Repair of bone defects by applying biomatrices with and without autologous osteoblasts

Henkel, Kai-Olaf; Gerber, Thomas; P, Dörfling; Gundlach, Karsten K.H.; Bienengräber, V.

doi:10.1016/j.jcms.2004.08.005

Cited by 47 publications

(33 citation statements)

References 13 publications

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“…Recently, a granular material consisting of nanocrystalline HA embedded in a silica gel matrix [8] with an extremely large internal surface and a material porosity of about 60% [9] has been developed and approved. Animal experiments using this nanocrystalline HA (ncHA) in the mini pig critical size defect model showed a significantly higher rate of bone formation compared to other HA and TCP materials or gelatine sponges and a nearly complete resorption 8 months after implantation [10,11] giving an initial insight into the cellular processes of osteoconduction and early remodelling in vivo [11][12][13][14]. Furthermore, the recruitment and occurrence of Runx-2-positive osteoblast precursor cells and the upregulation of BMP-2 in sites grafted by the ncHA in humans suggests that this material has osteoinductive properties [13].…”

Section: Introductionmentioning

confidence: 99%

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Götz

Lenz²,

Reichert³

et al. 2011

Folia Histochem Cytobiol.

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Abstract:To test the probable osteoinductive properties of NanoBone®, a new highly non-sintered porous nano-crystalline hydroxylapatite bone substitute embedded into a silica gel matrix, granules were implanted subcutaneously and intramuscularly into the back region of 18 mini pigs. After periods of 5 and 10 weeks as well as 4 and 8 months, implantation sites were investigated using histological and histomorphometric procedures. Signs of early osteogenesis could already be detected after 5 weeks. The later periods were characterized by increasing membranous osteogenesis in and around the granules leading to the formation of bone-like structures showing periosteal and tendon-like structures with bone marrow and focal chondrogenesis. Bone formation was better in the subcutaneous than in the intramuscular implantation sites. This ectopic osteogenesis is discussed with regard to the nanoporosity and microporosity of the material, physico-chemical interactions at its surface, the differentiation of osteoblasts, the role of angiogenesis and the probable involvement of growth factors. The results of this preliminary study indicate that this biomaterial has osteoinductive potential and induces the formation of bone structures, mainly in subcutaneous adipose tissue in the pig.

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Section: Introductionmentioning

confidence: 99%

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Götz

Lenz²,

Reichert³

et al. 2011

Folia Histochem Cytobiol.

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“…Removal of all teeth and the allowance for ridge healing in the operative site prior to surgery are necessary in the canine model to decrease the rate of infectious complications and prevent soft tissue trauma from chewing. 2 The mandibles of additional types of large animals such as minipigs, 17,18 goats, 19,20 and nonhuman primates 21,22 have been used to examine the effects of boneregeneration materials, because of convenient surgical access and the ability to create large defects. Small rodent animals such as mice, rats, and rabbits have the advantage of being reasonably inexpensive and easier to maintain, permitting studies with sufficient statistical power.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of a rabbit alveolar bone nonhealing defect model

Young

Bashoura

Borden

et al. 2007

J Biomedical Materials Res

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The aim of this study was to develop an easily accessible and reproducible, nonhealing alveolar bone defect in the rabbit mandible. Twenty-four adult male New Zealand white rabbits underwent unilateral mandibular defect surgery. Two types of defect in the premolar/molar region were compared: (1) a 10-mm "full thickness" cylindrical defect removing both cortical plates and the intervening trabecular bone and tooth roots; (2) a 10-mm "partial thickness" cylindrical defect removing only the lateral bony cortex, trabecular bone, and tooth roots. Both types of defect were examined at 0, 8, and 16 weeks using histology and/or microcomputed tomography to determine the quality and quantity of bone formation. The partial thickness defect displayed significant bone fill at 8 weeks (86.9% +/- 10.8%), and complete regeneration of bony contours and bridging by 16 weeks. In contrast, the full thickness defect was never able to bridge itself and displayed no significant difference in bone regeneration between the 8-week (61.5% +/- 3.7%) and 16-week (55.1% +/- 18.5%) time points. These results indicate that a nonhealing defect can be created with a 10-mm bicortical cylindrical ostectomy placed in the premolar/molar region of the rabbit mandible, demonstrating the potential of this animal model as a test bed for mandibular biomaterials and tissue-engineering constructs.

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“…The correct critical sized bone defect to be used in an experimental animal model is important because spontaneous healing progresses quickly if too small a bone defect is chosen. Therefore, we chose a large bone defect animal model to perform our experiment [3][4][5][6][7]9) .…”

Section: Discusstionmentioning

confidence: 99%

Effects of Functionally Graded Hydroxyapatite for Large Mandibular Defects in Adult Rabbits

Yoshimoto

Murata

Akazawa

et al. 2010

J. Hard Tissue Biology.

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Objectives: As an alternative to autograft harvesting, we develop a functionally graded hydroxyapatite (fg-HAp) to induce bone regeneration within extensive bone defects. The functionally graded crystallinity from the inside layer to the surface layer of the fg-HAp promotes bone remodeling and replacement. We evaluate the effects of fg-HAp alone and in combination with fibroblast growth factor (bFGF) on bone regeneration within a bone defect in a rabbit mandible. Methods: The bone defect in the rabbit mandible was 12 x 9 x 3.5 mm in size. For the control group, no material was added to the defect. The experimental groups consisted of fg-HAp alone and fg-HAp combined with 5µg of bFGF. At 2, 4 and 8 weeks after implantation, we excised the mandibles and performed histomorphometrical analysis. Results: The fg-HAp resorption and replacement with new bone occurred fastest at the lingual portion of the defect. The fg-HAp maintained the mandibular outline and was absorbed by multinucleated giant cells. Blood vessel infiltration into the framework of fg-HAp was also observed. Adding 5µg of bFGF did not accelerate bone regeneration. The fg-HAp fragment was observed at the buccal portion of the defect. Excavated bone healing was observed only in the control group. Conclusions: The fg-HAp is an effective substrate for bone regeneration in critically sized bone defects.

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Repair of bone defects by applying biomatrices with and without autologous osteoblasts

Cited by 47 publications

References 13 publications

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

A preliminary study in osteoinduction by a nano-crystalline hydroxyapatite in the mini pig.

Development and characterization of a rabbit alveolar bone nonhealing defect model

Effects of Functionally Graded Hydroxyapatite for Large Mandibular Defects in Adult Rabbits

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