In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

Abstract

Surface sterilization is the most important step in preparation of explants for micropropagation, because controlling fungal and bacterial contamination of woody plant from field sources is very difficult. Six sterilizing agents: sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO) 2 ], sodium dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl 2), silver nitrate (AgNO 3) and hydrogen peroxide (H 2 O 2) were tested for sterilization of 'Oblačinska' sour cherry buds, by varying their concentration and time of exposure. The aim of this study was to establish best surface sterilization for in vitro propagation of 'Oblačinska' sour cherry. Aseptic cultures of 'Oblačinska' sour cherry were established from axillary buds which were placed in nutrient medium, supplemented with plants hormones 6-benzylaminopurine (BA), 1naphthaleneacetic acid (NAA) and gibberellic acid (GA 3). The results indicated that among these sterilizing agents silver nitrate (AgNO 3) at concentration of 1% for 20 minutes was the best for controlling the infection, whereas sterilization with sodium dichloroisocyanurate (DICA) at concentration of 1% for 10 minutes was not satisfactory.

Rooting of Stem Cuttings with Different Indole 3 Butyric Acid (IBA) Treatments and Development of Micropropagation Protocol for <i>Piper betle</i> L. Node Culture

“…The step can be undertaken by using different agents like calcium hypochlorite, sodium hypochlorite, ethanol, mercuric chloride, hydrogen peroxide or silver nitrate [36]. Generally, the type of the explants showed the main component where internodes were more preferred compared to the shoots apex.…”

Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnumL

“…They used two sterilizing agents, NaOCl(1%) and HgCl 2 (0.1%) with three time duration 2, 5 and 8 minutes, and found that NaOCl for 8 minute was better for controlling the infection and had not any adverse effect on explants even in long duration.On the other hand, [19]reported that both sterilizing agents, HgCl 2 and NaOCl, were effective in making clean explants of Kinnow tree. Nevertheless, [20]showed treatment with sodium hypochlorite (NaOCl), calcium hypochlorite Ca(ClO) 2 and (HgCl 2 ) showed satisfactory results and [21] also showed that the best sterilizing agents were 3% HgCl 2 , 3% NaOCl which reduced bacterial contaminations up to 95%.…”

Quality and Sensory Study of Fresh Sour Cherry Juices Upon Cultivar, Growing Area and Weather Conditions

“…Many sour cherry cultivars are known as continental cultivars (Ganopoulos et al., ; Grafe & Schuster, ; Homoki et al., ; Khoo et al., ; Papp et al., ; Pissard et al., ; Siddiq et al., ; Viljevac et al., ; Wojdyło et al., ), mainly grown in north and middle European countries (Clausen et al., ; Khoo et al., ; Wojdyło et al., ) as well as in Balkan region (Mihaljević et al., ). On the other hand, there are cultivars that achieve its best quality in Mediterranean climate conditions, like autochthonous cultivar Maraska grown in Dalmatia region (Levaj et al., ; Pedisić, Dragović‐Uzelac, Levaj, & Škevin, ; Repajić et al., ; Viljevac et al., ; Viljevac Vuletić et al., ).…”