Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Abstract

Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

In vitro multiplication of Dianthus carthusianorum calamine ecotype with the aim to revegetate and stabilize polluted wastes

“…The development of effective micropropagation protocol is particularly valuable in the case of this unique flora whose representatives are described in varied ecological niches. A good example of this kind of scientific activity is multiplication of metal-tolerant species which grow on terrains with high metallic background (Doran 2009;Cristea et al 2013;Jarda et al 2014;Slazak et al 2015), as the efficient regeneration protocol of Thlaspi caerulescensperhaps the most famous hyperaccumulator of zinc and cadmium or Pteris vittata-fern conducting phytovolatilization of arsenic (Zheng et al 2008;Shukla and Khare 2014). Nevertheless, the investigations with the use of in vitro techniques for metallophyte propagation are still limited in comparison with well elaborated plant tissue culture protocols of numerous cultivated species (Bidwell et al 2001;Jack et al 2005;Hanus-Fajerska et al 2009, 2012Zhao et al 2009;Wiszniewska et al 2015).…”

“…Similarly, MS was used in experimental work on D. spiculifolius (Cristea et al 2013) or D. giganteus ssp. banaticus (Jarda et al 2014) carried out in order to protect biodiversity. Another example could be D. caryophyllus that is one of the world's most popular ornamental plant (Kharrazi et al 2011;Esmaiel et al 2013).…”

Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers

“…banaticus, D. spiculifolius, and Pyrus pyraster, etc. (Butiuc-keul, Farkas, & Cristea, 2016;Condello, Palombi, & Tonelli, 2008;Cristea, Crăciunaș, Marcu, Palada, & Butiuc-Keul, 2014;Jarda, Butiuc-Keul, Höhn, Pedryc, & Cristea, 2014;Tiwari et al, 2013). The SSR primers developed in this study could complement and enrich the existing SSR markers for banana species.…”

Td-DAMD-PCR assays for fingerprinting of commercial carnations

“…The Td-DAMD-PCR markers were obtained at 55 °C annealing temperature, which is a temperature higher than those used in RAPD and ISSR systems. Although RAPD and ISSR systems have been effectively used in Dianthus (Fu et al, 2008;Behroozian et al, 2013;Wojcik et al, 2013;Jarda et al, 2014), the development of a new DNA marker system is always useful for a crop. For instance, new DNA markers (like Td-DAMD-PCR in carnations) can be useful to support the variety registration system under the seeds and seedlings law based on UPOV (The International Union for the Protection of New Plant Varieties, Switzerland) and for fingerprinting studies.…”