Factors affecting morphogenesis from immature cotyledons of Phaseolus coccineus L.

Abstract

Direct somatic embryogenesis as well as somatic embryogenesis and organogenesis mediated by small glossy calluses were obtained from immature cotyledon explants of bean (P. coccineus) cv Streamline 770 on a modified half-st~rength MS medium (Murashige & Skoog 1962) containing various concentrations of (2-isopentenyl)adenine and 2-naphthoxyacetic acid. Substitution of sucrose with glucose gave, in the range of concentrations tested, the strongest enhancement of the morphogenic process. Further improvement regarding the number of morphogenic cotyledons, the number of regenerations per cotyledon and the quality of the embryos was observed when 2,3,5-triiodobenzoic acid or abscisic acid were added to the medium. After cycles of micropropagation on MS medium plus 4.4/zM 6-benzyladenine and rooting in the absence of growth factors, plantlets were adapted to ex vitro conditions and grown to maturity.

Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers

“…Types of calli were observed different in different orientations of explants such as green, compact and non embryogenic from vertically placed explants and yellowish whitish, friable, glossy and embryogenic callus in horizontally placed explants. Similar reports are there in Phaseolus coccineus (Genga and Allavena 1991). In contrast to our result, somatic embryo formation was observed on green compact hard callus in Desmodium motorium (Devi and Narmathabai 2011).…”

Morphogenic Aspects of Somatic Embryogenesis

“…Sucrose and auxin concentrations have been found to interact with each other [70], with the optimal concentration· of one being dependent on the concentration of the other. Glucose was reported to be superior to sucrose for the induction of somatic embryos of the scarlet runner bean [71]. Galactose and lactose were reported to be superior to sucrose for the induction of somatic embryos from nucellar callus of citrus, while sucrose was more effective than glucose or fructose [72].…”

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

“…In contrast, the present results demonstrated that higher concentrations of cytokinins did not favor embryo induction, but resulted in more callusing. On the other hand, somatic embryogenesis was effective with glucose in Phaseolus coccineus (Genga and Allavena 1991); maltose in Alfalfa sp. (Strickland et al 1987;Denchev et al 1991) and Glycine max (Finer and McMullen 1991); and the culture maintenance in darkness for many crop species (Thorpe 1988).…”