<i>Pigeonpea sterility mosaic virus</i>: a legume‐infecting <i>Emaravirus</i> from <scp>S</scp>outh <scp>A</scp>sia

Patil, Basavaprabhu L.; Kumar, P. Lava

doi:10.1111/mpp.12238

Cited by 41 publications

(29 citation statements)

References 48 publications

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“…Genomic characterization of RRV isolates from Florida indicated the presence of two novel RNAs named RNA5 and RNA6, in addition to the previously described four genomic RNA segments-RNA1, RNA2, RNA3, and RNA4 (Laney et al, 2011). Th e presence of multiple genomic RNAs for other emaraviruses including FMV, RLBV, WMoV and PPSMV has also been previously reported (Elbeaino et al, 2012(Elbeaino et al, , 2014Ishikawa et al, 2012;Kumar et al, 2003;McGavin et al, 2012;Patil and Kumar, 2015;Tantineni et al, 2014). Th e presence of the two novel RNAs from RRV was strongly supported by several factors: a) the two RNAs were found in all ten isolates examined and were present only in the RRV infected samples, b) the two genomic RNAs were highly concordant to the other reported RRV RNAs in their structural organization in that they contained a single ORF in the vcRNA, fl anked by the 5' and 3' UTR region, c) similar to the other genomic RNAs of RRV, RNA5 and RNA6 also contained conserved 5' and 3' terminal inverted complementary13 nucleotide long stretches with two nucleotide mismatches, as also reported for other emaraviruses (Elbeaino et al, 2009;McGavin et al, 2012;Muehlbach, 2007, 2012).…”

Section: Discussionsupporting

confidence: 53%

“…Among the putative members of the genus Emaravirus, FMV possess two additional genomic RNA segments, RNA5 and RNA6, in addition to the RNA1, 2, 3 and 4 (Elbeaino et al, 2012;Ishikawa et al, 2012), which encode complementary sense genes for proteins (known as p5 and p6, respectively) of unknown functions. Th e genome of pigeonpea sterility mosaic virus (PPSMV) consists of fi ve RNAs (Elbeaino et al, 2014;Kumar et al, 2003;Patil and Kumar, 2015) and, possibly, additional genomic segments, but has no experimental evidence. Th e RLBV possesses a fi ft h RNA segment RNA5 (encoding p5 protein) of unknown function (McGavin et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of two novel genomic RNA segments RNA5 and RNA6 in rose rosette virus infecting roses

Babu¹,

Washburn²,

Poduch³

et al. 2016

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Rose rosette virus (RRV), a negative-strand RNA virus belonging to the genus Emaravirus, has recently been characterized to be the causal agent of rose rosette disease. Roses showing typical symptoms of RRV collected from a rose nursery in Florida were subjected to reverse transcription-PCR (RT-PCR) assay using primers corresponding to the conserved inverted 13 nucleotide long stretches found at the termini of the RRV genomic RNA segments. RT-PCR analysis yielded two novel genomic RNA segments, RNA5 and RNA6, in addition to the previously identifi ed four RNA segments. Th e RNA5 is 1650 bp long and encodes for a polypeptide of 465 amino acids (54.3 K), while RNA6 is 1400 bp long and encodes for a polypeptide of 233 amino acids (27.05 K). RACE analysis showed that, both the RNA segments posses at their 5' and 3' termini, stretches of conserved inverted complementary13 nucleotides long sequence with two nucleotide mismatches as previously identifi ed in other genomic RNA segments. Northern blot analysis as well as RT-PCR using specifi c primers showed the presence of the novel genomic RNA segments in infected plants, but absent in the non-infected plants. Th e GenBank Acc. Nos. for the sequences reported in this paper are KT007556 and KT007557.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Identification and characterization of two novel genomic RNA segments RNA5 and RNA6 in rose rosette virus infecting roses

Babu¹,

Washburn²,

Poduch³

et al. 2016

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“…Two emaravirus species, Pigeonpea sterility mosaic emaravirus 1 (PPSMV-1) and Pigeonpea sterility mosaic emaravirus 2 (PPSMV-2), are associated with sterility mosaic disease (SMD) of pigeonpea ( Cajanus cajan (L.) Millsp) [ 1 , 2 , 3 ]. Sterility mosaic disease is one of the major constraints for cultivation of pigeonpea in India and is endemic to India, Nepal, Bangladesh and Myanmar [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The available sequences of PPSMV-1 and PPSMV-2 are from Patancheru (Greater Hyderabad) in the state of Telangana, India. However, SMD in India was also reported from Andhra Pradesh, Bihar, Gujarat, Karnataka, Maharashtra, Tamil Nadu, Telangana and Uttar Pradesh states ( Supplementary Figure S2 ) [ 3 , 8 ]. RNA viruses are highly heterogeneous in nature, because of their huge population sizes, short multiplication time, and high rates of mutation as a result of error-prone replication by the RdRp [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment

Patil

Dangwal

Mishra

2017

Viruses

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Sterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), with genomes consisting of five and six negative-sense RNA segments, respectively. The recently published genome sequences of both PPSMV-1 and PPSMV-2 are from a single location, Patancheru from the state of Telangana in India. However, here we present the first report of sequence variability among 23 isolates of PPSMV-1 and PPSMV-2, collected from ten locations representing six states of India. Both PPSMV-1 and PPSMV-2 are shown to be present across India and to exhibit considerable sequence variability. Variability of RNA3 sequences was higher than the RNA4 sequences for both PPSMV-1 and PPSMV-2. Additionally, the sixth RNA segment (RNA6), previously reported to be associated with only PPSMV-2, is also associated with isolates of PPSMV-1. Multiplex reverse transcription PCR (RT-PCR) analyses show that PPSMV-1 and PPSMV-2 frequently occur as mixed infections. Further sequence analyses indicated the presence of reassortment of RNA4 between isolates of PPSMV-1 and PPSMV-2.

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“…In Northern Nigeria, a strong fiber is extracted from the peduncles of a variety of cowpea. 2 Virus infection usually brings about drastic changes in many physiological processes such changes were mainly studied with the vegetative parts of the host. Alteration due to virus infection in leguminous fruits received very little attention.…”

Section: Introductionmentioning

confidence: 99%

Study on Effect of Cabmv Infection on the Changes in Chemical Composition of Cowpea Cv. Pusa Dofasali

YADAV¹,

KUMAR²

2019

Int J Life Sci Pharm Res

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The total nitrogen protein, nitrate nitrogen, nitrite nitrogen, and total free amino acids are higher in CABMV infected cowpea cv. Pusa Dofasali plant parts i.e. leaves, stems and roots than their healthy counterparts. However, the ammoniacal nitrogen was lower in infected plant parts than the healthy ones. The maximum amounts of total nitrogen, protein and total free amino acids was recorded in diseased leaves, nitrate nitrogen, and nitrite nitrogen in diseased roots and ammoniacal nitrogen in healthy leaves. The minimum amounts of total nitrogen, protein, nitrate nitrogen, nitrite nitrogen, ammoniacal nitrogen, and total free amino acids were present in the stem. Generally, nitrogenous fractions showed an increase up to 80 th day of inoculation and afterwards, their concentration decreased. The concentration of total nitrogen, protein, nitrate nitrogen, nitrite nitrogen and total free amino acids was higher in diseased seed but ammoniacal nitrogen was higher in healthy seeds than diseased ones. An increased concentration of nitrogenous fraction in the virus infected plant parts of cowpea was observed. Unless previously fixed nitrogen was effectively used (or) transported elsewhere, the presence of excess nitrogenous compounds could alter the C/N ratio, which might inhibit the normal rate of nitrogen fixation. Virus infection usually brings about drastic changes in many physiological processes such changes were mainly studied with the vegetative parts of the host. Alteration due to virus infection in leguminous fruits received very little attention. Therefore, it was planned to study the effect of CABMV infection on the chemical composition of the cowpea cv. Pusa Dofasali fruits.

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Pigeonpea sterility mosaic virus: a legume‐infecting Emaravirus from South Asia

Cited by 41 publications

References 48 publications

Identification and characterization of two novel genomic RNA segments RNA5 and RNA6 in rose rosette virus infecting roses

Identification and characterization of two novel genomic RNA segments RNA5 and RNA6 in rose rosette virus infecting roses

Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment

Study on Effect of Cabmv Infection on the Changes in Chemical Composition of Cowpea Cv. Pusa Dofasali

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