Resistant plasmid profile analysis of multidrug resistant &lt;i&gt;Escherichia coli&lt;/i&gt; isolated from urinary tract infections in Abeokuta, Nigeria

Akingbade, Olusola Abiodun; Balogun, S. A.; Ojo, D. A.; Akinduti, P. A.; Okerentugba, P. O.; Nwanze, J. C.; Okonko, I. O.

doi:10.4314/ahs.v14i4.8

Cited by 49 publications

(61 citation statements)

References 32 publications

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“…Some reports also state that this antibiotic has been facing an extensive microbial resistance across the globe. The resistance rate for this drug in the present study is greater than the result of a study in 2012 conducted at three hospitals in South West Nigeria, in which 34.5% were resistant to ceftriaxone [16]. Several studies have confirmed that Pseudomonas aeruginosa is mostly resistant against ceftriaxone [3,17,18].…”

Section: Discussioncontrasting

confidence: 54%

Antibacterial Resistance Pattern of Pseudomonas Aeruginosa Isolated From Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia

Rustini

Jamsari

Marlina

et al. 2017

Asian J Pharm Clin Res

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Objectives: Pseudomonas aeruginosa is an opportunistic pathogen that has an innate resistance to some antibiotics. This bacterium is one of the most common causes of nosocomial infections that include surgical wound infections, burns, and urinary tract infections. The bacteria have been reportedly resistant to many antibiotics and have developed multidrug resistance (MDR). The objective of the study was to determine the resistance pattern of P. aeruginosa isolated from clinical samples of patients against some major antibiotics. Methods:Isolates of P. aeruginosa were obtained from clinical sample of urine, sputum, swabs, pus, feces, and blood and cultured in cetrimide agar. P. aeruginosa ATCC 27853 was used as a positive control. The antibacterial susceptibility testing was conducted against 13 antibiotics: Ceftazidime, cefotaxime, ceftriaxone, cefoperazone, ciprofloxacin, levofloxacin, ofloxacin, gentamicin, amikacin, piperacillin, ticarcillin, meropenem, and imipenem. The examination was carried out using agar diffusion method of Kirby-Bauer and following the standards from Clinical and Laboratory Standards Institute (CLSI). Results:The results showed that bacterial resistance was established against all tested antibiotics. The highest number of resistance was shown against ceftriaxone (44.21%), whereas the most susceptibility was exhibited against amikacin (only 9.47% of resistance). MDR P. aeruginosa (MDRPA) was detected on almost all clinical samples tested, except the feces. The sample with the highest percentage of MDRPA was the pus. Conclusion:The study concludes that the most effective antibiotic against P. aeruginosa is amikacin (91.51%), whereas the most resistance is exhibited to ceftriaxone (43.16%).

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Section: Discussioncontrasting

confidence: 54%

Antibacterial Resistance Pattern of Pseudomonas Aeruginosa Isolated From Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia

Rustini

Jamsari

Marlina

et al. 2017

Asian J Pharm Clin Res

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“…The IMP type is the most clinically significant carbapenemases which encoded by blaIMP gene and harden all treatments done using the imipenem. 31 Our results also showed that about 96.03% of imipenem-resistant P. aeruginosa strains harbored the blaIMP-1 gene which was considerable. Peymani et al (2015) 32 revealed that 107 (35.66%) P. aeruginosa isolates were non-susceptible to imipenem and/or meropenem and among them, 56 (52.3%) isolates were MBL producer.…”

Section: Discussionsupporting

confidence: 59%

Prevalence of antibiotic resistance and blaIMP-1 gene among Pseudomonas aeruginosa strains isolated from burn and urinary tract infections in Isfahan, central Iran

Hashemi

Mojiri

Kachouyi

et al. 2017

Microbiol Res (Pavia)

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Pseudomonas aeruginosa is one of the most important opportunistic pathogens responsible for various types of hospital infections. High prevalence of antibiotic resistance in P. aeruginosa strains of human clinical samples cause more severe diseases for a longer period of time. The current research was done in order to study the distribution of blaIMP-1 gene among the imipenem-resistant P. aeruginosa strains isolated from burn and urinary tract infections of hospitalized patients. Two-hundred and forty-three P. aeruginosa isolates recovered from the cases of burn and urinary tract infections of inpatients and outpatients were analysis for antibiotic resistance pattern using the disk diffusion method. Then, imipenem-resistant isolates were further analyzed for distribution of blaIMP-1 gene using the PCR. Of 243 P. aeruginosa isolates, 146 strains (60.08%) were taken from outpatients and 97 strains (39.91%) were taken from inpatients. P. aeruginosa isolates harbored the highest levels of resistance against streptomycin (100%), nalidixic acid (100%), aztreonam (100%), cotrimoxazole (95.47%), ciprofloxacin (88.47%), cefotaxime (84.36%) and gentamycin (83.95%). Inpatients had a relatively higher levels of antibiotic resistance. One-hundred and twenty-one out of 126 (96.03%) imipenem-resistant P. aeruginosa isolates harbored the blaIMP-1 gene. Inpatients also had a relatively higher prevalence of blaIMP-1 gene. High prevalence of blaIMP-1 gene and also imipenemresistant P. aeruginosa are important public health issue. Clinical laboratories should consider the detection of the blaIMP-1 gene among the P. aeruginosa isolates of clinical samples.

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“…In this study, the percentage of papC gene was 10% which is lower than the result obtained by (Rocha et al, 2008) (Olayinka et al, 2009) who reported that P. aeruginosa showed resistance to ciprofloxacin and chloramphenicol at a percentage of 90.2% and 97.8%, respectively but higher than that reported by (Akingbade et al, 2012) (Moniri and dastehgoli, 2007) who reported that E. coli showed resistance to ciprofloxacin at a percentage of 69.7% but higher than that reported by (Miles et al, 2006) who found that E. coli showed resistance to ciprofloxacin, norfloxacin and and ofloxacin at a percentage of 11.8%, 20.6% and 14.7%, respectively and this higher resistance may be due to frequent using of the same antibiotic in treatment which lead to resistance to this antibiotic but lower than that reported by (Jahantigh and Reza, 2015) who said that E. coli isolates were resistant to ciprofloxacin and norfloxacin at a percentage of 91% and 88%, respectively.…”

Section: Suspectedcontrasting

confidence: 54%

Molecular Characterization of Some Bacteria Isolated from Broiler Chickens Showing Respiratory Manifestations

Elghazaly¹,

Sedeek²,

Khalil³

2017

AJVS

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Key words:E. coli, P. aeruginosa, virulence genes, antibiogram, plasmid profile Fermented wheat germ extract (FWGE) is a multisubstance composition contains 2-methoxy benzoquinone and 2, 6-dimethoxy benzoquinone which are likely to exert some of its biological effects as well as it is a concentrated source of vitamins, minerals, and protein.An experimental trial of FWGE supplementation to broiler feed from one day old with a rate of 0.5, 1.5 and 3 g/kg feed was tried. Results revealed that all doses of FWGE increased body weight significantly (p≤0.05), especially with the dose of 3 g/kg feed. Also, FCR values decreased significantly (p≤0.05) in the FWGE treated groups. Regarding biochemical analysis at 35 days old, the most significant results obtained with the doses of 1.5 and 3 g FWGE/kg feed especially in SGOT, SGPT, creatinine, uric acid, total protein, glucose, and triglycerides levels. Physiologically, FWGE only increased hemoglobin concentration significantly (p≤0.05) without alteration of red blood and white blood cells counts. There was also a significant increase (p≤0.05) in the intestinal weight in relation to carcass weight% and a significant decrease (p≤0.05) in the liver and total body fat weights in relation to carcass weight%. At 45 days (10 days after vNDV challenge), the mortality rates were 60% in the non-treated non-vaccinated challenged chicken group 5 and 4% in the nontreated vaccinated challenged chicken group 4 without appearance of any mortality in the 3 FWGE treated groups. Also, addition of FWGE had a positive effect on HI titers for NDV in the collected serum samples at 45 days old. Finally, it was concluded that FWGE improved the general health condition of broilers regarding biochemical and physiological parameters and immune response to NDV vaccination.

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Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Cited by 49 publications

References 32 publications

Antibacterial Resistance Pattern of Pseudomonas Aeruginosa Isolated From Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia

Antibacterial Resistance Pattern of Pseudomonas Aeruginosa Isolated From Clinical Samples at a General Hospital in Padang, West Sumatra, Indonesia

Prevalence of antibiotic resistance and blaIMP-1 gene among Pseudomonas aeruginosa strains isolated from burn and urinary tract infections in Isfahan, central Iran

Molecular Characterization of Some Bacteria Isolated from Broiler Chickens Showing Respiratory Manifestations

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