A cattle genetic linkage map was constructed which covers more than 95 percent of the bovine genome at medium density. Seven hundred and forty six DNA polymorphisms were genotyped in cattle families which comprise 347 individuals in full sibling pedigrees. Seven hundred and three of the loci are linked to at least one other locus. All linkage groups are assigned to chromosomes, and all are orientated with regards to the centromere. There is little overall difference in the lengths of the bull and cow linkage maps although there are individual differences between maps of chromosomes. One hundred and sixty polymorphisms are in or near genes, and the resultant genome-wide comparative analyses indicate that while there is greater conservation of synteny between cattle and humans compared with mice, the conservation of gene order between cattle and humans is much less than would be expected from the conservation of synteny. This map provides a basis for high-resolution mapping of the bovine genome with physical resources such as Yeast and Bacterial Artificial Chromosomes as well as providing the underpinning for the interpolation of information from the Human Genome Project.

Egg production and egg quality are complex sex-limited traits that may benefit from the implementation of marker-assisted selection. The primary objective of the current study was to identify quantitative trait loci (QTL) associated with egg traits, egg production, and body weight in a chicken resource population. Layer (White Leghorn hens) and broiler (Cobb-Cobb roosters) lines were crossed to generate an F2 population of 508 hens over seven hatches. Phenotypes for 29 traits (weekly body weight from hatch to 6 weeks, egg traits including egg, albumen, yolk, and shell weight, shell thickness, shell puncture score, percentage of shell, and egg shell colour at 35 and 55 weeks of age, as well as egg production between 16 and 55 weeks of age) were measured in hens of the resource population. Genotypes of 120 microsatellite markers on 28 autosomal groups were determined, and interval mapping was conducted to identify putative QTL. Eleven QTL tests representing two regions on chromosomes 2 and 4 surpassed the 5% genome-wise significance threshold. These QTL influenced egg colour, egg and albumen weight, percent shell, body weight, and egg production. The chromosome 4 QTL region is consistent with multiple QTL studies that define chromosome 4 as a critical region significantly associated with a variety of traits across multiple resource populations. An additional 64 QTL tests surpassed the 5% chromosome-wise significance threshold.

Three populations of Hereford cattle differing in inbreeding levels and genetic potential for growth were genotyped for seven DNA polymorphisms. The populations were compared to determine differences in allele frequency and genetic variation. Significant differences in allele frequency among the populations were found at six of the seven polymorphisms genotyped, and average genetic variation differed as expected when inbreeding levels were considered. Effects of several polymorphisms on growth and maternal EPD were evaluated for one population (Miles City Line 1 Herefords) using regression analysis. Substitution of a B allele for an A allele of the kappa-casein polymorphism accounted for significant decreases in direct birth weight and maternal 180-d gain from birth to weaning EPD explaining 15% and 8%, respectively, of EPD variability. Several other significant effects accounting for small portions of EPD variability were also detected.

SummaryMouse populations differing in metabolic rate have been developed through selection for high (MH) and low (ML) heat loss (HLOSS), along with randomly selected controls (MC). Objectives of this study were to (a) compare MH, ML and MC lines for HLOSS and correlated traits of feed intake, body composition and organ weights ; (b) compare three widely used inbred mouse lines with MH, ML and MC for the same traits ; and (c) investigate potential genotype by diet interaction resulting from feeding diets differing in fat percentage. Heat loss (kcal\day) of MH and ML mice differed by 37 % of the mean and remained significant (33 %) when HLOSS was expressed on a fat-free mass basis. MH mice consumed more energy than ML with a greater difference in mice fed high-fat compared with standard diets (27 % vs 13n9 %). Despite greater energy consumption, MH mice were leaner than ML with a difference in total body fat percentage of 40 %. The greatest difference in HLOSS between selection and inbred lines was between MH and C57BL\6J (BL), which differed by 26n3 %. MH and BL mice also differed in energy intake (15n5 %). Body composition of BL mice was similar to MH when fed a standard diet, but similar to ML when fed a high-fat diet. Crosses between MH and ML or between MH and BL would be useful to investigate the genetic regulation of, and identify quantitative trait loci influencing HLOSS, energy intake and body composition. Feeding of a high-fat diet may allow diet-specific loci influencing body composition to be identified in MH and BL lines.

-Bone fractures at the end of lay are a significant problem in egg-laying strains of hens. The objective of the current study was to identify quantitative trait loci (QTL) associated with bone mineralization and strength in a chicken resource population. Layer (White Leghorn hens) and broiler (Cobb-Cobb roosters) lines were crossed to generate an F2 population of 508 hens over seven hatches, and 26 traits related to bone integrity, including bone mineral density (BMD) and content (BMC), were measured. Genotypes of 120 microsatellite markers on 28 autosomal groups were determined, and interval mapping was conducted to identify QTL regions. Twenty-three tests representing three chromosomal regions (chromosomes 4, 10 and 27) contained significant QTL that surpassed the 5% genome-wise threshold, and 47 tests representing 15 chromosomes identified suggestive QTL that surpassed the 5% chromosome-wise threshold. Although no significant QTL influencing BMD and BMC were detected after adjusting for variation in body weight and egg production, multiple suggestive QTL were found. These results support previous experiments demonstrating an important genetic regulation of bone strength in chickens, but suggest the regulation may be due to the effects of multiple genes that each account for relatively small amounts of variation in bone strength.bone mineral density / chickens / QTL / osteoporosis

Dual-energy X-ray absorptiometry can be used as a noninvasive tool to monitor the skeletal integrity of live birds. A pDexa X-ray bone densitometer was used to determine bone mineral densities (BMD) of the left tibia together with the fibula and the humerus of live, unanesthetized birds. Densitometry effectively detected changes in bone integrity of live birds fed varying levels of dietary calcium. Hens consuming 1.8, 3.6, or 5.4% dietary calcium had BMD of 0.147, 0.157, and 0.176 g/cm2 (SEM = 0.005), respectively (linear effect, P < 0.001). Likewise, bone ash weight, breaking force, stress, modulus of elasticity, and eggshell traits also increased linearly in response to increased calcium in the diet (P < 0.05). Densitometric live scans for BMD were positively correlated (P < 0.001) with bone breaking force (r = 0.65) and bone ash (r = 0.77). We also monitored BMD in live Leghorn and broiler females during their life cycle. The tibial BMD of White Leghorns and broilers increased from 15 to 65 wk of age with the BMD of the broiler tibia increasing at a greater rate than that of the Leghorn tibia (line x age interaction, P < 0.0001). A precipitous drop in BMD occurred during an induced molt of Leghorns subjected to 10 d of feed withdrawal. Our long-term goal is to improve skeletal integrity in egg-type chickens by genetic selection for improved BMD. By crossing a broiler with an egg-laying line, an F2 resource population of birds has been developed to identify quantitative trait loci influencing BMD in chickens.

Background: The callipyge mutation is located within an imprinted gene cluster on ovine chromosome 18. The callipyge trait exhibits polar overdominant inheritance due to the fact that only heterozygotes inheriting a mutant paternal allele (paternal heterozygotes) have a phenotype of muscle hypertrophy, reduced fat and a more compact skeleton. The mutation is a single A to G transition in an intergenic region that results in the increased expression of several genes within the imprinted cluster without changing their parent-of-origin allele-specific expression.

A polymorphism was identified in the bovine PIT1 gene by digesting polymerase chain reaction (PCR) products with the restriction enzyme Hinff. This polymorphism was segregating in five diverse breeds of cattle. PIT1 was sublocalized to the centromeric region of bovine chromosome 1 by linkage analysis.