IL-10 producing B cells regulated 1,3-β-glucan induced Th responses in coordinated with Treg

Abstract

Background: Repeated exposure to fungi contaminated dust can lead to multiple adverse effect on lung, such as hypersensitivity pneumonitis, granuloma even irreversible brosis. 1,3-β-glucan, as the major cell wall component of fungi, was considered as its exposure biomarker. Existing studies showed that series of Th response were involved in 1,3-β-glucan induced hypersensitivity pneumonitis, in which macrophages, regulatory T cells (Treg) and IL-10 producing B cells were reported to participate. The reciprocal interaction among those critical immune cells in 1,3-β-glucan induced in ammation were not investigated yet.Results: To clarify the regulatory mechanism of IL-10 producing B cells on Th and Treg, current study set up a primary cell co-culture system. Anti-CD22 antibody was injected intraperitoneally to generate IL-10 producing B cells de ciency mouse model. Cells were isolated and puri ed from different groups' mice.Flow cytometry was used to check the phenotype of different cell subtypes. CBA assay and realtime PCR were used to examine the levels of multiple cytokines. Our results indicated that IL-10 producing B cells were involved in modulating 1,3-β-glucan induced in ammatory response. The modulation of IL-10 producing B cells on Th response after 1,3-β-glucan treatment was independent on cell-cell contact.What's more, the modulation pattern of IL-10 producing B cells might be impaired without Treg response.Conclusions: IL-10-producing B cells regulated 1,3-β-glucan induced Th responses in coordinated with Treg.

Immunomodulatory Effect of Sasa quelpaertensis Leaves Fermentation Products in Mice

“…To elucidate the regulatory mechanism of IL-10 producing B cells in Th and Treg, an IL-10 producing B cell deficient mouse model was generated by intraperitoneal injection of anti-CD22 antibody. The results confirmed that IL-10 producing B cells can modulate the 1,3-β-glucan-induced inflammatory response [40].…”