Restricted replication of mouse adenovirus strain FL in mouse Balb/c cells transformed by Simian Adenovirus 7

Dussai, Elisabeth; Faucon-Biguet, Nicole; Samolyk, D; Boni, Claudette; Tournier, P

doi:10.1016/0042-6822(82)90156-8

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…For all practical purposes HeLa cells represent a nonpermissive host for AdFl virus. Our data thus agree with previous observations concerning the limited host range of AdFl (Larsen and Nathans, 1977;Dussaix et al, 1982). The small amount of virus formed after prolonged incubations of HeLa cells certainly does not lead to major morphological changes of the cells and infected HeLa cells can be kept alive for at least 3 weeks.…”

Section: Discussionsupporting

confidence: 83%

Defective expression of mouse adenovirus Fl in human cells.

Antoine¹,

Aleström²,

Schilling³

et al. 1982

The EMBO Journal

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HeLa cells infected with mouse adenovirus strain Fl (AdFl) produce at least 2000 times less virus than permissive mouse 3T3 cells. Viral DNA synthesis, however, proceeds unimpaired. The defect in virion production was linked to a dramatic reduction in the synthesis of AdFI structural proteins, in particular the hexon. The identity of the AdFl hexon gene product was recognized through its immunogenic reactivity towards an antiserum raised against the human adenovirus type 2 (Ad2) hexon gene product. This crossreactivity is reflected in an extensive DNA sequence homology between AdFI and Ad2 DNA at position 53-60, the locus of the hexon gene, on the Ad2 physical map. Through hybridization at different formamide concentrations, the present study identifies one additional, highly conserved region at map positions 14-15 on the Ad2 genome.

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Section: Discussionsupporting

confidence: 83%

Defective expression of mouse adenovirus Fl in human cells.

Antoine¹,

Aleström²,

Schilling³

et al. 1982

The EMBO Journal

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“…In the regions corresponding to El, MAV-1 DNA and amino acid sequences are more similar to those of the nononcogenic or weakly oncogenic adenoviruses Ad4, 5, and 7 than to that of the highly oncogenic adenovirus Adl2. This conclusion is consistent with observations that MAV-1 is nononcogenic (12).…”

Section: Discussionsupporting

confidence: 93%

“…One standard criterion for the orientation of adenoviruses is to position the transforming region, early region 1 (El), at the left end of the genome (2). There is no evidence that MAV-1 is oncogenic in vivo (12), thus the criterion of a transforming region does not orient MAV-1. However, proteins encoded in El of adeno-* Corresponding author.…”

mentioning

confidence: 99%

Identification of mouse adenovirus type 1 early region 1: DNA sequence and a conserved transactivating function

Ball

Williams

Spindler

1988

J Virol

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The left end of the genome of mouse adenovirus type 1 (also known as strain FL) was characterized by determination of the DNA sequence, amino acid similarities with early region proteins of primate adenoviruses, and a functional assay. Several specific DNA sequence features were similar to those found in human adenoviruses, and open reading frames from this region could encode proteins similar to human adenovirus early region lA and early region 1B proteins. DNAs from this region were tested in transient-expression assays in human and mouse cells and were found to transactivate the human adenovirus type 5 early region 3 promoter fused to the chloramphenicol acetyltransferase gene. The data indicate structural and functional homologies between mouse adenovirus type 1 early region 1 and early region 1 of primate adenoviruses.

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