Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Huang, Yi‐Cheng; Hsiao, Pei-Chi; Chai, Huey-Jine

doi:10.1016/j.ceramint.2011.01.018

Cited by 175 publications

(123 citation statements)

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“…The synthesized CHA nanorods were characterized by various analytical measurements to investigate how changes of carbonate levels affect the crystal morphology and structure of CHA nanorods. The effects of synthesized samples on the viability and osteogenic differentiation of the human osteosarcoma MG-63 cells have been measured by an MTT method and alkaline phosphate activity assay [18,19]. …”

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confidence: 99%

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Xue¹,

Chen²,

Huang³

et al. 2016

Nano Online

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(http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.Keywords: Hydroxyapatite; Carbonated hydroxyapatite; Nanorods; Cell viability; Alkaline phosphatase activity; MG-63 cell BackgroundHydroxyapatite (HA) is the main inorganic component of human bone mineral, and the content of carbonate in human bone mineral is about 5-8 wt % [1, 2]. Carbonate ions in carbonated hydroxyapatites (CHA) substitute both the phosphate and hydroxyl sites of the HA structure and each is called A-type CHA and B-type CHA, respectively. Predominantly, the carbonate ions are present as B-type carbonates in natural bone minerals [3]. Synthetic CHAs have been widely used in a variety of biomedical applications including osteoconductive coatings [4][5][6], bone-substitute biomaterials [7], and vehicles for drug delivery [ 8]. Recently, hydoxyapatite nanorods have been prepared by an ethanol-induced method [9], liquid crystals [10], sonochemical synthesis [11], sol-gel method [12], and hydrothermal reaction [13,14]. However, few methods have been reported for the preparation of carbonated hydroxyapatite nanorods with different carbonate contents. Since carbonate ion substitution in the apaptite lattice plays a major role in the biochemistry and physical properties of biological apatites, it is important to develop convenient ways for the synthesis of CHA nanorods with different carbonate contents and understand how various carbonate contents affect the crystal structure and biocompatibility of CHA nanorods. Table 1 Synthesizing materials for preparing HA and CHA nanorods SamplesCaThe hydrothermal method is a typical process which has been widely used in synthesis of inorganic materials for its good repeatability and crystallinity control [15][16][17]. In this study, we developed a hydrothermal process to synthesize carbonated hydroxyapatite nanorods with different carbonate contents, using ethylene diamine tetraacetic aci...

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confidence: 99%

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Xue¹,

Chen²,

Huang³

et al. 2016

Nano Online

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“…Peaks were obtained at 2θ value of 25.9°, 31.84°, 32.98°, 34.12°, 39.84°, 46.76°, 49.54° corresponding to (002), (211), (300), (202), (310), (222), (213) planes, respectively. Well-resolved characteristic peak of highest intensity was obtained at 2θ value of 31.84° corresponding to 211 plane of HAp (Huang et al, 2011;Panda et al, 2014). Similar to Panda et al (2014), crystallization of the FS-Hap powder has been also confirmed due to sharp peak intensity and well-resolved peaks in XRD pattern.…”

Section: Resultsmentioning

confidence: 51%

“…Fish by-product has tremendous unexploited potential for adding value. However, the use of fish by-product in foods and biochemical products for human consumption is still under intensive study in the aquaculture industry (Huang et al, 2011). Hydroxyapatite (HAP), with a chemical formula of Ca 10 (PO 4 ) 6 (OH) 2 , is one of constituent that had found in either in human bone or teeth and the major elements are including calcium and phosphorous.…”

Section: Introductionmentioning

confidence: 99%

Extraction, Characterization and Antimicrobial Activity of Hydroxyapatite From Seabass and Seabream Scale

Alparslan¹

2017

J Food Health Sci

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Abstract:The present study investigates the characterization of hydroxyapatite (HAp) extracted from seabass and seabream scales as by-product. Fish scales obtained from a seafood processing company were used to extract natural HAp powder. HAp powder was extracted by alkaline heat treatment of fish scales and the synthesized HAp (FS-HAp) was extensively characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. Calcium to phosphate ratio of the HAp was confirmed by inductively coupled plasma (ICP) and elemental analysis of HAp were also carried out using energy dispersive x-ray spectroscopy (EDS). The results of the characterization analysis were compared with commercial hydroxyapatite standard (CHAp) and it was clearly confirmed that the extracted FS-HAp exactly showed CHAp characteristics physicochemically which is used as biomaterial. However, well diffusion assay revealed out that synthesized hydroxyapatite showed no activity against C. albicans, S. aureus and E. coli. It was concluded that, instead of synthetic apatite, extracted FS-HAp presents a potential promising biomaterial as the raw materials are by-product which economically cheap and sustainable substances.

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“…The band at 1650 cm −1 is attributed to the 2 bending mode of the H 2 O molecule (Corami et al, 2007(Corami et al, , 2008bRey et al, 1995). The adsorption band at 2916 cm −1 could be due to a stretching mode of the P OH group (Gómez-Morales et al, 2013;Huang et al, 2011), and a small band at 2365 cm −1 was attributed to potassium bromide (KBr) (Huang et al, 2011). Carbonate group (CO 3 2− ) in the FTIR spectra was identified by intense bands at 875 and 1412-1540 cm −1 produced from asymmetric stretching 2 and 3 (Corami et al, 2008b;Huang et al, 2011).…”

Section: Characterization Of Powdered Fish Scales (Pfs)mentioning

confidence: 95%

Response surface methodology for the elimination of humic substances from water by coagulation using powdered Saddled sea bream scale as coagulant-aid

Harfouchi

Hank

Hellal

2016

Process Safety and Environmental Protection

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Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells

Cited by 175 publications

References 29 publications

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Extraction, Characterization and Antimicrobial Activity of Hydroxyapatite From Seabass and Seabream Scale

Response surface methodology for the elimination of humic substances from water by coagulation using powdered Saddled sea bream scale as coagulant-aid

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