Reduced natural killer cell activity and IL-2 production in malnourished cancer patients

Villa, Maria Luisa; Ferrario, E.; Bergamasco, Ellen Cristina; Bozzetti, Federico; Cozzaglio, Luca

doi:10.1038/bjc.1991.219

Cited by 21 publications

(8 citation statements)

References 15 publications

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“…It remains unclear whether the reduced allogeneic IL-7-induced LAK activity in three of 14 patients can be attributed to a general or specific immunosuppression or whether it reflects normal interindividual variations in the inducibility of LAK cells. Reduced natural killer (NK)-cell activity has been reported in patients with advanced cancer (Morikawa et al, 1989;Villa et al, 1991). On the other hand, we found striking interindividual differences in the degree of IL-7-induced LAK cell-activity even among healthy volunteers (Schadendorf et al, 1994a).…”

Section: Discussionmentioning

confidence: 33%

Lysis of allogeneic and autologous melanoma cells by IL-7-induced lymphokine-activated killer cells

Böhm

Möller²,

Kalbfleisch³

et al. 1994

Br J Cancer

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SmmuaryIn order to assess the potential of interleukin 7 (IL-7) as an immunotherapeutic agent in human melanoma, we have evaluated the in vitro activity of IL-7-induced lymphokine-activated killer (LAK) cells from patients with advanced melanoma against allogeneic and autologous melanoma cells. Peripheral blood lymphocytes (PBLs) from 14 patients with stage III melanoma were isolated and incubated in the presence of 1,000 U ml-' IL-7 and 1 U ml-' IL-2 for comparison. LAK-ell activity was determined by a 24 h cytotoxicity assay using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. The activity of IL-7-induced LAK cells against two allogeneic melanoma cell lines was 32.7% (± 17.9) against SK-Mel-37 and 38.1% (± 12.5) against SK-Mel-23 at an effector-to-target (En ratio of 20:1. The activity of IL-2-induced LAK cells was significantly higher against SK-Mel-37 (78 ± 24.6%) and against SK-Mel-23 (73.5 ± 19.7%). IL-7 and suboptimal doses of IL-2 (10 U ml-I) were found to have a co-stimulatory on lymphocyte proliferation as well as on LAK activity. Against autologous melanoma cells, the activity of IL-7-and IL-2-induced LAK cells did not differ significantly (55.8 ± 25.6% versus 68.7 ± 21.7% respectively). In two patients, IL-7-induced LAK-cell activity against autologous melanoma cells exceeded even that of IL-2 significantly (67% vs 35% and 95% vs 82%). Levels of tumour necrosis factor a (TNF-x) in the supernatants of LAK-cell cultures generated by IL-7 were lower than those of IL-2-generated LAK-cell cultures. These results suggest that IL-7 is a potential alternative to immunotherapy with IL-2 in terms of efficacy and possible side-effects and encourages pilot studies with IL-7 in melanoma patients.

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Section: Discussionmentioning

confidence: 33%

Lysis of allogeneic and autologous melanoma cells by IL-7-induced lymphokine-activated killer cells

Böhm

Möller²,

Kalbfleisch³

et al. 1994

Br J Cancer

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“…In addition, serum albumin concentrations were 2.5-3.5 g/dL and BMIs were around 18 kg/m 2 . Because malnutrition and aging have been shown to impair innate immune functions [17][18][19][20][25][26][27][28][29], NK cells and neutrophils in our study population could have been exhausted. Furthermore, it should be noted that serum CRP concentrations were above normal even at the beginning of the study, and persisted throughout.…”

Section: Discussionmentioning

confidence: 78%

Long-Term Enteral Immunonutrition Containing Lactoferrin in Tube-Fed Bedridden Patients: Immunological and Nutritional Status

Takeuchi¹,

Yamamura²,

Takahashi³

et al. 2012

Journal of the American College of Nutrition

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“…The activity of NK cells in cancer patients was lower than that in normal subjects [29], and the number of CD3 − CD16/56 + cells as NK cells in our patients was also lower compared with healthy subjects. These changes may be related with malnutrition rather than lung cancer because correction of hypoproteinemia corrected the depressed NK activity quickly in malnourished cancer patients [29]. On the other hand, our results showed a significantly high proportion of ␥␦ T cells in patients compared with controls.…”

Section: Discussionmentioning

confidence: 92%

“…Because malnourished hospitalized adult patients and cancer patients are also known to have a significantly low total lymphocyte count and cellular immunity or natural killer (NK) activity [3,29], we also examined the nutritional status in our subjects by using Niederman's nutritional index [21] to evaluate its relationship to immune function.…”

Section: Introductionmentioning

confidence: 99%

Immunological Competence and Nutritional Status in Patients with Lung Cancer

Shirai

Kadota

Iida

et al. 1998

Lung

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The rate of infection in patients with malignant disease is significantly higher than in patients with benign disease. To investigate whether immunological competence is impaired in patients with lung cancer, we assessed neutrophil function (chemotaxis, phagocytosis, bacterial killing activity, and superoxide production), monocyte function (phagocytosis and killing activity), lymphocyte subsets using flow cytometry, and proliferation of lymphocytes stimulated by phytohemagglutinin, concanavalin A, and pokeweed mitogen. Studies were performed on 22 untreated patients with lung cancer and 21 age-matched healthy volunteers. Nutritional status was assessed by Niederman's nutritional index. In patients with lung cancer neutrophil chemotaxis, monocyte phagocytosis and killing, proliferation of lymphocytes stimulated by phytohemagglutinin and concanavalin A, but not pokeweed mitogen, and the number of natural killer cells were significantly lower than in healthy volunteers, whereas gammadelta T cells were increased (p < 0.05). The mean score on Niederman's nutritional index was worse in patients than in healthy volunteers (p < 0.001). Our results suggest that the impaired immunological competence and undernutrition may be among the mechanisms causing increased susceptibility to infection in patients with lung cancer.

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Reduced natural killer cell activity and IL-2 production in malnourished cancer patients

Cited by 21 publications

References 15 publications

Lysis of allogeneic and autologous melanoma cells by IL-7-induced lymphokine-activated killer cells

Lysis of allogeneic and autologous melanoma cells by IL-7-induced lymphokine-activated killer cells

Long-Term Enteral Immunonutrition Containing Lactoferrin in Tube-Fed Bedridden Patients: Immunological and Nutritional Status

Immunological Competence and Nutritional Status in Patients with Lung Cancer

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