In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Abstract

In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

“…Though it is well known that the concentration of auxin to cytokinin has a relative effect in the shoot organogenesis [39] in the present study the presence of auxin in the regeneration media decreased the efficiency of shoot induction by BAP which was observed when used alone. The above results were similar to a study in pigeon pea where 2.5mg/L BAP induced higher regeneration of shoot buds but addition of NAA and IBA reduced the regeneration capacity of shoots [40]. In the present study though the shooting response was low in media with BAP and IBA compared to media devoid of IBA but the shoots formed early and they were complete and elongated compared to those formed in BAP.…”

<i>In vitro </i>Regeneration of pigeon pea using leaf explants

“…Supplementing MS medium with 0.5 mg l -1 IBA was optimal in the induction of roots for the in vitro regenerated KAT 60/8 and ICEAP 00557 shoots pigeon. Interestingly there are different reports indicating that IBA at 0.5 mg l -1 was also optimal for other pigeon pea genotypes, namely JKR105, VBN1, VBN2 and SA1 (Franklin et al, 2000;Krishna et al, 2011b). There are also contradicting reports indicating that IBA at 0.2 mg l -1 IBA and 1.0 mg l -1 were optimal for root induction in pigeon pea cultivars Hyderabad C and LGG-29, respectively (Geetha et al, 1998;Prasad et al, 2011).…”

Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

“…RNAi can also be employed in the development of transgenic pigeonpea resistant to PPSMV. However, this will require significant progress in tissue culture and transformation technologies to develop SMD‐resistant transgenics (Krishna et al ., , ).…”