Reconstruction of goat femur segmental defects using triphasic ceramic-coated hydroxyapatite in combination with autologous cells and platelet-rich plasma

Nair, Manitha B.; Varma, H. K.; Menon, K. Venugopal; Shenoy, Sachin J.; John, Annie

doi:10.1016/j.actbio.2009.01.009

Cited by 62 publications

(70 citation statements)

References 43 publications

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“…Therefore, most studies of fish scale waste have focused on isolating and characterizing collagen [5,[7][8][9]. In recent years, hydroxyapatite has attracted interest for use in bone grafting because of their osteoconductive and bioactive properties [10,11]. Other medical applications of hydroxyapatite have also been studied because hydroxyapatite is biocompatible, bioactive, non-toxic, non-inflammatory and non-immunogenic [12,13].…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Huang

Hsiao

Chai

2011

Ceramics International

155

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Section: Introductionmentioning

confidence: 99%

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Huang

Hsiao

Chai

2011

Ceramics International

155

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“…In orthopedic research, MSCs from goat [6][7][8][9][10][11], dog [12][13][14][15], sheep [16][17][18][19], and pig [20,21] are studied with the aim to correct defects of cartilage and bone. In cardiac research MSCs from dog [22][23][24], pig [25,26], and sheep [27,28] are studied for heart valve reconstruction and for cellular therapy to treat myocardial infarctions.…”

Section: Introductionmentioning

confidence: 99%

Prospective Isolation of Mesenchymal Stem Cells from Multiple Mammalian Species Using Cross-Reacting Anti-Human Monoclonal Antibodies

Rozemuller

Prins

Naaijkens

et al. 2010

Stem Cells and Development

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Mesenchymal stem cells (MSCs) of human and nonhuman mammalian species are often studied for various applications in regenerative medicine research. These MSCs can be derived from human bone marrow (BM) and identified by their ability to form fibroblast-like colony forming units that develop into stromal like cells when expanded in culture. These cells are characterized by their spindle-shaped morphology, their characteristic phenotype (CD73 + , CD90 + , CD105 + , CD45À , and CD34 À ), and their ability to differentiate into cells of the osteogenic, adipogenic, and chondrogenic lineages. However, the identification and purification of MSCs from nonhuman mammalian species is hampered by the lack of suitable monoclonal antibodies (mAb). In this report, primary BM and cultured BM-derived MSCs of human and monkey, goat, sheep, dog, and pig were screened for cross-reactivity using a panel of 43 mAb, of which 22 react with either human BM mononuclear cells or cultured human MSCs. We found 7 mAb with specificity for CD271, MSCA-1 (W8B2 antigen), W4A5, CD56, W3C4 (CD349), W5C4, and 58B1, which showed interspecies cross-reactivity. These mAb proved to be useful for prospective sorting of MSCs from the BM of the 6 mammalian species studied as well as for the characterization of their cultured offspring. Flow sorting with the cross-reacting mAb resulted in up to 2400-fold enrichment of the clonogenic cell fraction (fibroblast-like colony forming units). This study provides an important contribution for the comparative prospective isolation of primary BM-MSCs and the characterization of cultured MSCs from multiple mammalian species for preclinical research.

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“…Previous in vitro degradation studies (as per ISO standard 10993-13) proved that there is a phase change in HASi (TCP and calcium silicate phase present in the outer coated layer of HASi dissoluted indicating degradative nature) when immersed in PBS at 378C for 60 days, whereas HA did not show any change in its characteristic phase. 13 The results from in vitro studies have prompted to use HASi as a bone substitute for segmental defect studies, in which the performance of bare HASi (without cells or platelet-rich 18 The results in those studies demonstrated that new bone formation and its mineralization occurred faster throughout the defect, in par with material degradation in tissue engineered (TE) groups. In bare HASi groups, mature lamellar bone was seen only in the peripheral region of defect, whereas in the mid region newly formed trabeculae were scanty and most of the area was occupied with material together with the infiltration of osteoblast-like cells.…”

Section: Discussionmentioning

confidence: 97%

Treatment of Goat Femur Segmental Defects with Silica-Coated Hydroxyapatite—One-Year Follow-Up

Nair

Varma

Shenoy

et al. 2010

Tissue Engineering Part A

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Segmental bone defects caused by tumor resections, trauma, and skeletal abnormalities such as osteomyelitis remain a major problem in orthopedics because of the lack of predictability in attaining functional bone after the treatment. The objective of this study was to propose an indigenous porous biodegradable triphasic ceramic (calcium silicate, tricalcium phosphate, and hydroxyapatite [HA])-coated HA (core) (HASi) for the repair of such segmental defects. With respect to the synthesis of HASi, HA blocks were prepared by wet precipitation, dipped in silica sol (sol gel method), sintered at 1200 degrees C, polished in the form of hollow cylinder (2 cm long with an outer and inner diameter of 2 cm and 7 mm, respectively), and implanted into a 2-cm segmental defect created in the goat femur diaphysis. This study prolonged for 12 uneventful months and thereafter neo-osteogenesis in par with material degradation was analyzed through radiography, histology, histomorphometry, scanning electron microscope (SEM)-energy dispersive spectrum, micro-computed tomography, and inductively coupled plasma spectrometry. HASi proved to be osteoconductive, osteointegrative, and degradative in nature, without the intervention of fibrous tissue formation at the defect site. Histologically, the newly formed bone reorganized, mineralized, and attained the appearance and contour of the original femoral diaphysis in 1 year. The interconnected porous structure with silica composition aided progressive bone regeneration and repair in par with degradation of the material. Thus the study proposed the possibility of using HASi as a suitable material in clinical orthopedic reconstructive surgery, which remains a formidable challenge.

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Reconstruction of goat femur segmental defects using triphasic ceramic-coated hydroxyapatite in combination with autologous cells and platelet-rich plasma

Cited by 62 publications

References 43 publications

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Prospective Isolation of Mesenchymal Stem Cells from Multiple Mammalian Species Using Cross-Reacting Anti-Human Monoclonal Antibodies

Treatment of Goat Femur Segmental Defects with Silica-Coated Hydroxyapatite—One-Year Follow-Up

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