Defective expression of mouse adenovirus Fl in human cells.

Abstract

HeLa cells infected with mouse adenovirus strain Fl (AdFl) produce at least 2000 times less virus than permissive mouse 3T3 cells. Viral DNA synthesis, however, proceeds unimpaired. The defect in virion production was linked to a dramatic reduction in the synthesis of AdFI structural proteins, in particular the hexon. The identity of the AdFl hexon gene product was recognized through its immunogenic reactivity towards an antiserum raised against the human adenovirus type 2 (Ad2) hexon gene product. This crossreactivity is reflected in an extensive DNA sequence homology between AdFI and Ad2 DNA at position 53-60, the locus of the hexon gene, on the Ad2 physical map. Through hybridization at different formamide concentrations, the present study identifies one additional, highly conserved region at map positions 14-15 on the Ad2 genome.

A size analysis of the adenovirus replicon.

“…As pointed out above, this line of reasoning ignores the presence of different terminal proteins on these templates. We have previously shown that Ad2 and AdFl DNA show very little homology (Antoine et al, 1982). It is thus to be expected that the primary sequences of these two proteins, which, as demonstrated here, are distinguished by a size difference of -8 kd, are very different from each other.…”

“…We have previously shown that Ad2 and AdFl DNA show very little homology (Antoine et al, 1982). In the respective heterologous systems, they are totally inactive.…”

“…AdFl only grows to very low titers in HeLa cells but replicates its DNA very efficiently in this system (Antoine et al, 1982). AdFl only grows to very low titers in HeLa cells but replicates its DNA very efficiently in this system (Antoine et al, 1982).…”

“…The observed selectivity of the nuclear extracts for their respective templates was also observed in a nuclear extract obtained from AdFl-infected HeLa cells. AdFl only grows to very low titers in HeLa cells but replicates its DNA very efficiently in this system (Antoine et al, 1982). Since, in contrast to mouse 3T3 cells, HeLa cells can be grown in suspension cultures, AdFl-infected HeLa cells represent a very convenient system for the study of AdFl DNA replication.…”

“…The origin of both HeLa and 3T3 cells has been described previously (Antoine et al, 1982). Infections of mouse 3T3 cells and of HeLa cells with mouse AdFl were performed under conditions discussed by Antoine et al (1982).…”