Relationship Between Ultrastructure and Response to Oxytocin of the Mammary Myoepithelium Throughout Pregnancy and Lactation: Effect of Estrogen and Progesterone

Sala, Néstor L.; Freire, Francisco Navarro

doi:10.1095/biolreprod11.1.7

Cited by 29 publications

(12 citation statements)

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“…We suppose that a different oestrogen content in the culture medium (related to different FCS concentrations) might be partly responsible for the discrepancy. Steroid hormones are known to enhance the sensitivity of target cells to OT, possibly by modulating the expression of OT receptors [14,20]. Therefore, high E 2 levels in the culture medium could increase the number of OT binding sites in MCF7 and T47D cells, amplifying the effect of the peptide on cell growth.…”

Section: Discussionmentioning

confidence: 99%

Oxytocin inhibits proliferation of human breast cancer cell lines

Cassoni

Sapino

Negro

et al. 1994

Vichows Archiv A Pathol Anat

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In this study we show that treatment of MDA-MB231 hormone-independent human breast cancer cells with oxytocin (OT) or with the OT analogue F314 induces significant growth inhibition together with a change in cell phenotype. In MCF7 and T47D human breast cancer cells, OT inhibits oestrogen-induced cell growth. In these same cells, OT administration significantly enhances the inhibitory effect of tamoxifen on cell proliferation. MDA-MB231, MCF7 and T47D cells all express mRNA specific for the OT receptor. These data suggest that it may be possible to inhibit breast cancer growth using OT and OT analogues.

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Section: Discussionmentioning

confidence: 99%

Oxytocin inhibits proliferation of human breast cancer cell lines

Cassoni

Sapino

Negro

et al. 1994

Vichows Archiv A Pathol Anat

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“…MELVYN Why individual cells within a culture required widely different time intervals to traverse their own cycle is not known. This variability has been observed with bacteria t&), algae (2), yeasts (3), and animal cells in culture (4)(5)(6)(7). For example, in cultures of Chinese hamster ovary cells, the majority of cells had cycle durations varying from 14 to 21 hours (4), which is probably not due to genetic differences since it occurs in freshly cloned populations (5).…”

Section: Oxytocin Receptors: Triggers For Parturition and Lactation?mentioning

confidence: 93%

“…For example, in cultures of Chinese hamster ovary cells, the majority of cells had cycle durations varying from 14 to 21 hours (4), which is probably not due to genetic differences since it occurs in freshly cloned populations (5). Most variability occurs in the G1 phase of the cell cycle (6)(7)(8), as does the major regulatory event (7,9); G, variability and cell-cycle control, are probably related.…”

Section: Oxytocin Receptors: Triggers For Parturition and Lactation?mentioning

confidence: 99%

Oxytocin Receptors: Triggers for Parturition and Lactation?

Soloff

Alexandrová

Fernström

1979

Science

396

168

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Specific binding of tritiated oxytocin to uterine receptors of pregnant rats increases dramatically at term and is maximal during labor. In mammary glands the increase in binding is gradual, reaching a maximum during the lactation period. Concomitant changes in the sensitivity of the uterus and mammary gland to oxytocin indicate that the receptor concentration is of functional significance. Oxytocin receptors, therefore, may regulate the response of the target organs to circulating oxytocin and thereby control the onset of labor and lactation. Ovarian steroids participate in the regulation of oxytocin receptors in a manner as yet unclarified.

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“…Vimentin expression in vitro has also been correlated with increased growth rate of mesothelial cells [4] and as an indicator of regenerating and proliferating lesions in kidney [11]. In the current study, however, the increase in vimentin expression in myoepithelial cells of lactating breast is most likely not associated with increased growth, as proliferation of myoepithelial cells is reported to cease at late pregnancy [37]. Vimentin upregulation may therefore be associated with specific functions that myoepithelial cells perform during lactation, for example, ejection of milk initiated through oxytocin recep-tors [38], increased synthesis of basement membrane which is required for growth and differentiation of secretory cells [45], or transport of metabolites to secretory cells [30].…”

Section: Discussionmentioning

confidence: 51%