Callus induction and shoot regeneration of Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva

Abstract

We report the first protocol for callus induction and shoot regeneration for Phaseolus lunatus L. cv. Wonder Bush and cv. Pole Sieva. We used different explants viz., epicotyls, cotyledons and hypocotyls. The medium used was MS basal medium with thidiazuron (0.5 mg l -1 ) and IAA (0.05 mg l -1 ) for the induction of callus followed by BAP (1.0 mg l -1 ) for the induction of shoots. Epicotyl explants showed the fastest response and the highest percentage of shoot regeneration. This protocol opens new biotechnological strategies to transfer economically important genes to this important crop species.

<i>In Vitro</i> Organogenesis of <i>Quisqualis indica </i>Linn.

“…Similar to these results, the addition of TDZ to MS medium resulted to successful calli induction from leaf explants of Echinaceae purpurea [26]. Stimulated callus growth was observed from epicotyl explants of Phaseolus lunatus [27] on MS containing 0.5 mg/L TDZ and 0.05 mg/L IAA. The best calli induction response from root segments of Dorem ammoniacum was reported by Irvani et al [28] on MS medium supplemented with 2.0 mg/L BA and 1.0 mg/L NAA whereas cotyledon explants of Punica granatum [20] showed the highest frequency of callus induction on MS medium supplemented with 21 µM NAA and 9 µM BA.…”

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

“…Callus regeneration induced by a cytokinin alone or in combination with an auxin is reported in several systems including some legumes. This includes Arachis hypogaea (Venkatachalam et al 1999), Hypericum perforatum (Pretto and Santar'em 2000), Vitex negundo (Rani and Nair 2006), Iris ensata (Boltenkov et al 2007), Vigna subterranea (Kone et al 2006), Albizia odoratissima (Rajeswari and Paliwal 2008), and Phaseolus lunatus (Kanchiswamy and Maffei 2008). BA-and IAA-induced callus regeneration was reported in Acacia sinuata (Vengadesan et al 2000), Citrullus lanatus (Chaturvedi and Bhatnagar 2001) and Argyrolobium roseum (Khanna et al 2006).…”