Lactation affects expression of intermediate filaments in human breast epithelium

Michalczyk, Agnes; Ackland, M. Leigh; Brown, Robert W.; Collins, John P.

doi:10.1046/j.1432-0436.2001.067001041.x

Cited by 13 publications

(13 citation statements)

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“…This result was consistent with results of studies in women that indicated that SMA expression is not up-regulated in the lactating, relative to the resting mammary gland. 35 Thus SMA, in conjunction with K5, is a reliable marker of mouse mammary basal cells. Unlike those in the human breast, 74 myoepithelial cells in the mouse mammary gland are not heterogeneous with respect to SMA expression.…”

Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 95%

“…This observation contradicted results of studies in humans that K18 expression is slightly increased during lactation. 35 Decreased K8/18 labeling may be the result of severe expansion of the cytoplasm of secretory luminal cells during lactation, with subsequent dilution of the antigen, rather than of an actual decrease of K8/18 expression. The pattern of K8 and K18 expression in the mammary gland could be monitored by engineering mice transgenic for the green fluorescent protein under control of the promoter of one of these keratins.…”

Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 99%

“…4 Cycles of lobuloalveolar development in the adult are separated by periods of involution of the mammary gland. 11,48 These marked changes in the architecture of the mammary gland are associated with changes in keratin expression in humans 2,5,35,62 and in mice. 3,57 These changes in keratin expression during mammary gland development are important because the formation of cancer in humans and in mice is influenced by developmental changes in the mammary gland at the time of oncogenesis.…”

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confidence: 99%

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Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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Abstract. Immunohistochemical analysis using paraffin-embedded specimens is the method of choice to evaluate protein expression at a cellular level while preserving tissue architecture in normal and neoplastic tissues. Current knowledge of the expression of terminal differentiation markers in the mouse mammary gland relies on the evaluation of frozen tissues by use of immunofluorescence. We assessed changes in patterns of expression of terminal differentiation markers throughout the development of the mouse mammary gland in paraffin-embedded tissues. The expression of a-smooth muscle actin (SMA) and keratins (K) 5, 8/18, and 14 was influenced by the development stage of the mammary gland. Expression of K5 and SMA was restricted to basal cells. Keratin 14 was consistently expressed by mammary basal cells, and was detected in scattered luminal cells from 13.5 days after conception through puberty. Labeling for K8/18 of luminal cells was heterogeneous at all times. Heterogeneous expression patterns in luminal cells suggest this layer has cells with a variety of biological functions. The absence of K6 expression at any stage of the development of the mammary gland was confirmed by use of reverse transcriptase-polymerase chain reaction analysis, which indicates that this intermediate filament is not a marker of the mammary gland stem cell. Finally, consistent with results of earlier studies, keratins 1, 10, 13, and 15, and filaggrin, involucrin, and loricrin were not detected at any stage of mammary gland development.

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Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 95%

Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 99%

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confidence: 99%

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Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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“…Second, the proportion of expressed genes designated as epithelial specific by the stromaonly profiles remained unchanged between days 2 and 6 of pregnancy (i.e., at Ϸ 15% of all expressed genes at days 2, 4, and 6 in both KO and WT profiles). Interestingly, keratin 18 protein levels are increased during lactation in the human breast, during which keratin 18 and 8 appear as intracellular aggregates rather than as components of the filamentous network seen in the nulliparous state (Michalczyk et al, 2001). While keratins remain useful markers of epithelial cells, their use to correct for variable epithelial content is called into question by this result.…”

Section: Mammary Transcriptional Response To Prlmentioning

confidence: 99%

Investigation of the Transcriptional Changes Underlying Functional Defects in the Mammary Glands of Prolactin Receptor Knockout Mice

Ormandy

Naylor²,

Harris³

et al. 2003

Recent Progress in Hormone Research

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Knockout (KO) mice have been created that carry null mutations of genes encoding molecules essential for prolactin (PRL) release, PRL, the receptor for prolactin (PRLR), and various members of the receptor's signaling pathway. This allowed an in vivo genetic analysis of the role of PRL in target organ function. In PRLKO and PRLRKO mice, mammary ductal side branching was absent, terminal end bud (TEB)-like structures persisted at the ductal termini well into maturity, and no alveolar buds formed along the ductal tree. Transplants of recombined mammary glands formed from stromal and epithelial elements with and without PRLR showed normal development, while supplementation of progesterone levels in PRLKO animals restored ductal side branching. During pregnancy, PRLR heterozygous animals initially showed normal ductal and alveolar development. However, alveolar development stalled during late pregnancy, preventing successful lactation. This defect could be rescued by the loss of a single allele of the suppressor of cytokine signaling (SOCS) 1 gene. Transplants of recombined glands containing PRLRKO epithelium and wild-type (WT) stroma formed alveolar buds during pregnancy but showed no lobuloalveolar development. Recombinations of WT epithelium and PRLRKO stroma showed normal development, demonstrating that a direct action of the lactogenic hormones is confined to the epithelium, to promote lobuloalveolar development. Transcript profiling of epithelial transplants expressing or not expressing PRLR was used during early pregnancy to investigate the transcriptional response to lactogens underlying this defect. Such profiling has identified a number of genes with well-characterized roles in mammary development, in addition to a number of novel transcripts.

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“…Es típica de epitelios simples. Igualmente ocurre en el caso de humana 38 . Asimismo unas pocas células suprabasales podrían parecer positivas a la reacción, pero no de una forma clara, lo que se debe a su localización y tamaño, así como su diferenciación según la etapa de estudio 39 .…”

Section: Figura 6 Expresión De La Citoqueratina 14 En Glándula Mamarunclassified

El citoesqueleto celular en la glándula mamaria y su aplicación diagnóstica

Pérez¹,

Rodriguez²

2011

Sanid. Mil.

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Sanid. mil. 2011; 67 (2): 92-97; ISSN: 1887-8571 RESUMEN Objetivos: Valorar la importancia de las modificaciones que aparecen en el citoesqueleto celular (en particular a nivel de unos filamentos intermedios denominados queratinas) a lo largo de los cambios que sufre la glándula mamaria, con especial referencia a la lactación. Su expresión será diferente y servirá como método de contraste. Diseño: 40 ratones distribuidos en cinco grupos según la etapa de estudio. El esquema experimental es común para todos ellos. Material y métodos: Análisis inmunohistoquímico de las muestras procedentes de las glándulas mamarias para evidenciar modificaciones a nivel de las citoqueratinas y comparación con otros estudios en diferentes modelos animales. Resultados: Cada citoqueratina presenta una expresión diferente según sea la estirpe celular y el periodo de lactación. Se diferencian mediante el uso de anticuerpos específicos marcados. Conclusiones: La expresión citoqueratínica varía de manera fisiológica y en células tumorales. La detección inmunohistoquímica de citoqueratinas puede servir como método de diagnóstico complementario.PALABRAS CLAVE: Citoesqueleto, filamentos intermedios, citoqueratina, mama, cáncer.The cellular cytoskeleton in the mammary gland and its diagnostic application SUMMARY: Objectives: To assess the importance of the changes that appear into the cytoskeleton (especially referred to some specific intermediate filaments called keratins) through different stages the mammary gland bears, focusing on the lactation. Their expression will be different in pathological conditions and this will be useful for contrasting other procedures. Design: 40 mice distributed into five groups according to the stage of study. The experimental project is the same for everyone. Material and methods: Immunohistochemical analysis from samples obtained from mammary glands in order to show changes into cytokeratin expression and comparison with other researches in different animal models. Results: The expression of every cytokeratin is different according to the cell lineage and the lactation period. Specific antibodies can reveal it. Conclusions: The cytokeratinic pattern changes in a physiological sense and also in malignant processes. Immunohistochemical detection of cytokeratins may be useful as complementary diagnosis.KEYWORDS: Cytoskeleton, intermediate filaments, cytokeratin, mammary gland, cancer. INTRODUCCIÓNEl cáncer de mama es el más frecuente en la población femenina española con una tasa de incidencia de 40-75 casos por cada 100.000 mujeres. Es la primera causa de muerte por tumores en mujeres en España y la segunda en EEUU tras el cáncer de pulmón 1 . El 75% de los casos aparece en mujeres de más de cincuenta años. Es el responsable de casi el 30% de fallecimientos por causa de cáncer, aunque esta cifra tiende a disminuir gracias a su detección precoz y a los nuevos tratamientos. En el caso de varones supone menos del 1% de todos los casos de cáncer de mama, con un 0,1% de mortalidad 2 .Estudios recientes sugier...

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Lactation affects expression of intermediate filaments in human breast epithelium

Cited by 13 publications

References 40 publications

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Investigation of the Transcriptional Changes Underlying Functional Defects in the Mammary Glands of Prolactin Receptor Knockout Mice

El citoesqueleto celular en la glándula mamaria y su aplicación diagnóstica

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