Research in quantitative microscopic X-ray fluorescence analysis

Lankosz, M.; Szczerbowska-Boruchowska, Magdalena; Chwiej, Joanna; Ostachowicz, J.; Simionovici, Alexandre; Bohic, Sylvain

doi:10.1016/j.sab.2004.07.016

Cited by 18 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The study on quantitative µ-XRF analysis of biological materials and glass samples conducted by Lankosz research group was summarized in Ref. [66]. Glass samples of various thicknesses were analyzed by FP approach using µ-XRF spectrometer utilizing capillary optics with effective beam diameter of ca.…”

Section: Fundamental Parameters Methods In µ-Xrf Spectrometrymentioning

confidence: 99%

Quantitative X-ray fluorescence analysis of samples of less than ‘infinite thickness’: Difficulties and possibilities

Sitko

2009

Spectrochimica Acta Part B: Atomic Spectroscopy

View full text Add to dashboard Cite

Section: Fundamental Parameters Methods In µ-Xrf Spectrometrymentioning

confidence: 99%

Quantitative X-ray fluorescence analysis of samples of less than ‘infinite thickness’: Difficulties and possibilities

Sitko

2009

Spectrochimica Acta Part B: Atomic Spectroscopy

View full text Add to dashboard Cite

“…Since less energy is deposited in the sample as a result of X-ray excitation, compared to electron or charged-particle excitation sources, thermal damage to the samples and associated problems for loss of trace elements by volatilization, and also a redistribution of elements through the sample can be avoided. 6,7 The concentration was determined according to:…”

Section: X-ray Microfluorescencementioning

confidence: 99%

Topographic Trace-Elemental Analysis in the Brain of Wistar Rats by X-ray Microfluorescence with Synchrotron Radiation

et al. 2008

View full text Add to dashboard Cite

Knowledge about the spatial distribution and the local concentration of trace elements in tissues is of great importance, since trace elements are involved in many biological functions of living organisms. However, there are few methods available to measure the spatial (two (three)-dimensional) elemental distribution in animal brain. X-ray microfluorescence with synchrotron radiation is a multielemental mapping technique, which was used in this work to determine the topographic of iron, zinc and copper in coronal sections of female Wistar rats of different ages. Young (14 days old) and middle-aged (20 months old) rats (n = 8) were analyzed. The measurements were carried out at the XRF beam line at the Synchrotron Light National Laboratory (Campinas, Brazil). Two-dimensional scanning was performed in order to study the tendency of elemental concentration variation. The acquisition time for each pixel was 10 s/step and the step size was 300 mm/step in both directions. It was observed that the iron distribution was more conspicuous in the cortical area, thalamus and bellow the thalamus. On the other hand, the zinc distribution was more pronounced in the hippocampus. The iron, copper and zinc levels increased with advancing age. Therefore, this study reinforces the idea that these elements are involved in the chemical mechanisms of the brain that induce some neurological diseases, since they are also present in high levels in specific areas of the brain, such as the hippocampus and the substantia nigra of patients with these disorders.

show abstract

“…X‐ray microfluorescence (μXRF) technique is a method, based on localized excitation of a microscopically small (on the order of μm) area of the sample that allows the investigation of changes in the elemental composition. This is particularly important in view of the fact that normal and pathological biochemical processes occur at the micro and submicron range . Synchrotron radiation (SR) generates quite strong X‐rays (and other electromagnetic waves, such as ultra violet, visible, and infrared light).…”

Section: Introductionmentioning

confidence: 99%

“…This is particularly important in view of the fact that normal and pathological biochemical processes occur at the micro and submicron range. [6][7][8] Synchrotron radiation (SR) generates quite strong X-rays (and other electromagnetic waves, such as ultra violet, visible, and infrared light). Its intensity is higher than laboratory X-ray sources by several orders of magnitude.…”

Section: Introductionmentioning

confidence: 99%

Zinc distribution in human prostate carcinoma cell line using synchrotron X‐ray microfluorescence

et al. 2017

View full text Add to dashboard Cite

a Cancer is a worldwide public health problem, and its incidence in the world grew by 20% in the last decade. Zinc, an essential trace element, is involved in many cellular processes. Concerning prostate cancer, Zn could inhibit the growth of tumor cells, by inducing cell cycle arrest or apoptosis. X-ray microfluorescence (μXRF) is an elemental analysis technique that allows mapping biologically important elements at a submillimeter scale, with high sensitivity and negligible damage to the sample. In this study, we investigated cell viability through colorimetric cytotoxicity assay (MTT) and the behavior of human prostate cell lines obtained from normal (RWPE-1) and tumorigenic (DU145) epithelium, in three-dimensional spheroid cultures after supplementation with ZnCl 2 for 24 and 48 hr using synchrotron μXRF. The measurements were performed at the Synchrotron Light National Laboratory (Campinas, Brazil). The results of μXRF showed that Zn intensity decreased in the DU145 tumor cells independent of supplementation or treatment time, and in normal cells, the intensity increased with supplementation and treatment time. The MTT assay results showed no significant differences, which indicates that the cell viability did not change. Our results indicate that μXRF can be used as an important tool to understand the mechanism of the loss of ability to capture zinc by prostate cancer cells.

show abstract

Research in quantitative microscopic X-ray fluorescence analysis

Cited by 18 publications

References 3 publications

Quantitative X-ray fluorescence analysis of samples of less than ‘infinite thickness’: Difficulties and possibilities

Quantitative X-ray fluorescence analysis of samples of less than ‘infinite thickness’: Difficulties and possibilities

Topographic Trace-Elemental Analysis in the Brain of Wistar Rats by X-ray Microfluorescence with Synchrotron Radiation

Zinc distribution in human prostate carcinoma cell line using synchrotron X‐ray microfluorescence

Contact Info

Product

Resources

About