Research Article Genetic diversity of the mangaba GeneBank using microsatellites

Abstract

The mangaba, Hancornia speciosa, (Apocynaceae) is a fruit tree native to Brazil with predominantly extractivist production. The fruit can be consumed in natura; however, it is widely consumed as frozen pulp and ice cream produced by agroindustry companies. We evaluated the genetic diversity of 213 individuals that make up the mangaba GeneBank of Embrapa Tabuleiros Costeiros, using nine microsatellite markers (SSR). A total of 147 alleles were identified, with a mean of 16 alleles per locus; 100% polymorphism was observed among accessions. Reliability of the result was verified based on stress (0.042) and correlation (0.988) values. The alleles presented a high frequency of heterozygosity (He > Ho). The F st (0.22) and f (0.07) values indicated moderate population structure, with great diversity within accessions. Bayesian analysis indicated the most adequate grouping with k = 2. The Unweighted Pair Group Method analysis showed three distinct groups according to similarity. The BI accession had the best genetic structure. The PM5/GX2, CN1/CN9, G18/PA1, JA14/JA15, and OI8/OI9 pairs of individuals are the closest genetically. We conclude ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (1): gmr18108 A.V.C. Silva et al. 2 that the Mangaba GeneBank has high diversity; this knowledge is relevant to develop strategies for the management of these genetic resources.

Identification and preliminary characterization of early fruiting mangabeira (Hancornia speciosa – Apocynaceae)

“…Some years later, Santos et al (2017) analysed the accession genetic diversity in the fruiting phase, using ISSR markers. Recently, Silva et al (2019) used microsatellite primers tested by Amorim et al (2015) and confirmed the genetic diversity of this germplasm.…”

“…Recently, research on its functional and nutritional properties has occurred (SANTOS et al, 2017). Due to the expansion of the land-development and deforestation, the areas where the species naturally occurs have suffered rapid reduction in the last decade, especially in the northeastern coastal region (SILVA et al, 2019).…”

“…Recentemente, tem-se desenvolvido algumas pesquisas sobre suas propriedades funcionais e nutracêuticas (SANTOS et al, 2017). Devido à expansão da atividade imobiliária e ao desmatamento, as áreas de ocorrência natural da espécie sofreram redução acelerada na última década, principalmente na região costeira nordestina (SILVA et al, 2019).…”

“…Anos depois, Santos et al (2017) avaliaram a diversidade genética dos acessos em fase de frutificação, utilizando marcadores ISSR (Inter repetições de sequências simples). Recentemente, Silva et al (2019) utilizaram os primers microssatélites testados por Amorim et al (2015) e confirmaram a diversidade genética desse germoplasma.…”

Pharmaceutical, food potential, and molecular data of Hancornia speciosa Gomes: a systematic review

“…Studies of genetic diversity that were estimated by the molecular markers were also among the most developed themes. Numerous studies that used molecular markers, such as RAPD (Silva et al 2013 ), ISSR (Da Costa et al 2015 ), and SSR (Silva et al 2019 ), to assess the natural populations (Silva et al 2017b ) and variability in germplasm banks (Silva et al 2019 ). The significant themes focused on the use as food and as a medicinal plant, and studies of natural populations in different regions, where the plant is known and is used mainly by extractive communities to treat diseases and as food has been carried out.…”