Prevalence of antibiotic resistance and blaIMP-1 gene among Pseudomonas aeruginosa strains isolated from burn and urinary tract infections in Isfahan, central Iran

Abstract

Pseudomonas aeruginosa is one of the most important opportunistic pathogens responsible for various types of hospital infections. High prevalence of antibiotic resistance in P. aeruginosa strains of human clinical samples cause more severe diseases for a longer period of time. The current research was done in order to study the distribution of blaIMP-1 gene among the imipenem-resistant P. aeruginosa strains isolated from burn and urinary tract infections of hospitalized patients. Two-hundred and forty-three P. aeruginosa isolates recovered from the cases of burn and urinary tract infections of inpatients and outpatients were analysis for antibiotic resistance pattern using the disk diffusion method. Then, imipenem-resistant isolates were further analyzed for distribution of blaIMP-1 gene using the PCR. Of 243 P. aeruginosa isolates, 146 strains (60.08%) were taken from outpatients and 97 strains (39.91%) were taken from inpatients. P. aeruginosa isolates harbored the highest levels of resistance against streptomycin (100%), nalidixic acid (100%), aztreonam (100%), cotrimoxazole (95.47%), ciprofloxacin (88.47%), cefotaxime (84.36%) and gentamycin (83.95%). Inpatients had a relatively higher levels of antibiotic resistance. One-hundred and twenty-one out of 126 (96.03%) imipenem-resistant P. aeruginosa isolates harbored the blaIMP-1 gene. Inpatients also had a relatively higher prevalence of blaIMP-1 gene. High prevalence of blaIMP-1 gene and also imipenemresistant P. aeruginosa are important public health issue. Clinical laboratories should consider the detection of the blaIMP-1 gene among the P. aeruginosa isolates of clinical samples.

High Incidence of MDR and XDR Pseudomonas aeruginosa Isolates Harboring blaGES, blaVEB, and blaPER Extended-Spectrum Beta-Lactamase Genes in Iran

“…In another study, the statistical analysis results showed that the incidence rate of MDR/XDR in uropathogenic bacteria (including P. aeruginos), obtained from inpatients was significantly higher than in those obtained from out-patients [30] . In a study by Hashemi et al (2017), resistance to most antibiotics was higher in P. aeruginosa isolates isolated from hospitalized patients compared to those isolated from out-patients [31] . In addition, Farajzadeh Sheikh et al (2020) found that the prev-alence of MDR P. aeruginosa isolates was higher among patients admitted to the burn ward compared to other wards [32] .…”

DNA Analysis of qacE Gene in Pseudomonas Aeruginosa Isolated from Iraqi Patient

“…Bacteria also have a greenish-yellow pyoverdin pigment, and these pigments are dissolved in water [12,13] in addition to having a layer of alginate and a biofilm, which increases its resistance to antibiotics [14] Resistance of P. aeruginosa to antibiotics and disinfectants is a major problem all over the world due to the random and unrestricted use of antibiotics [15]. This resistance is also likely to occur due to several reasons, including the production of beta-lactamase enzymes and the lack of permeability of their cell wall, as well as the possibility of it containing and acquiring resistance genes from another bacterial genus by plasmids and transposons via bacterial conjugation and the occurrence of genetic mutations in the genes leading to the development of resistance to these antiseptics and causing failure in treating infections caused by P.aeruginosa bacteria [16,17].…”