Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius)

Abstract

Plant regeneration has been achieved from long-term cell suspension cultures established from leaf derived callus of tepary bean (Phaseolus acutifolius). The proportion of densely cytoplasmic cells in suspension culture increased when cultured in the L-6 medium with 54 μM NAA and 2 μM KN. Filtration of the cells at each of five consecutive subcultures resulted in the isolation of a plant regenerating cell line (TB 686), which is being maintained in L-6 medium with 4.5 μM 2,4-D and 2.3 μM zeatin. Differentiated green cell aggregates were obtained when cells from maintenance medium were transferred to the same medium with 10 μM BA. Embryo-like structures developed from these aggregates on L-6 medium with 2.3 μM zeatin, 0.69 μM GA3 and 1.5 μM NAA. Plantlets regenerated from these structures when they were cultured on L-6 medium with 7.0 μM NAA and 1.0 μM KN. Plant regeneration from the cell line remained relatively constant for 270 days. Regenerated plants were grown to maturity in the greenhouse.

Factors affecting morphogenesis from immature cotyledons of Phaseolus coccineus L.

“…Organogenesis was observed by Sreedhar & Mehta (1984) using hypocotyls and epicotyls of P. lunatus L. as explants. More recently, highly efficient plant regeneration has been achieved by Kumar et al (1988) by plating on agar medium long-term cell suspension cultures established from leaf-derived callus of tepary bean (P. acutifolius).…”

Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva

“…Among the most important applications are studies related to plantherbivores interactions (Maffei et al 2006(Maffei et al , 2007. To date, Phaseolus species have been regenerated and transformed genetically with limited success (Christou 1997), with most of the efforts being concentrated on P. vulgaris (Franklin et al 1991;Mohamed et al 1993;Cardenas-Avila et al 2000;Svetleva et al 2003;Veltcheva et al 2005;DelgadoSánchez et al 2006), P. acutifolius (Kumar et al 1988;Malik and Saxena 1992;Mohamed et al 1992;Dillen et al 1996;De Clercq et al 2002), P. aureus (Malik and Saxena 1992), P. coccineus (Santalla et al 1998) and P. polyanthus (Zambre et al 2001).…”

Somatic embryogenesis in Mucuna pruriens

“…Embryogenic system offers an ideal tool for in vitro production and selection of transgenic plants (Finer and McMullen, 1991;Christou, 1997). Such methods are available only for few grain legumes such as Glycine max (Finer and Nagasawa, 1988), Vigna aconitifolia (Kumar et al, 1988), Vigna unguiculata (Kulothungan et al, 1995), Cajanus cajan (Anbazhagan and Ganapathi, 1999) Chickpea (Kiran et al, 2005) and Horsegram (Varisai et al, 2004). In majority of these cases, preference for 2,4-D for embryogenic callus induction is well established (Griga et al, 1987;Mohamed et al, 2004;Anbazhagan and Ganapathi, 1999).…”