Td-DAMD-PCR assays for fingerprinting of commercial carnations

İnce, Ayşe Gül; Karaca, Mehmet

doi:10.3906/biy-1407-66

Cited by 8 publications

(2 citation statements)

References 34 publications

(43 reference statements)

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“…), carnations are among the most popular ornamental flowers in the world. More than 30,000 varieties of carnations have been cultivated worldwide [1], and are fancied by growers and consumers for their large flowers, numerous varieties with diverse colors, and long vase life. Flower color in carnations has long been an important target of artificial selection by plant breeders who are well aware of the economic benefits that new color varieties can yield [2].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the MYB and bHLH Families in Carnations and Expression Analysis at Different Floral Development Stages

Leng

Zhang

Liu

et al. 2023

IJMS

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Carnations are one of the most popular ornamental flowers in the world with varied flower colors that have long attracted breeders and consumers alike. The differences in carnation flower color are mainly the result of the accumulation of flavonoid compounds in the petals. Anthocyanins are a type of flavonoid compound that produce richer colors. The expression of anthocyanin biosynthetic genes is mainly regulated by MYB and bHLH transcription factors. However, these TFs have not been comprehensively reported in popular carnation cultivars. Herein, 106 MYB and 125 bHLH genes were identified in the carnation genome. Gene structure and protein motif analyses show that members of the same subgroup have similar exon/intron and motif organization. Phylogenetic analysis combining the MYB and bHLH TFs from Arabidopsis thaliana separates the carnation DcaMYBs and DcabHLHs into 20 subgroups each. Gene expression (RNAseq) and phylogenetic analysis shows that DcaMYB13 in subgroup S4 and DcabHLH125 in subgroup IIIf have similar expression patterns to those of DFR, ANS, and GT/AT, which regulate anthocyanin accumulation, in the coloring of carnations, and in red-flowered and white-flowered carnations, DcaMYB13 and DcabHLH125 are likely the key genes responsible for the formation of red petals in carnations. These results lay a foundation for the study of MYB and bHLH TFs in carnations and provide valuable information for the functional verification of these genes in studies of tissue-specific regulation of anthocyanin biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the MYB and bHLH Families in Carnations and Expression Analysis at Different Floral Development Stages

Leng

Zhang

Liu

et al. 2023

IJMS

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“…for Salvia species (Ince and Karaca 2012); Allium sp. (Deniz et al 2013); carnation cultivars (Ince and Karaca 2015), commercial cotton (Gocer and Karaca 2016) and S. tomentosa (Saleh 2019b).…”

Section: Introductionmentioning

confidence: 99%

TU-DAMD employment for molecular characterization of Salvia judaica and Salvia palaestina species

Saleh

2021

Acta Biol Szeged

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Genetic diversity in perennial Salvia judaica Boiss (Judean sage) and Salvia palaestina Benth (Palestinian sage) species using touch-up directed amplification of minisatellite region DNA (TU-DAMD) has been performed in two separated sets; in the first set (set A) the initial annealing temperature was increased from 50 °C to 55 °C, whereas, in the second one (set B), it increased from 55 °C to 60 °C by 0.5 °C/cycle during the first 10 PCR amplification cycles. Fifteen DAMD primers have been tested for each set. Set (A) produced 89.39% polymorphism level (P%) with polymorphic information content (PIC) average of 0.33 and marker index (MI) average of 3.96. Whereas, in set (B) these values were recorded to be 94.02%, 0.34 and 3.98 for P%, PIC and MI, respectively. Data showed that the two mentioned sets successfully highlighted high polymorphism level between the two studied Salvia sp. This work studies genetic diversity of S. judaica and S. palaestina species using TU-DAMD test as a novel molecular marker.

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Construction of Linkage Maps and Development of Useful DNA Markers for Carnation Breeding

Yagi¹

2020

Compendium of Plant Genomes

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Td-DAMD-PCR assays for fingerprinting of commercial carnations

Cited by 8 publications

References 34 publications

Genome-Wide Identification of the MYB and bHLH Families in Carnations and Expression Analysis at Different Floral Development Stages

Genome-Wide Identification of the MYB and bHLH Families in Carnations and Expression Analysis at Different Floral Development Stages

TU-DAMD employment for molecular characterization of Salvia judaica and Salvia palaestina species

Construction of Linkage Maps and Development of Useful DNA Markers for Carnation Breeding

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