes and wines. Their transformation during the vinification process directly or indirectly influences the quality of the wine: its structure and sensorial properties (Cheynier, 2005;Noguerol-Pato et al., 2013).In order to change the composition of fresh berries and improve the quality of the wines produced from these, different canopy techniques, especially training systems, bunch thinning, defoliation, dehydration etc., have been introduced in viticulture ( Kyraleou et al., 2015;Liu et al., 2015). Grape dehydration, used for the production of late harvest, botrytized, and ice wines, represents a measure applied for the production of sweeter wines in general, in which the contents of soluble solids, phenolics, and aroma
<p><strong>Aim</strong>: Two different yield reductions based on cluster thinning (CT) were performed to determine their impact on vine growth, yield, and grape and wine composition of 'Blauer Portugieser' grapevine variety.</p><p><strong>Methods and results</strong>: Two levels of cluster thinning (limited CT1 – 20-30 % and severe CT2 – 40-50 % cluster reduction) were applied at the pea-size berry (BBCH 75) phenological stage in 2007, 2008 and 2011. The potential impact of CT was determined by measurements of vine growth and fertility potential, berry weight, berry colour, soluble solids content, titratable acidity, pH and total phenolics. Additionally, for the first time, individual phenolic compounds were identified and quantified in berry skin and wine by HPLC-MS. In general, CT of 'Blauer Portugieser' significantly decreased titratable acidity in grape and wine, and increased pH and chromatic parameters in grape and alcohol content and volatile acidity in wine. A significant decrease in yield per vine (of 0.92 kg of grape/vine), together with an increase in soluble solids (of 2.8 °Brix) in grape and pH and total extract content in wine was only observed in severe CT (CT2). Furthermore, CT2 significantly increased the content of total anthocyanins, flavonols and hydroxycinnamic acids, but not total flavanols, in grape and wine. CT2 significantly increased the content and proportion of <em>p</em>-coumaroyl pentose in grape and wine, catechin in grape, epicatechin in wine, quercetin-3-glucuronide (the main flavonol in 'Blauer Portugieser') in grape and wine, the content of myricetin-3-glucoside in grape, and the content of 3-glucosides of laricitrin, myricetin and quercetin in wine. Finally, CT2 increased the content and the proportion of 3-glucosides of delphinidin, petunidin and peonidin but decreased the proportion of malvidin-3-glucoside in grape and wine.</p><p><strong>Conclusion</strong>: A significant impact on yield and grape and wine composition was observed, particularly in the CT2 treatment, in which the yield loss was compensated by higher contents of soluble solids in grape, alcohol in wine, and phenolic compounds in grape and wine.</p><p><strong>Significance and impact of the study</strong>: The present study is the first report on the impact of different levels of cluster thinning on yield and grape and wine composition of 'Blauer Portugieser' variety. Grape and wine composition has been evaluated with an emphasis on a detailed profile of individual and total phenolic contents. The results are undoubtedly useful for winegrowers, who, until now, lacked technological guidelines to optimize 'Blauer Portugieser' yield and wine quality.</p>
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