Recent Advances in Electrochemiluminescence Analysis

Li, Lingling; Chen, Ying; Zhu, Jun‐Jie

doi:10.1021/acs.analchem.6b04675

Cited by 475 publications

(297 citation statements)

References 156 publications

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“…Besides for RIAs and ELISAs, electrochemiluminescence (ECL) immunoassays, particularly commercial ECL immuno‐analyzers, have become popular in clinical biolabs due to their high sensitivity and automation. Nevertheless, the commercial ECL clinical instruments require specialized consumables and typically lack user‐configurable ECL measuring setup due to the preconfigured assay menu . Therefore, one can see that besides the indispensable immunoreaction, in most of the developed immunoassays, one or more round of additional signal amplification and transducing reactions are generally required.…”

Section: Figurementioning

confidence: 99%

Single Microbead‐Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens

Chen

Zhang

Zhu

et al. 2017

Chemistry An Asian Journal

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A new concept of single microbead (MB)-anchored fluorescent immunoassay (SMFIA) is proposed with greatly improved sensitivity. In the SMFIA, a single MB is manipulated as the reaction carrier so that the target-tethered fluorescent immunocomplexes will be highly concentrated on one MB. By monitoring the enriched fluorescence signal on the single MB through imaging, highly sensitive target quantification can be realized just by employing the most common sandwich immunoreactions without requirement of further signal amplification routes. The high sensitivity of the SMFIA can fully meet the demand of current medical diagnosis. Furthermore, we have further advanced a fluorescence-encoding mechanism for the proposed SMFIA which allows the simultaneous detection of multiple antigens in a single reaction. Sharing the distinct advantages of simple operation, high sensitivity and multiplexed detection capability, the SMFIA provides a general platform for the detection of various biomarkers.

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Section: Figurementioning

confidence: 99%

Single Microbead‐Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens

Chen

Zhang

Zhu

et al. 2017

Chemistry An Asian Journal

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“…Aside those luminescent materials used in ECL analysis as organic luminophores, transition metal (II, III) complexes, metal clusters, semiconductors and quantum dots etc., luminol is one of the most significant and widely applied reagent in analytical applications . Its cheapness and convenient usage in aqua solution lead to its successful progress as a mature analytical character in the fields of environmental monitoring, foodstuff industry and especially clinical chemistry.…”

Section: Introductionmentioning

confidence: 99%

Progress of the Electrochemiluminescence Biosensing Strategy for Clinical Diagnosis with Luminol as the Sensing Probe

Chen

Yan

et al. 2017

ChemElectroChem

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Electrochemiluminescence (electrogenerated chemiluminescence, ECL), which involves light emission from excited states of electrochemically generated species at the electrode surface, is widely applied for chemical analysis. Integrating the advantages of outstanding selectivity from biological recognition and the high sensitivity of ECL signaling, ECL biosensors are powerful for ultrasensitive bio‐checkups and quantification, in which luminol is adopted as an important luminophore. Nanomaterials have been introduced into ECL biosensors, which enlarge the applicable pH range and improve analytical performances, with successful applications in clinical events. In particular, this Minireview will focus on new progresses related to luminol‐based biosensors and their applications in clinical diagnosis, with emphasis on nanomaterials being employed as the improver.

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“…In comparison with the classical Ru(bpy) 3 2? , cyclometalated iridium(III) complexes generally possess much higher photoluminescence (PL) efficiency and easy tunability of the emission color. By modulating the ligand and changing the coordination mode, a series of cyclometalated iridium(III) complexes have been designed, synthesized, and thoroughly investigated through photophysics, electrochemistry, and ECL [23][24][25][26][27]. The development of new ECL emitters with different emission colors makes it possible to simultaneously determine multiplex analytes in clinical diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, miscellaneous nanomaterials, e.g., carbon nanodots, noble metal nanoclusters, and polymer dots, have emerged and aroused much attention as effective ECL emitters in recent years. The development of ECL emitters has been reviewed comprehensively previously [27,29], so it will not be covered here. In a more general sense, ECL has emerged as a very powerful analytical technique, and Ru(bpy) 3 2? , luminol and their derivatives have been successfully used in diverse fields, such as quantification of clinical biomarkers, food-borne pathogens, and biowarfare agents, as well as environmental monitoring.…”

Section: Introductionmentioning

confidence: 99%

Imaging Analysis Based on Electrogenerated Chemiluminescence

et al. 2017

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Electrochemiluminescence (ECL), also called electrogenerated chemiluminescence, is luminescence that is produced by chemical reactions triggered by electrochemical method. ECL imaging is a novel imaging technique, which can simultaneously provide two kinds of signals of both electrochemistry and optical image. Over the past two decades, ECL imaging has been not only a powerful tool for investigating the fundamental scientific questions such as ECL mechanisms and reaction kinetics, but also a versatile analytical technique for detection of a wide range of analytes including small molecules, DNA, proteins, and cells. In the first part of this review, we briefly describe the reaction mechanisms of ECL generation. Then the review focuses on the research progress on the ECL imaging approach. It is basically introduced from the following five aspects: (i) visualization of electroactive sites on surfaces, (ii) imaging analysis at the single-bead and single-cell levels, (iii) array bioanalysis, (iv) multi-color ECL imaging, and (v) paper chip based on ECL imaging. Finally, some perspectives and future directions in this active research area are presented.

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Recent Advances in Electrochemiluminescence Analysis

Cited by 475 publications

References 156 publications

Single Microbead‐Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens

Single Microbead‐Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens

Progress of the Electrochemiluminescence Biosensing Strategy for Clinical Diagnosis with Luminol as the Sensing Probe

Imaging Analysis Based on Electrogenerated Chemiluminescence

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