Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory ResponseIn Vitro

Abstract

Silica inhalation can induce chronic lung inflammation and fibrosis. Upon silica stimulation, activated macrophages trigger the T-lymphocyte which can differentiate into many different types of Th cells, including the recently discovered Th17 cells. IL-17A, the typical Th17 cytokine, is reported in some inflammatory diseases. However, the role of IL-17A in silica-induced inflammatory response is still not clear. The regulatory mechanism of silica-induced Th17 response also needs to be investigated. So we established a mice primary cell coculture system (macrophage and lymphocyte) to investigate the role of IL-17A in silica-induced inflammatory response in vitro, by using anti-IL-17A mAb and IL-1Ra. Both anti-IL-17A mAb and IL-1Ra decreased the level of IL-17A and increased the function of Treg cells. The Th1 response was suppressed and the Th2 response was promoted by the addition of anti-IL-17A mAb or IL-1Ra. IL-1Ra treatment decreased the level of IL-6, whereas the levels of IL-23 and ROR-γt were increased. Our study demonstrated that IL-17A reduction altered the pattern of silica-induced Th responses by boosting the function of Treg cells in vitro. Blocking the function of IL-1 signal pathway could suppress the level of IL-17A, which played the major role in modulating silica-induced Th responses in vitro.

IL-10 producing B cells regulated 1,3-β-glucan induced Th responses in coordinated with Treg

“…Cell treatment C57BL/6 mice were randomly allocated into 2 groups according to weight: antiCD22 group and vehicle group as needed. Macrophages were isolated from vehicle group mice intraperitoneally, and puri ed by a 2 hrs adherence culture as previous described [26]. CD4 + T cells were rst puri ed from vehicle group mice spleen by MACS, and then Treg(CD4 + CD25 + ) and Teff (CD4 + CD25 − )were separately isolated by positive selection and negative selection according to a magnetic column-based system Treg/Teff cell isolation kit (Miltenyi Biotech, Auburn, CA).…”

Role of IL-10-producing regulatory B cells in modulating T-helper cell immune responses during silica-induced lung inflammation and fibrosis

“…The Th17 response also is involved in the initial stage of silicosis. IL-17 neutralization can influence the traditional Th1/Th2 immune balance after silica instillation 4 5 6 . We previously demonstrated that CD4 + Foxp3 + Treg was involved in modulation of Th immune balance after silica instillation.…”

Anti-influenza virus effects of crude phenylethanoid glycosides isolated from ligustrum purpurascens via inducing endogenous interferon-γ

“…In addition, significant changes in CD3 + count and ratio of CD4 + / CD8 + change in spleen were observed during viral infection Tang et al, 2014;Yang et al, 2013). We found that H1N1 inhibit the generation of CD3 + T cells and disturb the differentiation of T-lymphocyte subsets (Fig.…”