Genome organization of mouse adenovirus type 1 early region 1: A novel transcription map

Ball, Amy Oberhauser; Beard, Clayton W.; Redick, Sambra D.; Spindler, Katherine R.

doi:10.1016/0042-6822(89)90444-3

Cited by 34 publications

(25 citation statements)

References 60 publications

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“…Unlike human adenovirus, MAV-1 only produces a single 200-amino-acid E1A protein (4,53). Although MAV-1 E1A is not an essential gene for viral replication in cell culture (61), it is a virulence gene in both outbred and inbred mice (52,55), emphasizing the importance of MAV-1 E1A for viral pathogenesis in vivo.…”

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confidence: 99%

Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1

Fang

Stevens

Berk

et al. 2004

J Virol

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Mouse adenovirus type 1 (MAV-1) early region 1A (E1A) encodes a virulence gene in viral infection of mice. To broaden our understanding of the functions of E1A in MAV-1 pathogenesis, an unbiased experimental approach, glutathione S-transferase (GST) pulldown, was used to screen for cellular proteins that interact with E1A protein. We identified mouse Sur2, a subunit of Mediator complex, as a protein that binds to MAV-1 E1A. The interaction between Sur2 and MAV-1 E1A was confirmed in virus-infected cells. Conserved region 3 (CR3) of MAV-1 E1A was mapped as the region required for Sur2-E1A interaction, as is the case for human adenovirus E1A. Although it has been proposed that human adenovirus E1A recruits the Mediator complex to transactivate transcription of viral early genes, Sur2 function in adenovirus replication has not been directly tested previously. Studies on the functions of Sur2 with mouse embryonic fibroblasts (MEFs) showed that there was a multiplicity-dependent growth defect of MAV-1 in Sur2 ؊/؊ MEFs compared to Sur2 ؉/؉ MEFs. Comparison of the viral DNA and viral mRNA levels in Sur2 ؉/؉ and Sur2 ؊/؊ MEFs confirmed that Sur2 was important for efficient viral replication. The viral replication defects in Sur2 ؊/؊ MEFs appeared to be due at least in part to a defect in viral early gene transcription.

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Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1

Fang

Stevens

Berk

et al. 2004

J Virol

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“…9). The DNA regions recognized by the MAd-1 probe in HAd-2 contain genes coding for structural components of the virus (Ball et al, 1988(Ball et al, , 1989Beard et al, 1990;Gingeras et al, 1982;Larsen et al, 1979;Raviprakash et at., 1989). The observation that a MAd-2 probe recognizes approximately the same sequences as the MAd-1 probe in HAd-2 DNA suggests that the two MAds have genes encoding similar hexon and structural proteins.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, physical mapping and cross-hybridization of the murine adenovirus type 1 and type 2 genomes

Jacques

Cousineau

D'Amours

et al. 1994

Journal of General Virology

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Murine adenovirus (MAd) type 1 strain FL and type 2 strain K87 genomes were cloned into plasmid pAT153 as HindIII restriction fragments. The MAd-1 and MAd-2 DNA genomes, 30"10kb and 34.71 kb in length respectively, were mapped using BgIII, ClaI, EcoRI, HindIII and SphI restriction endonuclease cleavage sites. In view of the large differences found between the MAd-1 and MAd-2 genomes in terms of the number and location of restriction sites, cross-hybridization experiments were performed. Homologous DNA sequences were located on the MAd-1 and MAd-2 physical maps. Both viruses are also genetically related to human adenovirus type 2 (HAd-2). Nucleotide sequences shared by HAd-2 and the MAds code for structural proteins, which may explain the antigenic similarities between these viruses from different origins. Our results confirm the existence of two distinct adenovirus species in the mouse.

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“…Murine adenovirus type 1 (MAV-1) has adrenal and cardiac tropism (reviewed in Ishibashi & Yasue, 1984). The MAV-1 early regions El, E3 and E4 and the late regions corresponding to IVa2 and the endoproteinase gene have been sequenced and these data suggest that the organization of the genome is similar to that found in human adenoviruses (Ball et al, 1988(Ball et al, , 1989(Ball et al, , 1991Beard et al, 1990;Cai et al, 1992;Kring & Spindler, 1990).…”

Section: Introductionmentioning

confidence: 98%

Sequence and structural analysis of murine adenovirus type 1 hexon

Weber

Cai

Murali

et al. 1994

Journal of General Virology

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The genomic region encoding the major capsid protein (hexon) of murine adenovirus type 1 (MAV-1) has been isolated and sequenced. The sequence predicts a 908 residue MAV-I hexon protein and is flanked by a portion of the upstream pVI gene and the downstream endoproteinase gene. The order of these genes and their location in the middle of the genome are the same as those found in other adenoviruses sequenced to date. Multiple sequence alignment with the other five known hexon protein sequences reveals an overall residue identity of 51% and residue conservation of 66 %. In comparison with human adenovirus type 2 (Ad2), MAV-1 hexon has major deletions between residues 141 to 170, 270 to 284 and 446 to 455. Since these regions in the Ad2 hexon are partially exposed on the outer surface of the virion, they may represent type-specific antigenic determinants. The MAV-1 hexon sequence has been modelled using the known three-dimensional structure of the Ad2 hexon. The variable regions in which the mutations, deletions and insertions occur are located in the 11 and 1~ loops of the molecule that form the protruding hexon towers on the external surface of the virion.

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Genome organization of mouse adenovirus type 1 early region 1: A novel transcription map

Cited by 34 publications

References 60 publications

Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1

Requirement of Sur2 for Efficient Replication of Mouse Adenovirus Type 1

Molecular cloning, physical mapping and cross-hybridization of the murine adenovirus type 1 and type 2 genomes

Sequence and structural analysis of murine adenovirus type 1 hexon

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