Identification of mouse adenovirus type 1 early region 1: DNA sequence and a conserved transactivating function

Abstract

The left end of the genome of mouse adenovirus type 1 (also known as strain FL) was characterized by determination of the DNA sequence, amino acid similarities with early region proteins of primate adenoviruses, and a functional assay. Several specific DNA sequence features were similar to those found in human adenoviruses, and open reading frames from this region could encode proteins similar to human adenovirus early region lA and early region 1B proteins. DNAs from this region were tested in transient-expression assays in human and mouse cells and were found to transactivate the human adenovirus type 5 early region 3 promoter fused to the chloramphenicol acetyltransferase gene. The data indicate structural and functional homologies between mouse adenovirus type 1 early region 1 and early region 1 of primate adenoviruses.

Acute respiratory infection with mouse adenovirus type 1

“…The MAV-1 virion is structurally similar to that of human adenoviruses (Wigand et al, 1977), and its genomic organization is also similar to that of human adenovirus (Meissner et al, 1997). With the exception of the early region 3 (E3) gene (Beard et al, 1990), MAV-1 gene products have sequence similarity to those of human adenoviruses (Ball et al, 1988(Ball et al, , 1989(Ball et al, , 1991Beard et al, 1990;Cauthen and Spindler, 1996;Kring et al, 1992;Kring and Spindler, 1990). Of particular interest, given the potential association between the human adenovirus early region 1A (E1A) gene and long-term sequelae of infection such as COPD (Elliott et al, 1995;Matsuse et al, 1992), MAV-1 E1A has approximately 40% similarity to human adenovirus E1A, with the strongest similarity in conserved region 2 (CR2) (Ball et al, 1988).…”

Contributions of E1A to mouse adenovirus type 1 pathogenesis following intranasal inoculation

“…The MAV-1 E1A protein sequence has approximately 40% sequence similarity compared to the human adenovirus E1A 289 aa protein in CR1, CR2 and CR3 (Ball et al, 1989). MAV-1 E1A shares functional roles with human adenovirus E1A, for example, binding to the mouse cellular proteins retinoblastoma protein (pRb), pRb-related proteins p107 and p130, and Sur2 (Fang and Spindler, 2005;Fang et al, 2004;Smith et al, 1996) and transactivating the human adenovirus type 5 E3 promoter (Ball et al, 1988). The MAV-1 model is therefore well suited to use in the study of adenovirus E1Amediated effects on pulmonary pathophysiology.…”

Lack of effect of mouse adenovirus type 1 infection on cell surface expression of major histocompatibility complex class I antigens

“…Viruses and antibodies. MAV-1 was obtained and passaged as previously described (3), and titers were determined on L929 cells. Ad5 was obtained from Arnold Berk (University of California, Los Angeles) and passaged in HeLa cells; titers were determined on 293 cells.…”