Researches ConcerningIn VitroConservation of the Rare Plant SpeciesDianthus NardiformisJanka

Holobiuc, Irina; Blindu, Rodica; Cristea, Vasile

doi:10.1080/13102818.2009.10818405

Cited by 14 publications

(13 citation statements)

References 6 publications

(4 reference statements)

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“…The worst results in our experiment were achieved with sodium dichloroisocyanurate (DICA), with the biggest contamination of explants among all sterilizing agents used. Holobiuc et al (2009) reported that DICA also was the least efficient sterilizing agent for Dianthus nardiformis, comparing it with HgCl 2 , NaOCl and H 2 O 2 On the contrary, Osterc et al (2004) reported that reduction in contamination by using 16.6 g/L DICA for 15-min duration was very successful for vigorous cherry plants. Parkinson et al (1996) reported that sodium dichloroisocyanurate was more effective than commercially available bleach for disinfection.…”

Section: Resultsmentioning

confidence: 99%

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

et al. 2013

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Surface sterilization is the most important step in preparation of explants for micropropagation, because controlling fungal and bacterial contamination of woody plant from field sources is very difficult. Six sterilizing agents: sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO) 2 ], sodium dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl 2), silver nitrate (AgNO 3) and hydrogen peroxide (H 2 O 2) were tested for sterilization of 'Oblačinska' sour cherry buds, by varying their concentration and time of exposure. The aim of this study was to establish best surface sterilization for in vitro propagation of 'Oblačinska' sour cherry. Aseptic cultures of 'Oblačinska' sour cherry were established from axillary buds which were placed in nutrient medium, supplemented with plants hormones 6-benzylaminopurine (BA), 1naphthaleneacetic acid (NAA) and gibberellic acid (GA 3). The results indicated that among these sterilizing agents silver nitrate (AgNO 3) at concentration of 1% for 20 minutes was the best for controlling the infection, whereas sterilization with sodium dichloroisocyanurate (DICA) at concentration of 1% for 10 minutes was not satisfactory.

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Section: Resultsmentioning

confidence: 99%

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

et al. 2013

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“…ssp. simonkaianus (Péterfi) Tutin (Miclăuş et al, 2003), D. callizonus Schott & Kotschy (Holobiuc and Blindu, 2006), D. nardiformis Janka (Holobiuc et al, 2009(Holobiuc et al, , 2010a, D. pratensis M. Bieb. subsp.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of the Endangered and Decorative Specie Dianthus serotinus Waldst. et Kit.

Marković

Grbić

Djukić

2013

Not Bot Hort Agrobot Cluj

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During past decades, great attention has been paid to propagation of endangered plant taxa in order to preserve biodiversity. The aim of this study was to optimize a protocol for in vitro propagation of the critically endangered and decorative species Dianthus serotinus Waldst. et Kit. The effects of different concentration of MS salt (Murashige and Skoog) of the culture, medium pH and different carbohydrates (sucrose, glucose, and fructose) on shoot multiplication were examined. The best results were obtained on half-strength MS (Murashige and Skoog) medium, whose pH was 5.8, with sucrose supplied at a concentration of 3%, when shoots with 1-2 nodes or shoot tips (with terminal buds only) were used as explants. The shoots were rooted (76.7%) on half-strength MS medium containing 0.5 mg∙L-1 NAA (1-naphthaleneacetic acid). The obtained plantlets were successfully acclimatized (89%) in a 4:1 mixture of peat and sand and they flowered the following year. Presented protocol enables successful in vitro propagation of D. serotinus.

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“…simonkaianus (Miclăuş et al, 2003), D. glacialis subsp. gelidus (Cristea et al, 2006), D. nardiformis (Holobiuc et al, 2009), D. henteri (Cristea et al, 2010), and D. giganteus subsp. banaticus (Cristea et al, 2006;Jarda et al, 2011).…”

Section: In Vitro Multiplicationmentioning

confidence: 99%

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Jarda¹,

Butiuc-Keul²,

Höhn³

et al. 2014

Turk J Biol

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Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

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Researches ConcerningIn VitroConservation of the Rare Plant SpeciesDianthus NardiformisJanka

Cited by 14 publications

References 6 publications

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

Micropropagation of the Endangered and Decorative Specie <i>Dianthus serotinus</i> Waldst. et Kit.

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

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