Somatic embryogenesis in Mucuna pruriens

S, Harini; Sathyanarayana, N.

doi:10.5897/ajb09.815

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…Two regeneration systems were developed in cowpea, from shoot apices of 3-5-day-old seedlings (Mao et al, 2006), and via embryonic axes explants (Yusuf et al, 2008). Harini and Sathyanarayana (2009) reported the induction of somatic embryos in Mucuna pruriens belongs to the family Fabaceae from different explants cultured on MS medium with 11.31 µM 2,4-D produced embryogenic callus. Optimizing factors for T-DNA delivery with transformation efficiency more than 20% resulted in kanamycin-resistant transgenic shoots of Thlaspi caerulescens L. using Agrobacterium EHA105 harboring pBI121 with the nptII and the gusA genes were described by Safavi and Asgari (2011).…”

Section: Kanamycin Selectionmentioning

confidence: 99%

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

Eissa¹

2013

Egyptian Journal of Genetics and Cytology

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Section: Kanamycin Selectionmentioning

confidence: 99%

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

Eissa¹

2013

Egyptian Journal of Genetics and Cytology

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“…Various physicochemical parameters which are mentioned below were performed to establish quality of the prepared formulations. (12)(13)(14)(15)(16)(17)(18)(19))…”

Section: Evaluation Of Studies For Prepared Formulationsmentioning

confidence: 99%

“…The measurement pH of formulation was done in previously calibrated pH meter. (12)(13)(14)(15)(16)(17)(18)(19) Irritancy test: The irritancy test was performed on the herbal formulation .There is no redness or irritancy in the preparation .The condition was seen for 24 hrs.…”

Section: Determination Of Ph Testmentioning

confidence: 99%

Formulation and Evaluation of Harbal Mouthwash

2023

IJPREMS

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The goal of this study is to develop a formula and test its efficacy against bacteria found in the oral cavity. The goal of this study is to develop and test herbal mouthwash, as well as to determine its efficacy against oral microbial load. Water was removed from the plant materials that were collected. the plant components were collected and watersoluble compounds were removed. The antibacterial activity and physicochemical properties of prepared mouthwash were also tested. The present mouthwash possesses a good antibacterial property. The findings of the stability research back up the effectiveness of the preparation. Mouthwash is currently a liquid preparation that usually comprises antibacterial and antiseptic ingredients. These treatments can be used to limit microbial growth in the mouth, but they can also be utilised for other reasons, such as analgesic, anti-inflammatory, or anti-fungal properties. The ingredient contains turmeric, clove oil, honey and Peppermint oil were chosen for mouthwash, and the final formulation was tested for antimicrobial activity against Staphylococcus aureus and S. salivarius cultures, as well as physical properties such as pH, colour, and stability. The presence of mouthwash has a strong antibacterial effect. Mouthwash is a liquid preparation that generally contains antibacterial and antiseptic compounds, and it is stable in a variety of temperatures. This solution can be used to minimise microbial growth in the mouth, as well as for other purposes such as analgesic, anti-inflammatory, or antifungal activities.

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“…[26], Mucuna pruriens (L.) DC. [27], and Robinia pseudoacacia L. [22] have established tissue culture systems.…”

Section: Introductionmentioning

confidence: 99%

Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum

Qiao,

Jin,

Liu

et al. 2023

Plants

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Eremosparton songoricum (Litv.) Vass. is a rare and extremely drought-tolerant legume shrub that is distributed in Central Asia. E. songoricum naturally grows on bare sand and can tolerate multiple extreme environmental conditions. It is a valuable and important plant resource for desertification prevention and environmental protection, as well as a good material for the exploration of stress tolerance mechanisms and excellent tolerant gene mining. However, the regeneration system for E. songoricum has not yet been established, which markedly limits the conservation and utilization of this endangered and valuable desert legume. Assimilated branches derived from seedlings were cultured on several MS mediums supplemented with various concentrations of TDZ or 6-BA in different combinations with NAA. The results showed that the most efficient multiplication medium was MS medium supplemented with 0.4 mg/L 6-BA and 0.1 mg/L NAA. The most efficient rooting medium was WPM + 25 g/L sucrose. The highest survival rate (77.8%) of transplantation was achieved when the ratio of sand to vermiculite was 1:1. In addition, the optimal callus induction medium was MS + 30 g/L sucrose + 2 mg/L TDZ + 0.5 mg/L NAA in darkness. The E. songoricum callus treated with 100 mM NaCl and 300 mM mannitol on MS medium could be used in proper salt and drought stress treatments in subsequent gene function tests. A rapid and efficient regeneration system for E. songoricum that allowed regeneration within 3 months was developed. The protocol will contribute to the conservation and utilization of this rare and endangered desert stress-tolerant species and also provide a fundamental basis for gene functional analysis in E. songoricum.

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Somatic embryogenesis in Mucuna pruriens

Cited by 5 publications

References 24 publications

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

Formulation and Evaluation of Harbal Mouthwash

Establishment of an Efficient and Rapid Regeneration System for a Rare Shrubby Desert Legume Eremosparton songoricum

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