Restriction fragment length polymorphism in amplification products of the bovine <i>PIT1</i> gene and assignment of <i>PIT1</i> to bovine chromosome 1

Moody, D. E.; Pomp, Daniel; Barendse, W.

doi:10.1111/j.1365-2052.1995.tb02620.x

Cited by 40 publications

(31 citation statements)

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“…The HinfI (-) allele was the less frequent in the two genetic groups (Table 1). The predominance of the HinfI (+) allele was also detected in different European cattle breeds (Moody et al, 1995;Sabour et al, 1996;Klauzinska et al, 2000;Oprzadek et al, 2003), in Gir and crossbred dairy cattle (Mattos, 2000), and in Gir × Holstein crosses (Silva et al, 2006).…”

Section: Allele Frequenciesmentioning

confidence: 98%

“…they may reflect the evolutionary history of this mutation if the HinfI (+) allele is considered as the ancestral allele that underwent a rare case of mutation. This hypothesis seems more appropriate, since the HinfI (+) allele is prevalent in most bovine breeds (Moody et al, 1995;Mattos, 2000).…”

Section: Pit1 Effects On Production Traitsmentioning

confidence: 99%

“…Polimerase Chain Reaction (PCR) with the PIT1 specific primers 5'-CAATGAGAAAGTTGGTGC-3' and 5'-TCTGCATTCGAGATGCTC-3' was analyzed according to Moody et al (1995). Amplified products were digested with the restriction enzyme HinfI and fragments were separated in 3% agarose gel, stained with 0.45 µg mL -1 ethidium bromide.…”

Section: Pcr-rflp Analysismentioning

confidence: 99%

“…Among the genes involved in mammalian growth, PIT1 is considered as a candidate. PIT1 was mapped to Bos taurus chromosome 1 (Moody et al, 1995), and belongs to a group of genes that code for proteins involved in animal development. The specific pituitary transcription factor Pit-1, coded by the PIT1 gene, is required for the initiation of the expression of the growth hormone releasing factor gene (Lin et al, 1992) and is involved in the maintenance of the expression of the gene coding for the β subunit of thyroid stimulating hormone (Lin et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Association of PIT1 genotypes with growth traits in Canchim cattle

Carrijo

Alencar

Toral³

et al. 2008

Sci. agric. (Piracicaba, Braz.)

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Use of genetic markers can aid on the identification of animals with highest breeding values in beef cattle. The PIT1 gene codes for the Pit-1 transcription factor is essential for the activation of prolactin, growth hormone and PIT1 genes. This research is an investigation of the effect of PIT1-HinfI polymorphism on growth traits of 509 Canchim animals, from two lineages, GG1 and GG2. PIT1 genotypes were identified through PCR-RFLP. Genotype effect on phenotypic values for birth weight (BW), standardized weaning weight (W240), weight at 12 months of age (W365), and the average daily weight gain from birth to weaning (AGBW), and from weaning to 12 months of age (AGW12) were analyzed by the least squares method. Effects of the interaction between the animal's genetic group and PIT1 genotype for W240, AGBW and AGW12 were observed (P < 0.05). Differences between means of HinfI (-/-), HinfI (+/+) and HinfI (+/-) genotypes for W240 and AGBW were observed in GG2 (P < 0.05), revealing superiority of (-/-) genotype for those traits. Means for genotypes (+/+) and (+/-) for W240 and AGBW, did not differ from one another, suggesting a dominance effect of the HinfI (+) allele. The HinfI (-) allele had a favorable effect on W240 and AGBW in GG2, when present in homozygosis. The difference between PIT1 behavior observed in the two genetic groups may suggest the action of a quantitative trait locus linked to PIT1, segregating only in GG2 population. Key words: RFLP, bovine, pituitary factor 1, candidate gene ASSOCIAÇÃO DE GENÓTIPOS PIT1 COM CARACTERÍSTICAS DE CRESCIMENTO EM GADO CANCHIMRESUMO: O uso de marcadores genéticos pode favorecer a identificação de animais geneticamente superiores em bovino de corte. O gene PIT1 codifica para o fator de transcrição Pit-1, crítico para ativar a expressão dos genes da prolactina, hormônio de crescimento e PIT1. Esta pesquisa investiga o efeito do polimorfismo HinfI-PIT1 sobre características de crescimento em 509 animais da raça Canchim, pertencentes à duas linhagens, GG1 e GG2. Os genótipos do gene PIT1 foram identificados pela técnica PCR-RFLP. Os efeitos dos genótipos sobre valores fenotípicos para pesos ao nascimento (PN), pesos padronizados à desmama (P240) e ao ano (P365), ganhos médios diários de peso do nascimento à desmama (GMND), e da desmama aos 12 meses de idade (GMD12) foram analisados pelo método dos quadrados mínimos. Foram observados efeitos de interação entre grupo genético do animal e genótipo de PIT1 sobre P240, GMND e GMD12 (P < 0.05). Diferenças entre médias dos genótipos HinfI (-/-), HinfI (+/+) e HinfI (+/-) para P240 e GMND foram observadas em GG2 (P < 0,05), revelando superioridade do genótipo HinfI (-/-). As médias dos genótipos (+/+) e (+/-) para P240 e GMND, não diferiram, sugerindo efeito de dominância do alelo HinfI (+). O alelo HinfI (-) mostrou-se favorável sobre P240 e GMND em GG2, quando em homozigose. A diferença de comportamento de PIT1 observada nos dois grupos genéticos pode sugerir a ação de loco de característica quantitativa ligado ao ge...

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Section: Allele Frequenciesmentioning

confidence: 98%

Section: Pit1 Effects On Production Traitsmentioning

confidence: 99%

Section: Pcr-rflp Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Association of PIT1 genotypes with growth traits in Canchim cattle

Carrijo

Alencar

Toral³

et al. 2008

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

show abstract

“…Besides, Pit-1 gene is responsively involved in protein and hormone syntheses as well as differentiation and proliferation of pituitary cells (Zhang et al, 2009). In genomic DNA of cattle, Pit-1 resides in 1q21-q22 region of chromosome 1 (Woolard et al, 1994) which is flanked by two microsatellites DNA of TGLA57 and RM95 yielding a total of 129 amino acids consisting of 6 exons distantly spaced by 5 introns (Moody et al, 1995). Restriction fragment length polymorphism (RFLP) provided first evidence of high polymorphism in this gene at Hinf-1 restriction sites, located at 5 and exon 6 (Woolard et al, 1994).…”

Section: Introductionmentioning

confidence: 99%