Role of IL-10-producing regulatory B cells in modulating T-helper cell immune responses during silica-induced lung inflammation and fibrosis

Abstract

Silicosis is characterized by chronic lung inflammation and fibrosis, which are seriously harmful to human health. Previous research demonstrated that uncontrolled T-helper (Th) cell immune responses were involved in the pathogenesis of silicosis. Lymphocytes also are reported to have important roles. Existing studies on lymphocyte regulation of Th immune responses were limited to T cells, such as the regulatory T (Treg) cell, which could negatively regulate inflammation and promote the process of silicosis. However, other regulatory subsets in silicosis have not been investigated in detail, and the mechanism of immune homeostasis modulation needs further exploration. Another regulatory lymphocyte, the regulatory B cell, has recently drawn increasing attention. In this study, we comprehensively showed the role of IL-10-producing regulatory B cell (B10) in a silicosis model of mice. B10 was inducible by silica instillation. Insufficient B10 amplified inflammation and attenuated lung fibrosis by promoting the Th1 immune response. Insufficient B10 clearly inhibited Treg and decreased the level of IL-10. Our study indicated that B10 could control lung inflammation and exacerbate lung fibrosis by inhibiting Th1 response and modulating the Th balance. The regulatory function of B10 could be associated with Treg induction and IL-10 secretion.

Histone deacetylase 11 inhibits interleukin 10 in B cells of subjects with allergic rhinitis

“…Apart from producing immunoglobulins (Igs), B cells also produce immune regulatory molecules, such as interleukin (IL)‐10, transforming growth factor (TGF)‐beta, and thrombospondin‐1 . These immune regulatory molecules confer B cells’ ability to maintain immune homeostasis in the body . Although it is known that the numbers or functions of these immune regulatory B cells are compromised in immune diseases, the underlying mechanisms need to be further investigated.…”

Immune Alteration Caused by Fibrous and Particulate Environmental Substances

“…Then, they suggested that Wnt/β-catenin pathway regulates Treg and contributes to fibrogenesis in silicosis. Additionally, other than Th17, Liu et al reported the role of IL-10-producing regulatory B cell in silicosis, since this B cell increased in silica instillation in mouse model [66]. The produced IL-10 seemed to suppress Th1 response with IL-10 produced from Treg.…”

Human monocytic myeloid‐derived suppressor cells impair B‐cell phenotype and function in vitro

“…The downregulation of IL-10 transcription in B cells upon M-MDSC co-culture is of utmost interest, as IL-10 + regulatory B cells have been shown to be important mediators of T-cell responses [54]. When analyzing the rate of CD19 + IL-10 + cells using intracellular flow cytometry staining, we found slightly but not significantly reduced proportions of IL-10-expressing B cells in the co-culture of M-MDSCs (Supporting Information Fig.…”