Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnumL

doi:10.20431/2349-0365.0401002

Cited by 20 publications

(15 citation statements)

References 8 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In support of our observation, mercuric chloride (HgCl 2 ) was also reported to be beneficial in reducing contamination in in vitro culture of other Kaempferia species (Shirin et al 2000;Parida et al 2010). Mahmoud and Al-Ani (2016) found that HgCl 2 mainly has bactericidal action and is a more effective sterilant with better decontamination percentages compared to sodium hypochlorite. In this study, treatment with HgCl 2 was necessary and very effective for controlling contamination in K. parviflora.…”

Section: Discussionsupporting

confidence: 70%

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

Labrooy

Abdullah

Stanslas

2020

TLSR

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 70%

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

Labrooy

Abdullah

Stanslas

2020

TLSR

View full text Add to dashboard Cite

show abstract

“…Furthermore, tissue culture has an enabling technology role in plant molecular breeding and biotechnology (Da Silva et al 2019). However, it should be stated that the ability to rapidly propagate plants in vitro has its own challenges such as different genotypic characteristics, explant survival, phytohormones, culture medium composition, and culture conditions (Dobránszki and Da Silva 2010, Magyar-Tábori et al 2010, Da Silva et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

Kang¹,

Zhou²,

Jiang³

et al. 2021

Biologia plant.

View full text Add to dashboard Cite

Rapid propagation of plants by tissue culture is of great significance for large-scale production, molecular genetics research, and breeding. Currently, a rapid and high-efficient tissue culture protocol for Euonymus bungeanus is needed. To develop a propagation system for this species, we established a new regeneration system from leaves. Callus formation was induced on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm -3 6-benzylaminopurine (6-BA) and 0.5 mg dm -3 α-naphthalene acetic acid (NAA) and the induction rate almost reached 100 % under red radiation within 21 d. The medium for proliferation of adventitious buds comprised of MS medium with 1.0 mg dm -3 6-BA and 0.5 mg dm -3 NAA, and the induction rate within 20 d nearly reached 100 %. When, the adventitious buds were transferred to the rooting medium containing ½MS, 2.0 mg dm -3 indole-3-butyric acid (IBA), and 0.05 mg dm -3 NAA, adventitious root formation was achieved within 20 d. Collectively, the rapid and high-efficient regeneration system from E. bungeanus leaves was established, providing useful references for effective mass propagation and it could serve as an enabling technology for future genetic engineering.

show abstract

“…In this study, both explants were treated with 70% ethanol for sterilization purpose. Generally, 70% ethanol is used as pre-treatment with other sterilizing agent and aid well in sterilization, but it is also known for its phototoxic effects and dehydration ability towards plant tissue (Mahmoud and Al-Ani, 2016). The effectiveness of ethanol is dependent on the exposure time, where overexposure can reduce the rate of success (Tewelde et al, 2020).…”

Section: Explant Surface Sterilizationmentioning

confidence: 99%

OPTIMISATION OF CULTURE CONDITION FOR SACHA INCHI (Plukenetia Volubilis) CALLUS INDUCTION

Rosle¹,

Jalil²,

Wahab³

et al. 2021

Trop.cal.Agr

View full text Add to dashboard Cite

Plukenetia volubilis or commonly known as sacha inchi is reported to produce wide range of health-promoting bioactive metabolites. These metabolites functions as supplements in eradicating various types of diseases. Sacha inchi has large edible seeds that are rich in phenolic content, minerals and essential fatty acid, such as omega 3 (ω-3), omega 6 (ω-6), omega 7 (ω-7), and omega 9 (ω-9). In vitro cultures could serve as alternative in producing many essential sacha inchi bioactive compounds. As an initial step towards initiating in vitro culture, the effect of 2,4-D and TDZ on inducing callus cultures were investigated. Two different explants, sacha inchi leaf and male flower were used in this study. Surface sterilization of sacha inchi was first optimized to overcome culture contamination. The most effective surface sterilization is by using 70% ethanol (30 seconds) and 0.5% sodium hypochlorite (8 minutes), which resulted in 82.5% and 95% survival rate for leaf and flower explants respectively. Next, for calli induction the explants were cultured on MS medium supplemented with different concentrations of 2,4-D and TDZ, either alone or in combination and grown at 24 hours dark photoperiods. The morphology and size of callus were observed. The results obtained from the experiment varied depending on the treatments, producing either friable or compact calli of creamy white, pure white or brownish colour. For both, leaf and male flower explants, MS medium supplemented with 3% (w/v) sucrose in combination with 1.0 mg/L 2,4-D and 0.005 mg/L TDZ recorded the best response in term of callus size, forming friable creamy white callus.

show abstract

Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnumL

Cited by 20 publications

References 8 publications

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

OPTIMISATION OF CULTURE CONDITION FOR SACHA INCHI (Plukenetia Volubilis) CALLUS INDUCTION

Contact Info

Product

Resources

About